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TPSAB1 / Mast Cell Tryptase

Tryptase alpha/beta 1

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

Gene Name: Tryptase alpha/beta 1
Family/Subfamily: Protease , Serine S1
Synonyms: TPSAB1, Alpha tryptase, Mast cell alpha II tryptase, Tryptase alpha-1, Tryptase beta I, Tryptase-III, TPSB1, Tryptase alpha II, TPS1, TPS2, Tryptase beta-1, Tryptase-1, Tryptase-I, Mast cell beta I tryptase, Tryptase alpha/beta 1, Tryptase alpha/beta-1, Tryptase beta 1, Tryptase I
Target Sequences: NM_003294 NP_003285.2 Q15661

Publications (2)

1
PTH promotes allograft integration in a calvarial bone defect. Sheyn D, Cohn Yakubovich D, Kallai I, Su S, Da X, Pelled G, Tawackoli W, Cook-Weins G, Schwarz EM, Gazit D, Gazit Z. Molecular pharmaceutics. 2013 10:4462-71. (WB; Mouse) [Full Text Article] [PubMed:24131143] [PMC:PMC3902084] Related Antibodies: LS-C18207.
2
Benralizumab attenuates airway eosinophilia in prednisone-dependent asthma. Sehmi R, Lim HF, Mukherjee M, Huang C, Radford K, Newbold P, Boulet LP, Dorscheid D, Martin JG, Nair P. The Journal of allergy and clinical immunology. 2018 April;141:1529-1532 e8. [Full Text Article] [PubMed:29382593]

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Proteins (9)
Over-Expression Lysate (2)
Recombinant (7)
TPSAB1 / Mast Cell Tryptase (9)
No (9)
10His-SUMO, N-terminus + Myc, C-terminus (1)
6His, C-terminus (2)
GST (1)
His (2)
His,C-terminus (1)
Myc-DDK (Flag) (2)
Human (6)
Mouse (1)
Rat (1)
293T Cells (1)
E. coli (3)
HEK 293 Cells (1)
Human Cells (1)
Mammalian Cells (2)
Wheat Germ Extract (1)
Purified (5)
Low endotoxin level (4)
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Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
28.8kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,353
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Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
28.8 kDa
10 µg/$317; 50 µg/$524
TPSAB1 / Mast Cell Tryptase Protein
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E. coli
His
50 µg/$361; 250 µg/$739; 10 µg/$261
TPSAB1 / Mast Cell Tryptase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His-SUMO, N-terminus + Myc, C-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
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Wheat Germ Extract
GST
55.99 kDa
2 µg/$439
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Mammalian Cells
His,C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
29.3 kDa
10 µg/$539; 50 µg/$1,097
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E. coli
244 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
26.95 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
TPSAB1 / Mast Cell Tryptase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
30.53 kDa
20 µg/$150
TPSAB1 / Mast Cell Tryptase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
30.53 kDa
100 µg/$494
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).