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UBE2M / UBC12

ubiquitin-conjugating enzyme E2M

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Gene Name: ubiquitin-conjugating enzyme E2M
Synonyms: UBE2M, NEDD8 protein ligase, NEDD8 carrier protein, NEDD8-conjugating enzyme Ubc12, HUbc12, UBC-RS2, UBC12, Ubiquitin-protein ligase M, Yeast UBC12 homolog, Ubiquitin carrier protein M
Target Sequences: NM_003969 NP_003960.1 P61081

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Products
Proteins (7)
Over-Expression Lysate (2)
Recombinant (5)
UBE2M / UBC12 (7)
No (7)
GST (1)
His (1)
Myc-DDK (Flag) (2)
Human (6)
293T Cells (1)
E. coli (4)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (2)
Low endotoxin level (2)
UBE2M / UBC12 Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
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E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
20.9kD
10 µg/$233; 50 µg/$277; 500 µg/$725; 1 mg/$1,113
UBE2M / UBC12 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
20.7 kDa
20 µg/$150
UBE2M / UBC12 Protein - 12.5% SDS-PAGE of human UBE2M stained with Coomassie Blue
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Wheat Germ Extract
GST
45.87 kDa
10 µg/$479; 25 µg/$670
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E. coli
None
21 kDa
100 µg/$661
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
20.9 kDa
10 µg/$322; 50 µg/$365
UBE2M / UBC12 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
20.7 kDa
100 µg/$494
Viewing 1-7 of 7 product results


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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).