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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TPMT

thiopurine S-methyltransferase

TPMT is the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X.

Gene Name: thiopurine S-methyltransferase
Synonyms: TPMT, Thiopurine S-methyltransferase, Thiopurine methyltransferase
Target Sequences: NM_000367 NP_000358.1 P51580

Filtered By: None
Target
TPMT (38)
Reactivity
Human (36)
Mouse (12)
Rat (2)
Dog (1)
Pig (4)
Application
IHC (20)
IHC-P (10)
WB (37)
Flo (1)
ELISA (15)
ICC (3)
IF (5)
Host
rabbit (32)
mouse (6)
Isotype
IgG (25)
IgG1 (4)
IgG1,k (2)
Clonality
monoclonal mc (6)
polyclonal pc (32)
Clone
1B5 (1)
2A2 (1)
4B1 (1)
4C1 (1)
5B4 (1)
AT2E7 (1)
Format
APC (1)
APC Conjugated (1)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (20)
Epitope
aa26-227 (10)
aa152-201 (4)
N-Terminus (1)
aa200-250 (1)
Publications
No (38)
Showing 1-25 of 38 results

TPMT Antibody IHC-plus™ LS-B10634
Rabbit Polyclonal (IgG) to Human TPMT
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $425.00
Anti-TPMT antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Thiopurine S-methyltransferase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa Additional bands at: 90 kDa, 100 kDa. We are unsure as to the identity of these extra bands.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TPMT antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Thiopurine S-methyltransferase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa Additional bands at: 90 kDa, 100 kDa. We are unsure as to the identity of these extra bands.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

TPMT Antibody (HRP) LS-C211221
Rabbit Polyclonal (IgG) to Human TPMT
Human
ELISA IHC IHC-P WB
HRP Conjugated
50µg  $285.00; 100µg  $385.00
Anti-TPMT antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Thiopurine S-methyltransferase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa Additional bands at: 90 kDa, 100 kDa. We are unsure as to the identity of these extra bands.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TPMT antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Thiopurine S-methyltransferase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa Additional bands at: 90 kDa, 100 kDa. We are unsure as to the identity of these extra bands.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

TPMT Antibody (Biotin) LS-C211223
Rabbit Polyclonal (IgG) to Human TPMT
Human
ELISA IHC IHC-P WB
Biotin Conjugated
50µg  $285.00; 100µg  $385.00
Anti-TPMT antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Thiopurine S-methyltransferase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa Additional bands at: 90 kDa, 100 kDa. We are unsure as to the identity of these extra bands.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TPMT antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Thiopurine S-methyltransferase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa Additional bands at: 90 kDa, 100 kDa. We are unsure as to the identity of these extra bands.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

TPMT Antibody (FITC) LS-C211224
Rabbit Polyclonal (IgG) to Human TPMT
Human
ELISA IHC IHC-P WB
FITC Conjugated
50µg  $285.00; 100µg  $385.00
Anti-TPMT antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Thiopurine S-methyltransferase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa Additional bands at: 90 kDa, 100 kDa. We are unsure as to the identity of these extra bands.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TPMT antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Thiopurine S-methyltransferase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa Additional bands at: 90 kDa, 100 kDa. We are unsure as to the identity of these extra bands.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

TPMT Antibody (clone 2A2) LS-C115018
Mouse Monoclonal [clone 2A2] (IgG1) to Human TPMT
Human
IF IHC IHC-P WB
Unconjugated
100µl  $335.00
IHC of paraffin-embedded breast tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).

TPMT Antibody (clone 1B5) LS-C133493
Mouse Monoclonal [clone 1B5] (IgG1,k) to Human TPMT
Human
ELISA IF IHC IHC-P WB
Unconjugated
100µg  $355.00
Immunoperoxidase of monoclonal antibody to TPMT on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1 ug/ml]
Immunofluorescence of monoclonal antibody to TPMT on HeLa cell. [antibody concentration 10 ug/ml]
Immunoperoxidase of monoclonal antibody to TPMT on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1 ug/ml]
Immunofluorescence of monoclonal antibody to TPMT on HeLa cell. [antibody concentration 10 ug/ml]

TPMT Antibody (clone 4C1) LS-C115096
Mouse Monoclonal [clone 4C1] (IgG1) to Human TPMT
Dog, Human
Flo IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of paraffin-embedded liver tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).

TPMT Antibody IHC-plus™ LS-B14110
Rabbit Polyclonal (IgG) to Human TPMT
ELISA IHC IHC-P WB
Unconjugated
40µg 80µl 0.5mg/ml  $425.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

TPMT Antibody (clone 5B4) LS-C115017
Mouse Monoclonal [clone 5B4] (IgG1) to Human TPMT
Human
IHC IHC-P WB
Unconjugated
100µl  $335.00
IHC of paraffin-embedded Kidney tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).

