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TRIM25

tripartite motif containing 25

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Gene Name: tripartite motif containing 25
Family/Subfamily: Tripartite Motif
Synonyms: TRIM25, EFP, Zinc finger protein 147, Z147, Zinc finger protein-147, ZNF147, Tripartite motif containing 25, RING finger protein 147, RNF147, Tripartite motif-containing 25
Target Sequences: NM_005082 NP_005073.2 Q14258

☰ Filters
Products
Antibodies (41)
Primary (41)
TRIM25 (41)
Human (41)
Mouse (8)
Rat (6)
Bovine (6)
Chimpanzee (6)
Dog (3)
Gibbon (9)
Guinea pig (3)
Hamster (3)
Monkey (7)
Orangutan (3)
Rabbit (3)
IHC (10)
IHC-P (4)
WB (37)
Flo (2)
ELISA (19)
ICC (2)
IF (8)
IP (5)
Peptide-ELISA (2)
rabbit (38)
mouse (3)
Product Group
IHCPlus (2)
IgG (23)
IgG1,k (1)
IgG2b (2)
monoclonal mc (3)
polyclonal pc (38)
5B5B10 (1)
5B5B12 (1)
5C3 (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (5)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (1)
Unconjugated (24)
aa277-306 (7)
aa251-300 (4)
aa337-386 (4)
aa359-408 (4)
180-260 aa, Internal (1)
Asp222 (1)
Internal (1)
aa201-250 (1)
aa325-375 (1)
aa375-425 (1)
aa75-125 (1)
No (41)
TRIM25 Antibody - Anti-TRIM25 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
TRIM25 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
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TRIM25 Mouse anti-Human Monoclonal (5C3) Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µg/$460
TRIM25 Antibody - Immunofluorescence analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
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TRIM25 Mouse anti-Human Monoclonal (5B5B12) Antibody
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
100 µl/$459
TRIM25 Antibody - Immunofluorescence analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
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TRIM25 Mouse anti-Human Monoclonal (5B5B10) Antibody
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
100 µl/$459
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TRIM25 Rabbit anti-Human Polyclonal (aa75-125) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
TRIM25 Antibody - Immunohistochemistry of paraffin-embedded human colon cancer using TRIM25 Antibody at dilution of 1:100
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$294; 100 µg/$360
TRIM25 Antibody - Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 5 µg/ml.
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, ICC, IF, WB
Unconjugated
0.1 mg/$460
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IP, WB
Unconjugated
100 µg/$351
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TRIM25 Rabbit anti-Human Polyclonal (180-260 aa, Internal) Antibody
Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
TRIM25 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Human, Monkey
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
TRIM25 Antibody - IHC of paraffin-embedded Colon ca, using TRIM25 antibody at 1:500 dilution.
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$450
TRIM25 Antibody - Immunohistochemical analysis of TRIM25 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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TRIM25 Rabbit anti-Human Polyclonal (Internal) Antibody
Human
IHC, IHC-P, WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
TRIM25 Antibody - Immunohistochemistry of paraffin-embedded Human breast cancer using TRIM25 Polyclonal Antibody at dilution of 1:40.
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
TRIM25 Antibody - Western blot of EFP (D222) pAb in extracts from K562 cells.
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TRIM25 Rabbit anti-Human Polyclonal (Asp222) Antibody
Human
IHC, WB
Unconjugated
50 µl/$321; 100 µl/$403
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TRIM25 Rabbit anti-Human Polyclonal (aa277-306) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
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TRIM25 Rabbit anti-Human Polyclonal (aa277-306) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
TRIM25 Rabbit anti-Human Polyclonal (aa277-306) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
TRIM25 Rabbit anti-Human Polyclonal (aa277-306) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
TRIM25 Rabbit anti-Human Polyclonal (aa277-306) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
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TRIM25 Rabbit anti-Human Polyclonal (aa277-306) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
TRIM25 Antibody - Western blot analysis of extracts from K562 cells, using ZNF147 antibody.
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$357
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TRIM25 Rabbit anti-Human Polyclonal (aa375-425) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
Select
TRIM25 Rabbit anti-Human Polyclonal (aa325-375) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
TRIM25 Antibody - Western blot analysis of lysates from K562 cells, using ZNF147 Antibody. The lane on the right is blocked with the synthesized peptide.
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TRIM25 Rabbit anti-Human Polyclonal (aa201-250) Antibody
Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
TRIM25 Antibody - Western blot analysis of extracts of various cell lines, using TRIM25 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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TRIM25 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IP, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).