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YWHAE / 14-3-3 Epsilon

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon

YWHAE / 14-3-3 Epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Synonyms: YWHAE, 14-3-3E, 14-3-3 epsilon, 14-3-3 protein epsilon, MDCR, MDS, KCIP-1
Target Sequences: NM_006761 NP_006752.1 P62258

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Products
Proteins (11)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (2)
Recombinant (8)
YWHAE / 14-3-3 Epsilon (11)
No (11)
6His, N-terminus (2)
GST (2)
GST, N-terminus (2)
Myc-DDK (Flag) (3)
Human (9)
Mouse (1)
293T Cells (1)
E. coli (5)
HEK 293 Cells (4)
Wheat Germ Extract (1)
Purified (5)
Low endotoxin level (2)
YWHAE / 14-3-3 Epsilon Protein
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E. coli
None
100 µg/$411; 500 µg/$985; 20 µg/$276
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E. coli
GST, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
56kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,157
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E. coli
None
29.4 kDa
100 µg/$926
YWHAE / 14-3-3 Epsilon Protein - Purified recombinant protein YWHAE was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
29 kDa
20 µg/$1,107
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E. coli
GST, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
56.0 kDa
10 µg/$317; 50 µg/$524
YWHAE / 14-3-3 Epsilon Protein - 12.5% SDS-PAGE of human YWHAE stained with Coomassie Blue
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Wheat Germ Extract
GST
53.79 kDa
10 µg/$479; 25 µg/$670
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HEK 293 Cells
6His, N-terminus
40-50 kDa
200 µl/$283
YWHAE / 14-3-3 Epsilon Protein - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
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HEK 293 Cells
6His, N-terminus
28-30 kDa
50 µg/$360; 100 µg/$432; 250 µg/$726; 500 µg/$1,270
Select
293T Cells
Myc-DDK (Flag)
29 kDa
20 µg/$150
YWHAE / 14-3-3 Epsilon Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
29 kDa
100 µg/$494
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E. coli
GST
56.1 kDa
100 µg/$843
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).