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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UBE2S / E2 EPF

ubiquitin-conjugating enzyme E2S

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

Gene Name: ubiquitin-conjugating enzyme E2S
Synonyms: UBE2S, E2 EPF, E2-EPF, E2EPF, EPF5, E2-EPF5, Ubiquitin-protein ligase S, Ubiquitin carrier protein S
Target Sequences: NM_014501 NP_055316.2 Q16763

Filtered By: None
Target
UBE2S / E2 EPF (53)
Reactivity
Human (53)
Mouse (10)
Rat (8)
Bovine (7)
Chimpanzee (3)
Dog (3)
Gibbon (3)
Guinea pig (4)
Horse (6)
Monkey (6)
Pig (5)
Rabbit (5)
Xenopus (7)
Zebrafish (1)
Application
IHC (30)
IHC-P (4)
WB (49)
Flo (6)
ELISA (29)
ICC (10)
IF (6)
IP (10)
Peptide-ELISA (1)
Host
rabbit (44)
mouse (8)
goat (1)
Isotype
IgG (30)
IgG1 (5)
IgG1,k (1)
IgG2a,k (1)
Clonality
monoclonal mc (8)
polyclonal pc (45)
Clone
1B5 (1)
2G7 (1)
4B8 (1)
4E4 (1)
4G2 (1)
4H3 (1)
Format
AP Conjugated (3)
APC (1)
APC Conjugated (4)
Azide-free (3)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (4)
Unconjugated (25)
Epitope
aa10-40 (8)
aa183-210 (8)
aa192-222 (8)
N-Terminus (4)
aa1-222 (1)
aa1-50 (1)
aa172-222 (1)
aa2-14 (1)
Publications
No (53)
Showing 1-25 of 53 results

UBE2S / E2 EPF Antibody (aa183-210) IHC-plus™ LS-B13775
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
IHC IHC-P WB
Unconjugated
400µl  $485.00
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
UBE2S Antibody western blot of HL-60 cell line lysates (35 ug/lane). The UBE2S antibody detected the UBE2S protein (arrow).
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
UBE2S Antibody western blot of HL-60 cell line lysates (35 ug/lane). The UBE2S antibody detected the UBE2S protein (arrow).

UBE2S / E2 EPF Antibody (aa10-40) LS-C99505
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of E2EPF (arrow) using E2EPF Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of E2EPF (arrow) using E2EPF Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).

UBE2S / E2 EPF Antibody LS-C113040
Mouse Monoclonal to Human UBE2S / E2 EPF
Human
ELISA Flo ICC IF WB
Unconjugated
50µl 1mg/ml  $315.00

UBE2S / E2 EPF Antibody (aa1-222) LS-C132795
Mouse Monoclonal (IgG2a,k) to Human UBE2S / E2 EPF
Human
ELISA ICC IF WB
Unconjugated
50µl  $425.00

UBE2S / E2 EPF Antibody (clone 4G2) LS-C174207
Mouse Monoclonal [clone 4G2] (IgG1) to Human UBE2S / E2 EPF
Human
Flo IF WB
Unconjugated
100µl 1mg/ml  $335.00
Anti-UBE2S mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
Anti-UBE2S mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.

UBE2S / E2 EPF Antibody (clone 4H3) LS-C337835
Mouse Monoclonal [clone 4H3] (IgG1) to Human UBE2S / E2 EPF
Mouse, Human, Monkey
Flo IF WB
Unconjugated
100µl 1mg/ml  $345.00
Anti-UBE2S mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S.
Western blot of extracts (35ug) from 9 different cell lines by using anti-UBE2S monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-UBE2S mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S.
Western blot of extracts (35ug) from 9 different cell lines by using anti-UBE2S monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

UBE2S / E2 EPF Antibody LS-C663482
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
ICC IHC IP WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00; 10ml 0.5mg/ml  $2,100.00
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.

UBE2S / E2 EPF Antibody (HRP) LS-C713094
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
ICC IHC IP WB
HRP Conjugated
20µl  $215.00; 100µl  $285.00; 200µl  $345.00; 1ml  $680.00; 10ml  $2,730.00
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.

UBE2S / E2 EPF Antibody (Biotin) LS-C688358
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
ICC IHC IP WB
Biotin Conjugated
20µl  $205.00; 100µl  $265.00; 200µl  $315.00; 1ml  $575.00; 10ml  $2,310.00
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.

UBE2S / E2 EPF Antibody (FITC) LS-C695305
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
ICC IHC IP WB
FITC Conjugated
20µl  $215.00; 100µl  $275.00; 200µl  $325.00; 1ml  $635.00; 10ml  $2,520.00
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.