TPMT Antibody LS-C331205
Rabbit Polyclonal (IgG) to Human TPMT
Mouse, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of U2OS cells using TPMT antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of K562 cells, using TPMT antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Immunofluorescence analysis of U2OS cells using TPMT antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of K562 cells, using TPMT antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

TPMT Antibody (clone 4B1) LS-C115110
Mouse Monoclonal [clone 4B1] (IgG1) to Human TPMT
Human
IHC IHC-P WB
Unconjugated
100µl  $335.00
IHC of paraffin-embedded colon tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-TPMT mouse monoclonal antibody. (Dilution 1:50).

TPMT Antibody LS-C482248
Rabbit Polyclonal to Human TPMT
Mouse, Rat, Human
ICC IF WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $295.00; 200µl 1mg/ml  $375.00
Immunofluorescent analysis of TPMT staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of TPMT expression in K562 (A); mouse liver (B) whole cell lysates.
Immunofluorescent analysis of TPMT staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of TPMT expression in K562 (A); mouse liver (B) whole cell lysates.

TPMT Antibody LS-C749080
Rabbit Polyclonal (IgG) to Human TPMT
Mouse, Human
IF WB
Unconjugated
50µl  $235.00

TPMT Antibody LS-C192514
Rabbit Polyclonal to Human TPMT
Mouse, Rat, Human
IHC WB
Unconjugated
100µl  $385.00
Western blot of TPMT pAb in extracts from K562 cells and mouse liver, kidney tissues.
Western blot of TPMT pAb in extracts from K562 cells and mouse liver, kidney tissues.

TPMT Antibody (aa26-227) LS-C373026
Rabbit Polyclonal (IgG) to Human TPMT
Mouse, Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00; 10ml 0.5mg/ml  $2,100.00
Western blot of TPMT antibody.
Western blot of TPMT antibody.

TPMT Antibody (aa26-227, Biotin) LS-C422713
Rabbit Polyclonal (IgG) to Human TPMT
Human
ICC WB
Biotin Conjugated
100µl 0.5mg/ml  $375.00

TPMT Antibody (aa26-227, Biotin) LS-C687481
Rabbit Polyclonal (IgG) to Human TPMT
Mouse, Human
IHC WB
Biotin Conjugated
20µl  $205.00; 100µl  $265.00; 200µl  $315.00; 1ml  $575.00; 10ml  $2,310.00
Western blot of TPMT antibody.
Western blot of TPMT antibody.

TPMT Antibody (aa26-227, FITC) LS-C422712
Rabbit Polyclonal (IgG) to Human TPMT
Human
ICC WB
FITC Conjugated
100µl 0.5mg/ml  $395.00

TPMT Antibody (aa26-227, FITC) LS-C694308
Rabbit Polyclonal (IgG) to Human TPMT
Mouse, Human
IHC WB
FITC Conjugated
20µl  $215.00; 100µl  $275.00; 200µl  $325.00; 1ml  $635.00; 10ml  $2,520.00
Western blot of TPMT antibody.
Western blot of TPMT antibody.

TPMT Antibody (clone AT2E7) LS-C175796
Mouse Monoclonal [clone AT2E7] (IgG1,k) to Human TPMT
Human
ELISA WB
Unconjugated
50µl 1mg/ml  $315.00
Western Blot: The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

TPMT Antibody LS-C190041
Rabbit Polyclonal (IgG) to Human TPMT
Human
ELISA WB
Unconjugated
100µg  $455.00

TPMT Antibody (N-Terminus) LS-C92740
Rabbit Polyclonal (IgG) to Human TPMT
Human
ELISA WB
Unconjugated
40µg 0.5mg/ml  $405.00

TPMT Antibody (aa200-250) LS-C92741
Rabbit Polyclonal (IgG) to Human TPMT
Human
ELISA WB
Unconjugated
40µg 0.5mg/ml  $405.00

TPMT Antibody LS-C203085
Rabbit Polyclonal (IgG) to Human TPMT
ELISA WB
Unconjugated
40µg 80µl 0.5mg/ml  $245.00
Western blot of recombinant human TPMT protein using 1 ug/ml Rabbit Anti-TPMT Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Western blot of recombinant human TPMT protein using 1 ug/ml Rabbit Anti-TPMT Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

TPMT Antibody LS-C662485
Rabbit Polyclonal to Human TPMT
Human
ELISA WB
Unconjugated
100µg  $305.00
Western blot - Anti-TPMT antibody
Western blot - Anti-TPMT antibody
Showing 1-25 of 38 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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