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UBA1 / UBE1

ubiquitin-like modifier activating enzyme 1

Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the Cterminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.

Gene Name: ubiquitin-like modifier activating enzyme 1
Synonyms: UBA1, A1S9, A1ST, A1S9T, AMCX1, GXP1, Protein A1S9, SMAX2, Ubiquitin-activating enzyme E1, UBE1X, POC20, UBE1, UBA1A
Target Sequences: NM_003334 NP_003325.2 P22314

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Products
Proteins (8)
Over-Expression Lysate (4)
Recombinant (4)
UBA1 / UBE1 (8)
No (8)
GST (1)
GST, N-terminus (1)
His-GST (1)
Myc-DDK (Flag) (5)
Human (6)
293T Cells (2)
Baculovirus (1)
HEK 293 Cells (3)
Wheat Germ Extract (2)
Purified (2)
Low endotoxin level (1)
UBA1 / UBE1 Protein - Purified recombinant protein UBA1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
117.7 kDa
20 µg/$1,107
UBA1 / UBE1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
UBA1 / UBE1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
117.7 kDa
20 µg/$150
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293T Cells
Myc-DDK (Flag)
117.7 kDa
20 µg/$150
UBA1 / UBE1 Protein - 12.5% SDS-PAGE of human UBA1 stained with Coomassie Blue
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Wheat Germ Extract
GST
144.2 kDa
10 µg/$479; 25 µg/$670
UBA1 / UBE1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
117.7 kDa
100 µg/$494
Select
Baculovirus
His-GST
Less than 1.0 EU/µg protein (determined by LAL method).
146 kDa
50 µg/$502
UBA1 / UBE1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
117.7 kDa
100 µg/$494
Viewing 1-8 of 8 product results


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If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).