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Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Gene Name: | topoisomerase (DNA) III beta |
Family/Subfamily: | Topoisomerase |
Synonyms: | TOP3B, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, Topoisomerase (DNA) III beta, TOP3B1, Topoisomerase III beta |
Target Sequences: | NM_003935 NP_003926.1 O95985 |
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