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THEMIS / SPOT

thymocyte selection associated

Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.

Gene Name: thymocyte selection associated
Synonyms: THEMIS, C6orf207, C6orf190, BA325O24.3, GRB2-associated protein, Protein THEMIS, SPOT, Thymocyte selection associated, BA325O24.4, TSEPA, GASP
Target Sequences: NP_848781.1 Q8BGW0

☰ Filters
Antibodies (19)
Proteins (3)
ELISA Kits (2)
Over-Expression Lysate (2)
Primary (19)
Recombinant (1)
Sandwich (2)
THEMIS / SPOT (24)
Human (17)
Mouse (11)
Rat (1)
Gibbon (3)
Monkey (3)
IHC (7)
IHC-P (1)
WB (16)
Flo (1)
ELISA (7)
IF (1)
IP (1)
Peptide-ELISA (1)
rabbit (16)
goat (1)
rat (1)
sheep (1)
IgG (12)
IgG2b (1)
monoclonal mc (1)
polyclonal pc (18)
12K186 (1)
96-Well Microplate (2)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (8)
aa559-588 (7)
C-Terminus (4)
aa122-237 (2)
aa577-590 (1)
aa591-641 (1)
No (24)
Cell Lysates (2)
Tissue Homogenates (2)
GST (1)
Myc-DDK (Flag) (2)
Human (2)
293T Cells (1)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (1)
THEMIS / SPOT Antibody - THEMIS Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human thymoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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THEMIS / SPOT Rabbit anti-Human Polyclonal (aa559-588) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
THEMIS / SPOT Antibody - THEMIS antibody (0.3 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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THEMIS / SPOT Goat anti-Mouse Polyclonal (aa577-590) Antibody
Mouse
Peptide-ELISA, WB
Unconjugated
100 µg/$503
THEMIS / SPOT Antibody - IHC image of THEMIS Antibody diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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THEMIS / SPOT Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, WB
Unconjugated
50 µg/$294; 100 µg/$360
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (aa559-588) Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (aa559-588) (Biotin) Antibody
Mouse, Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (aa559-588) (FITC) Antibody
Mouse, Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (aa559-588) (APC) Antibody
Mouse, Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (aa559-588) (PE) Antibody
Mouse, Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (aa559-588) (Azide-free) (HRP) Antibody
Mouse, Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
Select
THEMIS / SPOT Rat anti-Mouse Monoclonal (aa122-237) (12K186) Antibody
Mouse
Flo, WB
Unconjugated
100 µg/$856
THEMIS / SPOT Antibody - Detection of human THEMIS by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THEMIS antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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THEMIS / SPOT Rabbit anti-Human Polyclonal (aa591-641) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
Select
THEMIS / SPOT Sheep anti-Mouse Polyclonal (aa122-237) Antibody
Mouse, Rat
WB
Unconjugated
100 µg/$919
THEMIS / SPOT Antibody - THEMIS antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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THEMIS / SPOT Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
THEMIS / SPOT Antibody - THEMIS antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (C-Terminus) (HRP) Antibody
Human, Monkey, Gibbon
WB
HRP Conjugated
100 µl/$469
THEMIS / SPOT Antibody - THEMIS antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (C-Terminus) (Biotin) Antibody
Human, Monkey, Gibbon
WB
Biotin Conjugated
100 µl/$469
THEMIS / SPOT Antibody - THEMIS antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
THEMIS / SPOT Rabbit anti-Human Polyclonal (C-Terminus) (FITC) Antibody
Human, Monkey, Gibbon
WB
FITC Conjugated
100 µl/$469
THEMIS / SPOT ELISA Kit
Select
Sandwich
96-Well Microplate
Mouse
0.312 - 20 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Tissue Homogenates
1 Plate/$923
THEMIS / SPOT ELISA Kit
Select
Sandwich
96-Well Microplate
Human
0.312 - 20 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Tissue Homogenates
1 Plate/$902
THEMIS / SPOT Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
77.3 kDa
20 µg/$215
THEMIS / SPOT Protein - 12.5% SDS-PAGE of human THEMIS stained with Coomassie Blue
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Wheat Germ Extract
GST
97 kDa
10 µg/$479; 25 µg/$670
THEMIS / SPOT Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
77.3 kDa
100 µg/$710
Viewing 1-24 of 24 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).