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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TOP2B / Topoisomerase II Beta

topoisomerase (DNA) II beta 180kDa

TOP2B / Topoisomerase II Beta is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described.

Gene Name: topoisomerase (DNA) II beta 180kDa
Family/Subfamily: Topoisomerase
Synonyms: TOP2B, DNA topoisomerase II beta, DNA topoisomerase 2-beta, Topo II beta, Antigen MLAA-44, TOPIIB, Topoisomerase II beta, Topoisomerase IIb, U937 associated antigen, DNA topoisomerase II, 180 kD, Top2beta
Target Sequences: NM_001068 NP_001059.2 Q02880

Filtered by: None
Target
TOP2B / Topoisomerase II Beta (34)
Reactivity
Human (32)
Mouse (22)
Rat (8)
Bat (4)
Chimpanzee (6)
Dog (9)
Gibbon (6)
Guinea pig (8)
Horse (8)
Monkey (9)
Orangutan (6)
Pig (9)
Rabbit (8)
Application
IHC (18)
IHC-P (6)
WB (28)
ELISA (6)
ICC (1)
IF (5)
IP (3)
Peptide-ELISA (1)
Host
rabbit (34)
Isotype
IgG (13)
Clonality
polyclonal pc (34)
Format
APC (1)
APC Conjugated (1)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (16)
Epitope
aa446-655 (10)
aa1439-1488 (4)
aa1548-1597 (4)
C-Terminus (3)
10-90 aa, N-terminal (1)
Ala32 (1)
N-Terminus (1)
aa1-50 (1)
aa1475-1525 (1)
Publications
No (34)
Showing 1-25 of 34 results

TOP2B / Topoisomerase II Beta Antibody (N-Terminus) LS-C352951
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Dog, Pig, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Topoisomerase 2 beta staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

TOP2B / Topoisomerase II Beta Antibody LS-C661171
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ELISA IF IHC IP WB
Unconjugated
100µg  $435.00

TOP2B / Topoisomerase II Beta Antibody (aa1475-1525) LS-C287783
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
IF IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

TOP2B / Topoisomerase II Beta Antibody (C-Terminus) LS-C287782
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
IF IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

TOP2B / Topoisomerase II Beta Antibody (aa446-655) LS-C296718
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00; 10ml 0.5mg/ml  $2,160.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

TOP2B / Topoisomerase II Beta Antibody (aa446-655, HRP) LS-C712757
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
IHC WB
HRP Conjugated
100µl 0.5mg/ml  $295.00; 200µl 0.5mg/ml  $345.00; 1ml 0.5mg/ml  $695.00; 10ml 0.5mg/ml  $2,805.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

TOP2B / Topoisomerase II Beta Antibody (aa446-655, Biotin) LS-C688103
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
IHC WB
Biotin Conjugated
100µl 0.5mg/ml  $275.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $590.00; 10ml 0.5mg/ml  $2,370.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

TOP2B / Topoisomerase II Beta Antibody (aa446-655, Biotin) LS-C299481
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse
WB
Biotin Conjugated
100µl 0.5mg/ml  $375.00
Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

TOP2B / Topoisomerase II Beta Antibody (aa446-655, FITC) LS-C695018
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
IHC WB
FITC Conjugated
100µl 0.5mg/ml  $285.00; 200µl 0.5mg/ml  $335.00; 1ml 0.5mg/ml  $650.00; 10ml 0.5mg/ml  $2,580.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

TOP2B / Topoisomerase II Beta Antibody (aa446-655, FITC) LS-C302977
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse
WB
FITC Conjugated
100µl 0.5mg/ml  $395.00
Western blot of recombinant TOP2B / Topoisomerase II Beta.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

TOP2B / Topoisomerase II Beta Antibody (aa446-655, Cy3) LS-C703735
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
IHC WB
Cy3 Conjugated
100µl 0.5mg/ml  $365.00; 200µl 0.5mg/ml  $445.00; 1ml 0.5mg/ml  $1,080.00; 10ml 0.5mg/ml  $4,320.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

TOP2B / Topoisomerase II Beta Antibody (aa446-655, APC) LS-C718445
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
IHC WB
APC Conjugated
100µl  $315.00; 200µl  $375.00; 1ml  $815.00; 10ml  $3,930.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

TOP2B / Topoisomerase II Beta Antibody (aa446-655, PE) LS-C726820
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
IHC WB
PE Conjugated
100µl  $285.00; 200µl  $335.00; 1ml  $650.00; 10ml  $2,580.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

TOP2B / Topoisomerase II Beta Antibody (aa446-655, APC, Cy7) LS-C735742
Rabbit Polyclonal to Mouse TOP2B / Topoisomerase II Beta
Mouse, Human
IHC WB
APC, Cy7 Conjugated
100µl  $465.00; 200µl  $650.00; 1ml  $1,620.00; 10ml  $6,480.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

TOP2B / Topoisomerase II Beta Antibody (C-Terminus) LS-C285926
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
IF IHC IHC-P
Unconjugated
100µl  $415.00
Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:250.
Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:250.

TOP2B / Topoisomerase II Beta Antibody (10-90 aa, N-terminal) LS-C386465
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Mouse, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00

TOP2B / Topoisomerase II Beta Antibody LS-C670070
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ELISA IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image of paraffin-embedded human cervical cancer at a dilution of 1:100

TOP2B / Topoisomerase II Beta Antibody (Ala32) LS-C176355
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Mouse, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of Topoisomerase II (A32) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of Topoisomerase II (A32) pAb in extracts from Jurkat cells.

TOP2B / Topoisomerase II Beta Antibody (aa1-50) LS-C118622
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Mouse, Human
IHC Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TOP2B Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using TOP2B Antibody. The lane on the right is blocked with the synthesized peptide.

TOP2B / Topoisomerase II Beta Antibody (C-Terminus) LS-C137150
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ELISA IHC
Unconjugated
100µl  $275.00
Immunohistochemistry Image

TOP2B / Topoisomerase II Beta Antibody LS-C406899
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Mouse, Human
ELISA WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Western blot analysis of Raw264.7, Jurkat, K562 and hepg2 cell, using TOP2B Polyclonal Antibody at dilution of 1:250.

TOP2B / Topoisomerase II Beta Antibody LS-C136868
Rabbit Polyclonal (IgG) to Human TOP2B / Topoisomerase II Beta
Human
ELISA WB
Unconjugated
100µl  $275.00
Western blot Image

TOP2B / Topoisomerase II Beta Antibody LS-C357013
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Human
WB
Unconjugated
100µl  $325.00
Anti-Topoisomerase 2 beta Antibody at 1:1000 dilution + MOLT-4 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 183 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

TOP2B / Topoisomerase II Beta Antibody (aa1439-1488) LS-C109740
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Rabbit, Mouse, Dog, Guinea pig, Rat, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
TOP2B antibody Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

TOP2B / Topoisomerase II Beta Antibody (aa1548-1597) LS-C110235
Rabbit Polyclonal to Human TOP2B / Topoisomerase II Beta
Rabbit, Mouse, Dog, Guinea pig, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
TOP2B antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 34 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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