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URM1

ubiquitin related modifier 1

Acts as a sulfur carrier required for 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm5S2U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.

Gene Name: ubiquitin related modifier 1
Synonyms: URM1, C9orf74, RP11-339B21.4, Ubiquitin related modifier 1
Target Sequences: NM_030914 NP_112176.1 Q9BTM9

☰ Filters
Products
Antibodies (13)
Primary (13)
URM1 (13)
Human (11)
Mouse (1)
Rat (3)
Yeast (1)
IHC (8)
IHC-P (1)
WB (13)
ELISA (8)
Peptide-ELISA (1)
rabbit (12)
mouse (1)
IgG (11)
IgM,k (1)
monoclonal mc (1)
polyclonal pc (12)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
PE Conjugated (1)
Unconjugated (8)
aa8-40 (7)
No (13)
URM1 Antibody - Western blot of Urm1 fusion protein. Anti- Urm1 antibody generated by immunization with recombinant yeast Urm1 was tested by western blot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX  Panel C shows specific reaction with Urm1 using a 1:1,000 dilution of IgG fraction of Rabbit-anti- Urm1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX  All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Urm1 is highly specific and does not cross react with other UBLs. Bands present in Panel C indicate that Urm1 and conjugated Urm1 is present in most yeast cell lysates albeit at significantly reduced levels relative to the Urm1-GFP transfected lysate. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov,
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URM1 Rabbit anti-Yeast Polyclonal Antibody
Yeast
ELISA, WB
Unconjugated
500 µg/$606
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URM1 Rabbit anti-Human Polyclonal (aa8-40) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
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URM1 Rabbit anti-Human Polyclonal (aa8-40) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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URM1 Rabbit anti-Human Polyclonal (aa8-40) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
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URM1 Rabbit anti-Human Polyclonal (aa8-40) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
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URM1 Rabbit anti-Human Polyclonal (aa8-40) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
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URM1 Rabbit anti-Human Polyclonal (aa8-40) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
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URM1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$341
URM1 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with URM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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URM1 Rabbit anti-Human Polyclonal (aa8-40) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
URM1 Antibody - Western blot analysis URM1 using LOVO cells whole cells lysates
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URM1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
URM1 Antibody - URM1 antibody western blot of HepG2 cell line lysates (35 ug/lane). The URM1 antibody detected the URM1 protein (arrow).
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URM1 Mouse anti-Human Monoclonal Antibody
Human
WB
Unconjugated
400 µl/$393
URM1 Antibody - Western blot analysis of extracts of rat eye, using URM1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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URM1 Rabbit anti-Human Polyclonal Antibody
Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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URM1 Rabbit anti-Rat Polyclonal Antibody
Rat
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).