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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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VAV2

vav 2 guanine nucleotide exchange factor

Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.

Gene Name: vav 2 guanine nucleotide exchange factor
Synonyms: VAV2, Vav 2 oncogene, VAV-2
Target Sequences: NM_003371 NP_003362.2 P52735

Filtered By: None
Target
VAV2 (23)
Reactivity
Human (23)
Mouse (7)
Rat (2)
Bovine (1)
Dog (2)
Hamster (1)
Horse (1)
Monkey (1)
Pig (1)
Primate (1)
Application
IHC (9)
IHC-P (4)
WB (22)
Flo (2)
ELISA (12)
ICC (2)
IF (7)
IP (12)
Peptide-ELISA (3)
Host
rabbit (19)
mouse (1)
goat (1)
sheep (2)
Isotype
Ig (1)
IgG (13)
IgG1 (1)
Clonality
monoclonal mc (1)
polyclonal pc (22)
Clone
3F5D3 (1)
Format
AP Conjugated (1)
APC Conjugated (1)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
PE Conjugated (1)
Unconjugated (17)
Epitope
N-Terminus (3)
80-160 aa (2)
Asp138 (1)
C-Terminus (1)
aa108-157 (1)
aa208-221 (1)
aa760-810 (1)
pTyr142 (1)
Publications
No (23)
Showing 1-23 of 23 results

VAV2 Antibody (aa208-221) IHC-plus™ LS-B6444
Goat Polyclonal to Human VAV2
Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-VAV2 antibody IHC of human colon, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Staining (1.5 ug/ml) of 293 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Anti-VAV2 antibody IHC of human colon, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Staining (1.5 ug/ml) of 293 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

VAV2 Antibody (N-Terminus) LS-C368550
Rabbit Polyclonal to Human VAV2
Human
ICC IF IHC IHC-P WB
Unconjugated
30µl 1mg/ml  $215.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $325.00
Immunohistochemical analysis of VAV2 (pY142) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of VAV2 (pY142) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of VAV2 (pY142) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of VAV2 (pY142) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

VAV2 Antibody (phospho-Tyr142) LS-C199715
Rabbit Polyclonal (IgG) to Human VAV2
Mouse, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of NIH/3T3 cells, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of NIH/3T3 cells, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide.

VAV2 Antibody (80-160 aa) LS-C381536
Rabbit Polyclonal (IgG) to Human VAV2
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

VAV2 Antibody (aa108-157) LS-C118034
Rabbit Polyclonal (IgG) to Human VAV2
Mouse, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using VAV2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of COS7 cells, using VAV2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using VAV2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of COS7 cells, using VAV2 Antibody. The picture on the right is blocked with the synthesized peptide.

VAV2 Antibody (80-160 aa) LS-C386626
Rabbit Polyclonal (IgG) to Human VAV2
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Vav2 antibody
Western blot of Vav2 antibody

VAV2 Antibody (N-Terminus) LS-C352980
Rabbit Polyclonal to Human VAV2
Human
ICC IF IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunofluorescent analysis of VAV2 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of VAV2 expression in A431 (A); THP1 (B); NIH3T3 (C) whole cell lysates.
Immunofluorescent analysis of VAV2 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of VAV2 expression in A431 (A); THP1 (B); NIH3T3 (C) whole cell lysates.

VAV2 Antibody LS-C661533
Rabbit Polyclonal (IgG) to Human VAV2
Human
ELISA IHC IP WB
Unconjugated
100µg  $435.00

VAV2 Antibody (Asp138) LS-C176997
Rabbit Polyclonal to Human VAV2
Mouse, Human
IF IHC WB
Unconjugated
100µl  $345.00
Western blot of VAV2 (D138) pAb in extracts from THP1 cells.
Western blot of VAV2 (D138) pAb in extracts from THP1 cells.

