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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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VRK2

vaccinia related kinase 2

Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.Isoform 2 phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.

Gene Name: vaccinia related kinase 2
Family/Subfamily: Protein Kinase , VRK
Synonyms: VRK2, Vaccinia related kinase 2, Vaccinia-related kinase 2
Target Sequences: NM_006296 NP_006287.2 Q86Y07

Filtered By: None
Target
VRK2 (17)
Reactivity
Human (9)
Mouse (10)
Application
IHC (1)
IHC-P (1)
WB (13)
ELISA (14)
IF (2)
IP (1)
Peptide-ELISA (1)
RNAi (1)
Host
rabbit (16)
mouse (1)
Isotype
IgG (15)
IgG1,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (16)
Clone
3B10 (1)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (8)
Epitope
aa354-381 (8)
140-220 aa, Internal (1)
aa171-220 (1)
Publications
No (17)
Showing 1-17 of 17 results

VRK2 Antibody (clone 3B10) LS-C198433
Mouse Monoclonal [clone 3B10] (IgG1,k) to Human VRK2
Human
ELISA IF RNAi WB
Unconjugated
100µg  $355.00
Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10 ug/ml]
VRK2 monoclonal antibody (M01), clone 3B10. Western Blot analysis of VRK2 expression in U-2 OS.
Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10 ug/ml]
VRK2 monoclonal antibody (M01), clone 3B10. Western Blot analysis of VRK2 expression in U-2 OS.

VRK2 Antibody LS-C681692
Rabbit Polyclonal (IgG) to Human VRK2
Human
ELISA IF IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

VRK2 Antibody (HRP) LS-C681696
Rabbit Polyclonal (IgG) to Human VRK2
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

VRK2 Antibody (Biotin) LS-C681694
Rabbit Polyclonal (IgG) to Human VRK2
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

VRK2 Antibody (FITC) LS-C681695
Rabbit Polyclonal (IgG) to Human VRK2
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00

VRK2 Antibody LS-C661580
Rabbit Polyclonal (IgG) to Human VRK2
Human
ELISA IP WB
Unconjugated
100µg  $435.00

VRK2 Antibody (aa354-381) LS-C326297
Rabbit Polyclonal (IgG) to Mouse VRK2
Mouse
ELISA WB
Unconjugated
200µl  $530.00

VRK2 Antibody (aa354-381, Biotin) LS-C273387
Rabbit Polyclonal (IgG) to Mouse VRK2
Mouse
ELISA WB
Biotin Conjugated
200µl  $680.00

VRK2 Antibody (aa354-381, FITC) LS-C273388
Rabbit Polyclonal (IgG) to Mouse VRK2
Mouse
ELISA WB
FITC Conjugated
200µl  $680.00

VRK2 Antibody (aa354-381, APC) LS-C273386
Rabbit Polyclonal (IgG) to Mouse VRK2
Mouse
ELISA WB
APC Conjugated
200µl  $680.00

VRK2 Antibody (aa354-381, PE) LS-C273390
Rabbit Polyclonal (IgG) to Mouse VRK2
Mouse
ELISA WB
PE Conjugated
200µl  $680.00

VRK2 Antibody (aa354-381, AP) LS-C273385
Rabbit Polyclonal (IgG) to Mouse VRK2
Mouse
ELISA WB
AP Conjugated
200µl  $680.00

VRK2 Antibody (aa354-381, Azide-free, HRP) LS-C273389
Rabbit Polyclonal (IgG) to Mouse VRK2
Mouse
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

VRK2 Antibody (140-220 aa, Internal) LS-C386652
Rabbit Polyclonal (IgG) to Human VRK2
Mouse, Human
ELISA WB
Unconjugated
100µl 1mg/ml  $265.00

VRK2 Antibody (aa171-220) LS-C120375
Rabbit Polyclonal (IgG) to Human VRK2
Mouse, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from Jurkat cells, using VRK2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using VRK2 Antibody. The lane on the right is blocked with the synthesized peptide.

VRK2 Antibody (aa354-381) LS-C158285
Rabbit Polyclonal to Mouse VRK2
Mouse
WB
Unconjugated
400µl  $305.00
Mouse Vrk2 Antibody western blot of mouse liver tissue lysates (35 ug/lane). The Vrk2 antibody detected the Vrk2 protein (arrow).
Mouse Vrk2 Antibody western blot of mouse liver tissue lysates (35 ug/lane). The Vrk2 antibody detected the Vrk2 protein (arrow).

VRK2 Antibody LS-C747397
Rabbit Polyclonal (IgG) to Human VRK2
Human
WB
Unconjugated
50µl  $235.00
Western blot analysis of extracts of LO2 cells, using VRK2 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Western blot analysis of extracts of LO2 cells, using VRK2 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Showing 1-17 of 17 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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