UBE2S / E2 EPF Antibody (Cy3) LS-C704068
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
ICC IHC IP WB
Cy3 Conjugated
20µl  $245.00; 100µl  $355.00; 200µl  $445.00; 1ml  $1,050.00; 10ml  $4,200.00
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.

UBE2S / E2 EPF Antibody (PE) LS-C727157
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
ICC IHC IP WB
PE Conjugated
20µl  $215.00; 100µl  $275.00; 200µl  $325.00; 1ml  $635.00; 10ml  $2,520.00
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.

UBE2S / E2 EPF Antibody (APC) LS-C741365
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
ICC IHC IP WB
APC Conjugated
20µl  $225.00; 100µl  $305.00; 200µl  $375.00; 1ml  $785.00
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.

UBE2S / E2 EPF Antibody (APC, Cy7) LS-C736076
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
ICC IHC IP WB
APC, Cy7 Conjugated
20µl  $285.00; 100µl  $455.00; 200µl  $635.00; 1ml  $1,575.00; 10ml  $6,300.00
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.
DAB staining on fromalin fixed paraffin- embedded breast cancer tissue
Western Blot; Sample: Recombinant protein.

UBE2S / E2 EPF Antibody LS-C401617
Rabbit Polyclonal (IgG) to Human UBE2S / E2 EPF
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human thyroid cancer using UBE2S Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human brain using UBE2S Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human thyroid cancer using UBE2S Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human brain using UBE2S Polyclonal Antibody at dilution of 1:40.

UBE2S / E2 EPF Antibody (clone 1B5) LS-C174195
Mouse Monoclonal [clone 1B5] (IgG1) to Human UBE2S / E2 EPF
Human
Flo WB
Unconjugated
100µl 1mg/ml  $335.00
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2S antibody, and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2S antibody, and then analyzed by flow cytometry.

UBE2S / E2 EPF Antibody (clone 4B8) LS-C174196
Mouse Monoclonal [clone 4B8] (IgG1) to Human UBE2S / E2 EPF
Human
Flo WB
Unconjugated
100µl 1mg/ml  $335.00
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2S antibody, and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2S antibody, and then analyzed by flow cytometry.

UBE2S / E2 EPF Antibody (aa192-222) LS-C99506
Rabbit Polyclonal to Human UBE2S / E2 EPF
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
E2EPF Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of E2EPF (arrow) using E2EPF Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the E2EPF gene (Lane 2) (Origene Technologies).
E2EPF Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of E2EPF (arrow) using E2EPF Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the E2EPF gene (Lane 2) (Origene Technologies).

UBE2S / E2 EPF Antibody (aa10-40) LS-C329010
Rabbit Polyclonal (IgG) to Human UBE2S / E2 EPF
Human
ELISA IHC WB
Unconjugated
200µl  $530.00

UBE2S / E2 EPF Antibody (aa192-222) LS-C329011
Rabbit Polyclonal (IgG) to Human UBE2S / E2 EPF
Human
ELISA IHC WB
Unconjugated
200µl  $530.00

UBE2S / E2 EPF Antibody LS-C661386
Rabbit Polyclonal (IgG) to Human UBE2S / E2 EPF
Human
ELISA IHC WB
Unconjugated
100µg  $435.00

UBE2S / E2 EPF Antibody LS-C661389
Rabbit Polyclonal (IgG) to Human UBE2S / E2 EPF
Human
ELISA IHC WB
Unconjugated
100µg  $435.00

UBE2S / E2 EPF Antibody (aa2-14) LS-C54752
Goat Polyclonal to Human UBE2S / E2 EPF
Xenopus, Rat, Pig, Mouse, Dog, Bovine, Human, Monkey
Flo IF Peptide-ELISA
Unconjugated
100µg 0.5mg/ml  $335.00

UBE2S / E2 EPF Antibody LS-C676674
Rabbit Polyclonal (IgG) to Human UBE2S / E2 EPF
Human
ELISA IHC IHC-P
Unconjugated
50µl  $235.00; 100µl  $285.00
Immunohistochemistry of paraffin-embedded human melanoma at dilution 1:100
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human melanoma at dilution 1:100
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100

UBE2S / E2 EPF Antibody (clone 4E4) LS-C174206
Mouse Monoclonal [clone 4E4] (IgG1) to Human UBE2S / E2 EPF
Human
IF WB
Unconjugated
100µl 1mg/ml  $335.00
Anti-UBE2S mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
Anti-UBE2S mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
Showing 1-25 of 53 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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