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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TOP2B / Topoisomerase II Beta

topoisomerase (DNA) II beta 180kDa

TOP2B / Topoisomerase II Beta is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described.

Gene Name: topoisomerase (DNA) II beta 180kDa
Family/Subfamily: Topoisomerase
Synonyms: TOP2B, DNA topoisomerase II beta, DNA topoisomerase 2-beta, Topo II beta, Antigen MLAA-44, TOPIIB, Topoisomerase II beta, Topoisomerase IIb, U937 associated antigen, DNA topoisomerase II, 180 kD, Top2beta
Target Sequences: NM_001068 NP_001059.2 Q02880

Viewing 1-25 of 34 items
TOP2B / Topoisomerase II Beta Antibody - Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Dog, Pig, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
TOP2B / Topoisomerase II Beta Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100µg   $435.00
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Mouse, Human
ELISA, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Mouse, Human
ELISA, IHC, WB
Unconjugated
100µg   $305.00
TOP2B / Topoisomerase II Beta Antibody - Immunofluorescence staining of TOP2B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TOP2B polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ICC, IF
Unconjugated
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
TOP2B / Topoisomerase II Beta Antibody - Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
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Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
IHC, IP, WB
Unconjugated
100µl   0.2mg/ml   $405.00
TOP2B / Topoisomerase II Beta Antibody - Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
IHC, IP, WB
Unconjugated
100µl   0.2mg/ml   $405.00
TOP2B / Topoisomerase II Beta Antibody - Immunohistochemistry image of paraffin-embedded human cervical cancer at a dilution of 1:100
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ELISA, IHC, IHC-P
Unconjugated
50µg   $285.00; 100µg   $385.00
TOP2B / Topoisomerase II Beta Antibody - Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TOP2B Antibody. The picture on the right is blocked with the synthesized peptide.
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Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Mouse, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
TOP2B / Topoisomerase II Beta Antibody - IHC of Topoisomerase II (A32) pAb in paraffin-embedded human lung carcinoma tissue.
Select
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl   $345.00
TOP2B / Topoisomerase II Beta Antibody
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ELISA, IHC
Unconjugated
100µl   1mg/ml   $265.00
TOP2B / Topoisomerase II Beta Antibody
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ELISA, WB
Unconjugated
100µl   1mg/ml   $265.00
TOP2B / Topoisomerase II Beta Antibody - Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:250.
Select
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
IHC
Unconjugated
100µl   $415.00
TOP2B / Topoisomerase II Beta Antibody - Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
WB
Unconjugated
100µl   0.5mg/ml   $265.00; 200µl   0.5mg/ml   $305.00; 500µl   0.5mg/ml   $445.00; 1ml   0.5mg/ml   $545.00
TOP2B / Topoisomerase II Beta Antibody - Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
WB
HRP Conjugated
200µl   0.5mg/ml   $345.00; 500µl   0.5mg/ml   $560.00; 1ml   0.5mg/ml   $695.00
TOP2B / Topoisomerase II Beta Antibody - Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
WB
Biotin Conjugated
200µl   0.5mg/ml   $315.00; 500µl   0.5mg/ml   $475.00; 1ml   0.5mg/ml   $590.00
TOP2B / Topoisomerase II Beta Antibody - Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
WB
FITC Conjugated
200µl   0.5mg/ml   $365.00; 500µl   0.5mg/ml   $605.00; 1ml   0.5mg/ml   $755.00
TOP2B / Topoisomerase II Beta Antibody - Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
WB
APC, Cy7 Conjugated
1ml   0.1mg/ml   $1,215.00
TOP2B / Topoisomerase II Beta Antibody - Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
WB
Cy3 Conjugated
200µl   0.1mg/ml   $445.00; 500µl   0.1mg/ml   $860.00; 1ml   0.1mg/ml   $1,080.00
TOP2B / Topoisomerase II Beta Antibody - Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
WB
APC Conjugated
200µl   0.1mg/ml   $375.00; 500µl   0.1mg/ml   $650.00; 1ml   0.1mg/ml   $815.00
TOP2B / Topoisomerase II Beta Antibody - Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
WB
PE Conjugated
200µl   0.1mg/ml   $485.00; 500µl   0.1mg/ml   $965.00; 1ml   0.1mg/ml   $1,215.00
TOP2B / Topoisomerase II Beta Antibody - TOP2B antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Rabbit, Mouse, Dog, Guinea pig, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
TOP2B / Topoisomerase II Beta Antibody - TOP2B antibody Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Rabbit, Mouse, Dog, Guinea pig, Rat, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
TOP2B / Topoisomerase II Beta Antibody - Anti-Topoisomerase 2 beta Antibody at 1:1000 dilution + MOLT-4 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 183 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Select
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Human
WB
Unconjugated
100µl   $325.00

Viewing 1-25 of 34 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).