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UBE1DC1 / UBA5

ubiquitin-like modifier activating enzyme 5

UBE1DC1 / UBA5 is a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified.

Gene Name: ubiquitin-like modifier activating enzyme 5
Synonyms: UBA5, THIFP1, Ubiquitin-activating enzyme 5, UBE1DC1, UFM1-activating enzyme
Target Sequences: NM_024818 NP_079094.1 Q9GZZ9

☰ Filters
Antibodies (21)
Proteins (9)
Over-Expression Lysate (4)
Primary (21)
Recombinant (5)
UBE1DC1 / UBA5 (30)
Human (20)
Mouse (12)
Rat (7)
Bat (6)
Bovine (6)
Chicken (3)
Chimpanzee (6)
Dog (3)
Drosophila (3)
Gibbon (6)
Guinea pig (6)
Horse (6)
Insect (3)
Monkey (7)
Orangutan (6)
Pig (6)
Rabbit (6)
Xenopus (3)
Zebrafish (6)
IHC (4)
IHC-P (2)
WB (20)
Flo (1)
ELISA (7)
IF (2)
IP (3)
Peptide-ELISA (2)
rabbit (21)
Product Group
IHCPlus (1)
IgG (16)
polyclonal pc (21)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
Unconjugated (12)
aa122-171 (4)
aa99-148 (4)
250-330 aa, C-terminal (1)
C-Terminus (1)
aa125-175 (1)
aa281-330 (1)
aa325-375 (1)
No (30)
6His, N-terminus (2)
GST (1)
His (1)
Myc-DDK (Flag) (5)
Human (7)
293T Cells (2)
E. coli (3)
HEK 293 Cells (3)
Wheat Germ Extract (1)
Purified (3)
Low endotoxin level (2)
UBE1DC1 / UBA5 Antibody - Anti-UBA5 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (aa281-330) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$440
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100 µg/$351
UBE1DC1 / UBA5 Antibody - Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (aa122-171) Antibody
Human
WB
Unconjugated
100 µl/$424
UBE1DC1 / UBA5 Antibody - Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (aa122-171) (HRP) Antibody
Xenopus, Chicken, Zebrafish, Insect, Drosophila, Pig, Horse, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
WB
HRP Conjugated
100 µl/$469
UBE1DC1 / UBA5 Antibody - Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (aa122-171) (Biotin) Antibody
Xenopus, Chicken, Zebrafish, Insect, Drosophila, Pig, Horse, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
UBE1DC1 / UBA5 Antibody - Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (aa122-171) (FITC) Antibody
Xenopus, Chicken, Zebrafish, Insect, Drosophila, Pig, Horse, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
WB
FITC Conjugated
100 µl/$469
UBE1DC1 / UBA5 Antibody - Western blot analysis of extracts of various cell lines, using UBA5 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 15s.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
UBE1DC1 / UBA5 Protein - 12.5% SDS-PAGE of human UBE1DC1 stained with Coomassie Blue
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Wheat Germ Extract
GST
71.3 kDa
10 µg/$479; 25 µg/$670
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E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
47.0 kDa
10 µg/$376; 50 µg/$526
UBE1DC1 / UBA5 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
UBE1DC1 / UBA5 Antibody - Western blot of Uba5 antibody
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (250-330 aa, C-terminal) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
UBE1DC1 / UBA5 Antibody - Immunohistochemical analysis of UBA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human, Monkey
IHC, IHC-P, WB
Unconjugated
100 µl/$299
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
UBE1DC1 / UBA5 Antibody - Western blot. All lanes: UBA5 antibody at 6 ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: HepG-2 whole cell lysate. Lane 4: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
UBE1DC1 / UBA5 Antibody - Detection of Human UBA5 by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat, 293T, and HeLa cells. Antibodies: Affinity purified rabbit anti-UBA5 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (aa325-375) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
UBE1DC1 / UBA5 Antibody - UBE1DC1 / UBA5 antibody Western Blot of Mouse Thymus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE1DC1 / UBA5 Rabbit anti-Mouse Polyclonal (aa99-148) Antibody
Mouse
WB
Unconjugated
100 µl/$424
UBE1DC1 / UBA5 Antibody - UBE1DC1 / UBA5 antibody Western Blot of Mouse Thymus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE1DC1 / UBA5 Rabbit anti-Mouse Polyclonal (aa99-148) (HRP) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Guinea pig, Bovine, Bat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
WB
HRP Conjugated
100 µl/$469
UBE1DC1 / UBA5 Antibody - UBE1DC1 / UBA5 antibody Western Blot of Mouse Thymus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE1DC1 / UBA5 Rabbit anti-Mouse Polyclonal (aa99-148) (Biotin) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Guinea pig, Bovine, Bat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
UBE1DC1 / UBA5 Antibody - UBE1DC1 / UBA5 antibody Western Blot of Mouse Thymus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE1DC1 / UBA5 Rabbit anti-Mouse Polyclonal (aa99-148) (FITC) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Guinea pig, Bovine, Bat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
WB
FITC Conjugated
100 µl/$469
Select
UBE1DC1 / UBA5 Rabbit anti-Human Polyclonal (aa125-175) Antibody
Human
IP
Unconjugated
10 µg/$295; 100 µl/$514
UBE1DC1 / UBA5 Protein - Purified recombinant protein UBA5 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
44.7 kDa
20 µg/$1,107
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293T Cells
Myc-DDK (Flag)
44.7 kDa
20 µg/$150
Viewing 1-25 of 30 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).