VAV2 Antibody (N-Terminus) LS-C757628
Rabbit Polyclonal (Ig) to Human VAV2
Human
ELISA Flo WB
Unconjugated
50µl  $215.00; 200µl  $325.00
All lanes: Anti-VAV2 Antibody (N-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained with VAV2 Antibody (N-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (VAV2 Antibody (N-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes: Anti-VAV2 Antibody (N-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained with VAV2 Antibody (N-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (VAV2 Antibody (N-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

VAV2 Antibody LS-C411017
Rabbit Polyclonal (IgG) to Human VAV2
Mouse, Rat, Human
IHC IP WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00

VAV2 Antibody (clone 3F5D3) LS-C669237
Mouse Monoclonal [clone 3F5D3] (IgG1) to Human VAV2
Human
ELISA Flo
Unconjugated
0.1mg  $365.00

VAV2 Antibody (C-Terminus) LS-C11015
Rabbit Polyclonal (IgG) to Human VAV2
Human
ELISA IP WB
Unconjugated
100µg  $605.00

VAV2 Antibody (HRP) LS-C550257
Rabbit Polyclonal (IgG) to Human VAV2
Human
ELISA IP WB
HRP Conjugated
100µl  $740.00

VAV2 Antibody (Biotin) LS-C530170
Rabbit Polyclonal (IgG) to Human VAV2
Human
ELISA IP WB
Biotin Conjugated
100µl  $740.00

VAV2 Antibody (FITC) LS-C540086
Rabbit Polyclonal (IgG) to Human VAV2
Human
ELISA IP WB
FITC Conjugated
100µl  $740.00

VAV2 Antibody (AP) LS-C512066
Rabbit Polyclonal (IgG) to Human VAV2
Human
ELISA IP WB
AP Conjugated
100µl  $740.00

VAV2 Antibody (APC) LS-C519848
Rabbit Polyclonal (IgG) to Human VAV2
Human
ELISA IP WB
APC Conjugated
100µl  $740.00

VAV2 Antibody (PE) LS-C658223
Rabbit Polyclonal (IgG) to Human VAV2
Human
ELISA IP WB
PE Conjugated
100µl  $740.00

VAV2 Antibody LS-C26557
Sheep Polyclonal to Human VAV2
Human
IP WB
Unconjugated
250µg  $355.00
Legend: HEK293 cells were transfected with either empty expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300 ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300 ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300 ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300 ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300 ug lysate). Western blot Ab - SHD anti Vav2 antibody 1:10000.
Legend: HEK293 cells were transfected with either empty expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300 ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300 ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300 ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300 ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300 ug lysate). Western blot Ab - SHD anti Vav2 antibody 1:10000.

VAV2 Antibody LS-C26556
Sheep Polyclonal to Human VAV2
Human
IP WB
Unconjugated
250µg  $355.00
Legend: HEK293 cells were transfected with either empty expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300 ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 u g lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 l/300 g lysate). DPH or SHD alpha Vav2 antiserum (2.5 l/300 g lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 l/300 g lysate). DPH or SHD anti Vav2 antisera (2.5 l/300 g lysate). Western blot Ab - SHD anti Vav2 antibody 1:10000.
Legend: HEK293 cells were transfected with either empty expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300 ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 u g lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 l/300 g lysate). DPH or SHD alpha Vav2 antiserum (2.5 l/300 g lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 l/300 g lysate). DPH or SHD anti Vav2 antisera (2.5 l/300 g lysate). Western blot Ab - SHD anti Vav2 antibody 1:10000.

VAV2 Antibody LS-C357024
Rabbit Polyclonal to Human VAV2
Human
WB
Unconjugated
100µl  $305.00
Anti-VAV2 Antibody at 1:1000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-VAV2 Antibody at 1:1000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

VAV2 Antibody (aa760-810) LS-C359394
Rabbit Polyclonal to Human VAV2
Dog, Human, Primate
WB
Unconjugated
100µg 0.5mg/ml  $335.00
Western Blot: VAV2 Antibody - analysis of VAV2. Jurkat lysate in the 1) absence and 2) presence of immunizing peptide, and 3) K562 lysate probed with 3 ug/ml of VAV2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: VAV2 Antibody - analysis of VAV2. Jurkat lysate in the 1) absence and 2) presence of immunizing peptide, and 3) K562 lysate probed with 3 ug/ml of VAV2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Showing 1-23 of 23 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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