ZAP70

zeta-chain (TCR) associated protein kinase 70kDa

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

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Products

Proteins (8)

Type

Over-Expression Lysate (3)

Recombinant (5)

Target

ZAP70 (8)

Publications

No (8)

Tag

6His, N-terminus (1)

GST (1)

GST, N-terminus (1)

His-T7 (2)

Myc-DDK (Flag) (3)

Species

Human (6)

Mouse (1)

Source

293T Cells (1)

E. coli (2)

HEK 293 Cells (3)

Wheat Germ Extract (2)

Purification

Purified (1)

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Human ZAP70 Protein (Recombinant GST) (Full Length) - LS-G30929

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

79.97 kDa

Price:

10 µg/$479; 25 µg/$670

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Human ZAP70 Protein (Recombinant GST, N-terminus) (aa397-493) - LS-G60541

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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ZAP70 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G120075

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

69.7 kDa

Price:

100 µg/$494

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Human ZAP70 Protein (Over-Expression Lysate 6His, N-terminus) (Pro2-Ala619) - LS-G26720

Source:

HEK 293 Cells

Tag:

6His, N-terminus

Molecular Weight:

71 kDa

Price:

100 µg/$298

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Human ZAP70 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G67246

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

69.7 kDa

Price:

20 µg/$1,107

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Human ZAP70 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G79411

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

69.7 kDa

Price:

20 µg/$150

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Mouse ZAP70 Protein (Recombinant His-T7) (aa270-560) - LS-G16303

Source:

E. coli

Tag:

His-T7

Molecular Weight:

37.0 kDa

Price:

10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309

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Human ZAP70 Protein (Recombinant His-T7) (aa337-600) - LS-G16307

Source:

E. coli

Tag:

His-T7

Molecular Weight:

34.2 kDa

Price:

10 µg/$292; 50 µg/$512; 100 µg/$817; 200 µg/$1,018; 1 mg/$2,168; 500 µg/$1,690; 5 mg/$3,571; 2 mg/$2,359

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