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TPX2

TPX2, microtubule-associated

Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.

Gene Name: TPX2, microtubule-associated
Synonyms: TPX2, C20orf2, DIL-2, DIL2, HCA519, GD:C20orf1, p100, Protein fls353, Targeting protein for Xklp2, C20orf1, FLS353, HCTP4, REPP86
Target Sequences: NM_012112 NP_036244.2 Q9ULW0

Publications (6)

1
Tumor cell dependence on Ran-GTP-directed mitosis. Xia F, Lee CW, Altieri DC. Cancer research. 2008 68:1826-33. [PubMed:18339863]
2
A survivin-ran complex regulates spindle formation in tumor cells. Xia F, Canovas PM, Guadagno TM, Altieri DC. Molecular and cellular biology. 2008 28:5299-311. [PubMed:18591255] [PMC:PMC2519733]
3
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A. Bird AW, Hyman AA. The Journal of cell biology. 2008 182:289-300. [PubMed:18663142] [PMC:PMC2483532]
4
Identification of a physiological E2 module for the human anaphase-promoting complex. Williamson A, Wickliffe KE, Mellone BG, Song L, Karpen GH, Rape M. Proceedings of the National Academy of Sciences of the United States of America. 2009 106:18213-8. [PubMed:19822757] [PMC:PMC2775311]
5
Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux. Ma N, Tulu US, Ferenz NP, Fagerstrom C, Wilde A, Wadsworth P. Molecular biology of the cell. 2010 21:979-88. (ICC; Pig) [PubMed:20110350] [PMC:PMC2836978]
6
Aurora B prevents delayed DNA replication and premature mitotic exit by repressing p21(Cip1). Trakala M, Fernández-Miranda G, Pérez de Castro I, Heeschen C, Malumbres M. Cell cycle (Georgetown, Tex.). 2013 12:1030-41. (WB; Mouse) [Full Text Article] [PubMed:23428904] [PMC:PMC3646860] Related Antibodies: LS-B146.
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Proteins (4)
Over-Expression Lysate (2)
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TPX2 (4)
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GST, N-terminus (1)
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HEK 293 Cells (2)
Wheat Germ Extract (1)
TPX2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
85.5 kDa
20 µg/$150
TPX2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
85.5 kDa
100 µg/$494
TPX2 Protein - Purified recombinant protein TPX2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
85.5 kDa
20 µg/$1,107
TPX2 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).