TOP1 / Topoisomerase I

topoisomerase (DNA) I

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

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Products

Proteins (5)

Type

Over-Expression Lysate (2)

Recombinant (3)

Target

TOP1 / Topoisomerase I (5)

Publications

No (5)

Tag

ABP-His, N-terminus (1)

GST, N-terminus (1)

His-T7 (1)

Myc-DDK (Flag) (2)

Species

Human (4)

Source

293T Cells (1)

E. coli (2)

HEK 293 Cells (1)

Wheat Germ Extract (1)

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Human TOP1 / Topoisomerase I Protein (Recombinant His-T7) (aa1-250) - LS-G14851

Source:

E. coli

Tag:

His-T7

Molecular Weight:

34.0 kDa

Price:

10 µg/$283; 50 µg/$452; 100 µg/$722; 200 µg/$902; 1 mg/$2,016; 500 µg/$1,553; 5 mg/$3,151; 2 mg/$2,204

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Human TOP1 / Topoisomerase I Protein (Recombinant GST, N-terminus) (aa692-765) - LS-G60398

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human TOP1 / Topoisomerase I Protein (Recombinant ABP-His, N-terminus) - LS-G145281

Source:

E. coli

Tag:

ABP-His, N-terminus

Molecular Weight:

21.9 kDa

Price:

50 µg/$419

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Human TOP1 / Topoisomerase I Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G85590

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

90.5 kDa

Price:

20 µg/$215

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TOP1 / Topoisomerase I Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G100016

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

90.5 kDa

Price:

100 µg/$710

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