Ubiquitin

Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It was discovered in 1975 by Goldstein and further characterized throughout the 1970s and 1980s. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52 and RPS27A. Ubiquitination is a post-translational modification (an addition to a protein after it has been made) where ubiquitin is attached to a substrate protein. The addition of ubiquitin can affect proteins in many ways: It can signal for their degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The result of this sequential cascade binds ubiquitin to lysine residues on the protein substrate via an isopeptide bond or to the amino group of the protein's N-terminus via a peptide bond. The protein modifications can be either a single ubiquitin protein (monoubiquitination) or a chain of ubiquitin (polyubiquitination). The ubiquitination bonds are always formed with one of the seven lysine residues from the ubiquitin molecule. These 'linking' lysines are represented by a "K" (which is the one-letter amino acid notation of lysine) and a number, referring to its position in the ubiquitin molecule. First, a ubiquitin molecule is bonded by its C-terminus to a specific lysine residue (e.g. K48, K29, K63,…) on the target protein. Poly-ubiquitination occurs when the C-terminus of another ubiquitin, will be linked again to a lysine residue (for example again K48 or K29) on the previously added ubiquitin molecule, forming a chain. This process repeats several times, leading to the addition of several ubiquitins. Only poly-ubiquitination on defined lysines, mostly on K48 and K29, is related to degradation with the proteasome (referred to as the "molecular kiss of death"), while other polyubiquitinations (e.g. on K63, K11, K6) and monoubiquitinations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair.

Ubiquitin Target Details

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Products

ELISA Kits (14)

Type

Competitive EIA (3)

Development Kit (3)

Sandwich (6)

Sandwich CLIA (2)

Target

Ubiquitin (14)

Reactivity

Human (4)

Mouse (4)

Rat (2)

Chicken (1)

Monkey (1)

Porcine (1)

Rabbit (1)

Product Group

DevKit DuoPlus (3)

Format

12 x 8-Well Microstrips (2)

96-Well Microplate (2)

96-Well Strip Plate (7)

Antibody Pair and Standard (3)

Publications

No (14)

Sample Type

Plasma (11)

Serum (11)

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Mouse Ubiquitin (Sandwich ELISA) ELISA Kit - LS-F23540

Type:

Sandwich

Format:

96-Well Microplate

Reactivity:

Mouse

Range:

31.25 - 2000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$651

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Human Ubiquitin (Sandwich ELISA) ELISA Kit - LS-F23795

Type:

Sandwich

Format:

96-Well Microplate

Reactivity:

Human

Range:

62.5 - 4000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$651

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Human Ubiquitin (Competitive EIA) ELISA Kit - LS-F27303

Type:

Competitive EIA

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

25 - 500 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$913

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Monkey Ubiquitin (Sandwich ELISA) ELISA Kit - LS-F48911

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Monkey

Range:

62.5 - 4000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$728

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Chicken Ubiquitin (Sandwich ELISA) ELISA Kit - LS-F48910

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Chicken

Range:

3.125 - 200 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Rabbit Ubiquitin (Sandwich ELISA) ELISA Kit - LS-F48913

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rabbit

Range:

2.5 - 160 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Porcine Ubiquitin (Sandwich ELISA) ELISA Kit - LS-F48914

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Porcine

Range:

62.5 - 4000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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DevKit DuoPlus Mouse Ubiquitin ELISA Development Kit - LS-F71551

Type:

Development Kit

Format:

Antibody Pair and Standard

Reactivity:

Mouse

Range:

12.5 - 800 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

500 Wells/$1,178; 1000 Wells/$1,739

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DevKit DuoPlus Human Ubiquitin ELISA Development Kit - LS-F71554

Type:

Development Kit

Format:

Antibody Pair and Standard

Reactivity:

Human

Range:

12.5 - 800 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

500 Wells/$1,178; 1000 Wells/$1,739

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DevKit DuoPlus Rat Ubiquitin ELISA Development Kit - LS-F71550

Type:

Development Kit

Format:

Antibody Pair and Standard

Reactivity:

Rat

Range:

12.5 - 800 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

500 Wells/$1,178; 1000 Wells/$1,739

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Mouse Ubiquitin (CLIA) ELISA Kit - LS-F31013

Type:

Sandwich CLIA

Format:

12 x 8-Well Microstrips

Reactivity:

Mouse

Range:

12.5 - 800 pg/ml

Detection:

Chemiluminescent

Sample Types:

Plasma, Serum

Price:

1 Plate/$728

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Human Ubiquitin (CLIA) ELISA Kit - LS-F29806

Type:

Sandwich CLIA

Format:

12 x 8-Well Microstrips

Reactivity:

Human

Range:

15.625 - 1000 pg/ml

Detection:

Chemiluminescent

Sample Types:

Plasma, Serum

Price:

1 Plate/$728

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Mouse Ubiquitin (Competitive EIA) ELISA Kit - LS-F28648

Type:

Competitive EIA

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

1 - 25 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$913

Select

Rat Ubiquitin (Competitive EIA) ELISA Kit - LS-F28102

Type:

Competitive EIA

Format:

96-Well Strip Plate

Reactivity:

Rat

Range:

250 - 5000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$913

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