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ZP4 / ZBP

zona pellucida glycoprotein 4

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.

Gene Name: zona pellucida glycoprotein 4
Synonyms: ZP4, Zona pellucida B protein, Zona pellucida glycoprotein 4, Zp-4, Zona pellucida protein B, ZBP, ZPB
Target Sequences: NM_021186 NP_067009.1 Q12836

Publications (1)

1
Discovery of molecular markers to discriminate corneal endothelial cells in the human body. Masahito Yoshihara, Hiroko Ohmiya, Susumu Hara, Satoshi Kawasaki, FANTOM consortium; Yoshihide Hayashizaki, Masayoshi Itoh, Hideya Kawaji, Motokazu Tsujikawa, Kohji Nishida. PloS one. 2015 March;10:e0117581. [Full Text Article] [PubMed:25807145] [PMC:PMC4373821] Related Antibodies: LS-C160968.

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Antibodies (23)
Primary (23)
ZP4 / ZBP (23)
Human (23)
Mouse (4)
Rat (4)
Monkey (1)
IHC (8)
IHC-P (2)
WB (19)
ELISA (12)
IF (1)
Peptide-ELISA (3)
rabbit (23)
IgG (20)
polyclonal pc (23)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (12)
aa445-474 (7)
N-Terminus (4)
200-280 aa, Internal (1)
Internal (1)
aa218-267 (1)
aa231-280 (1)
aa277-322 (1)
No (22)
Yes (1)
ZP4 / ZBP Antibody - ZP4 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa445-474) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
ZP4 / ZBP Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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ZP4 / ZBP Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa445-474) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa445-474) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa445-474) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa445-474) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa445-474) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa445-474) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
ZP4 / ZBP Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human ovarian tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
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ZP4 / ZBP Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
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ZP4 / ZBP Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
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ZP4 / ZBP Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
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ZP4 / ZBP Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA
FITC Conjugated
50 µg/$294; 100 µg/$360
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ZP4 / ZBP Rabbit anti-Human Polyclonal (200-280 aa, Internal) Antibody
Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ZP4 / ZBP Antibody - Western blot analysis of extracts from Jurkat cells, using ZP4 antibody.
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ZP4 / ZBP Rabbit anti-Human Polyclonal Antibody
Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
ZP4 / ZBP Antibody - Western blot analysis of lysates from Jurkat cells, using ZP4 Antibody. The lane on the right is blocked with the synthesized peptide.
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa231-280) Antibody
Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
ZP4 / ZBP Antibody - Western blot analysis of the lysates from HepG2 cells using ZP4 antibody.
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa218-267) Antibody
Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
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ZP4 / ZBP Rabbit anti-Human Polyclonal (aa277-322) Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µl/$391
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ZP4 / ZBP Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
ZP4 / ZBP Antibody - Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ZP4 / ZBP Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
ZP4 / ZBP Antibody - Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ZP4 / ZBP Rabbit anti-Human Polyclonal (N-Terminus) (HRP) Antibody
Human
WB
HRP Conjugated
100 µl/$469
ZP4 / ZBP Antibody - Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ZP4 / ZBP Rabbit anti-Human Polyclonal (N-Terminus) (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$469
ZP4 / ZBP Antibody - Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ZP4 / ZBP Rabbit anti-Human Polyclonal (N-Terminus) (FITC) Antibody
Human
WB
FITC Conjugated
100 µl/$469
ZP4 / ZBP Antibody - Western blot analysis of ZP4 expression in HepG2 (A); HeLa (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
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ZP4 / ZBP Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human, Monkey
WB
Unconjugated
100 µl/$299
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).