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TDG / Thymine DNA Glycosylase

thymine-DNA glycosylase

DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

Gene Name: thymine-DNA glycosylase
Synonyms: TDG, Thymine-DNA glycosylase, Thymine DNA Glycosylase
Target Sequences: NM_003211 NP_003202.3 Q13569

Filtered By: None
Target
TDG / Thymine DNA Glycosylase (16)
Reactivity
Human (15)
Mouse (12)
Rat (8)
Bovine (1)
Monkey (1)
Application
IHC (5)
IHC-P (1)
WB (16)
Flo (2)
ELISA (5)
ICC (1)
IF (5)
IP (2)
Peptide-ELISA (1)
Host
rabbit (15)
mouse (1)
Isotype
IgG (9)
IgG1,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (15)
Clone
AT2F7 (1)
Format
Unconjugated (16)
Epitope
aa141-410 (2)
1-80 aa, Internal (1)
Internal (1)
Lys90 (1)
aa281-310 (1)
aa31-80 (1)
aa360-410 (1)
aa81-110 (1)
Publications
No (16)
Showing 1-16 of 16 results

TDG / Thymine DNA Glycosylase Antibody (Internal) LS-C354097
Rabbit Polyclonal to Human TDG / Thymine DNA Glycosylase
Mouse, Bovine, Rat, Human
ICC IF WB
Unconjugated
100µl 1mg/ml  $245.00
Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Thymine DNA Glycosylase expression in HeLa (A); mouse lung (B); rat lung (C) whole cell lysates.
Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Thymine DNA Glycosylase expression in HeLa (A); mouse lung (B); rat lung (C) whole cell lysates.

TDG / Thymine DNA Glycosylase Antibody (1-80 aa, Internal) LS-C386361
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

TDG / Thymine DNA Glycosylase Antibody (aa141-410) LS-C377570
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Mouse, Human
ELISA IHC IHC-P WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human heart using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human heart using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.

TDG / Thymine DNA Glycosylase Antibody LS-C334266
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Mouse, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of A549 cells.
Immunofluorescence analysis of A549 cells.
Immunofluorescence analysis of A549 cells.
Immunofluorescence analysis of A549 cells.

TDG / Thymine DNA Glycosylase Antibody (Lys90) LS-C176808
Rabbit Polyclonal to Human TDG / Thymine DNA Glycosylase
Mouse, Rat, Human
IF IHC WB
Unconjugated
100µl  $345.00
Western blot of TDG (K90) pAb in extracts from Jurkat cells.
Western blot of TDG (K90) pAb in extracts from Jurkat cells.

TDG / Thymine DNA Glycosylase Antibody LS-C748418
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Mouse, Rat, Human
IF WB
Unconjugated
50µl  $235.00
Immunofluorescence analysis of A549 cells using TDG antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using TDG antibody. Blue: DAPI for nuclear staining.

TDG / Thymine DNA Glycosylase Antibody LS-C660952
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Human
ELISA IP WB
Unconjugated
100µg  $435.00

TDG / Thymine DNA Glycosylase Antibody LS-C305941
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Mouse, Rat, Human
IHC WB
Unconjugated
100µg 1mg/ml  $495.00
Western blot of TDG / Thymine DNA Glycosylase antibody LS-X223194.
Western blot of TDG / Thymine DNA Glycosylase antibody LS-X223194.

TDG / Thymine DNA Glycosylase Antibody (clone AT2F7) LS-C200834
Mouse Monoclonal [clone AT2F7] (IgG1,k) to Human TDG / Thymine DNA Glycosylase
Human
ELISA WB
Unconjugated
50µl 1mg/ml  $315.00
Western Blot: The cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: The cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

TDG / Thymine DNA Glycosylase Antibody (aa281-310) LS-C99358
Rabbit Polyclonal to Human TDG / Thymine DNA Glycosylase
Human
Flo WB
Unconjugated
400µl  $305.00
Western blot of TDG antibody in K562 cell line lysates (35 ug/lane). TDG (arrow) was detected using the purified antibody.
Flow cytometric of K562 cells using TDG Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot of TDG antibody in K562 cell line lysates (35 ug/lane). TDG (arrow) was detected using the purified antibody.
Flow cytometric of K562 cells using TDG Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TDG / Thymine DNA Glycosylase Antibody (aa81-110) LS-C99357
Rabbit Polyclonal to Human TDG / Thymine DNA Glycosylase
Mouse, Human
Flo WB
Unconjugated
400µl  $305.00
Western blot of TDG antibody in mouse lung tissue lysates (35 ug/lane). TDG (arrow) was detected using the purified antibody.
TDG Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot of TDG antibody in mouse lung tissue lysates (35 ug/lane). TDG (arrow) was detected using the purified antibody.
TDG Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TDG / Thymine DNA Glycosylase Antibody (aa141-410) LS-C377568
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Mouse, Human
ELISA WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Western blot. All lanes: G/T mismatch-specific thymine DNA glycosylase antibody at 8 ug/ml+mouse brain tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa.
Western blot. All lanes: G/T mismatch-specific thymine DNA glycosylase antibody at 8 ug/ml+mouse brain tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa.

TDG / Thymine DNA Glycosylase Antibody (aa31-80) LS-C119026
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Mouse, Rat, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from Jurkat cells, using TDG Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using TDG Antibody. The lane on the right is blocked with the synthesized peptide.

TDG / Thymine DNA Glycosylase Antibody (aa360-410) LS-C291004
Rabbit Polyclonal to Human TDG / Thymine DNA Glycosylase
Human, Monkey
IP WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human TDG by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TDG antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human TDG by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TDG antibody used for IP at 6 ug per reaction. TDG was also immunoprecipitated by rabbit anti-TDG antibody BL16331. For blotting immunoprecipitated TDG, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human TDG by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TDG antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human TDG by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TDG antibody used for IP at 6 ug per reaction. TDG was also immunoprecipitated by rabbit anti-TDG antibody BL16331. For blotting immunoprecipitated TDG, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

TDG / Thymine DNA Glycosylase Antibody LS-C417034
Rabbit Polyclonal to Human TDG / Thymine DNA Glycosylase
Mouse, Rat, Human
WB
Unconjugated
100µg 1mg/ml  $305.00

TDG / Thymine DNA Glycosylase Antibody LS-C335140
Rabbit Polyclonal (IgG) to Human TDG / Thymine DNA Glycosylase
Mouse, Rat
WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of mouse brain, using TDG antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of mouse brain, using TDG antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Showing 1-16 of 16 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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