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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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TEK / TIE2

TEK tyrosine kinase, endothelial

Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.

Gene Name: TEK tyrosine kinase, endothelial
Family/Subfamily: Protein Kinase , Tie/Tek
Synonyms: TEK, Angiopoietin-1 receptor, CD202b antigen, CD202B, Endothelial tyrosine kinase, HTIE2, Hyk, Soluble TIE2 variant 1, Soluble TIE2 variant 2, TIE-2, TIE2, VMCM, Tek tyrosine kinase, VMCM1, p140 TEK
Target Sequences: NM_000459 NP_000450.2 Q02763

Publications (2)

1
VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury. Ohkubo H, Ito Y, Minamino T, Eshima K, Kojo K, Okizaki S, Hirata M, Shibuya M, Watanabe M, Majima M. PloS one. 2014 9:e105533. (WB, Flo; Mouse) [Full Text Article] [PubMed:25162491] [PMC:PMC4146544]
2
Vasculogenic and hematopoietic cellular progenitors are scattered within the prenatal mouse heart. Jankowska-Steifer E, Madej M, Niderla-Bielinska J, Ruminski S, Flaht-Zabost A, Czarnowska E, Gula G, Radomska-Lesniewska DM, Ratajska A. Histochemistry and cell biology. 2015 143:153-69. (WB; Mouse) [Full Text Article] [PubMed:25201347] [PMC:PMC4298664]

☰ Filters
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TEK / TIE2 Rabbit anti-Human Polyclonal (pTyr1100) Antibody
Human
IHC, IHC-Fr, IHC-P, WB
Unconjugated
100 µg/$665
TEK / TIE2 Antibody - TEK monoclonal antibody (M20), clone 3A5. Western blot of TEK expression in PC-12.
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TEK / TIE2 Mouse anti-Human Monoclonal (3A5) Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$365
TEK / TIE2 ELISA Kit
Select
Cell-Based Phosphorylation
96-Well Microplate
Mouse, Human
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
1 Plate/$590
TEK / TIE2 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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TEK / TIE2 Rabbit anti-Human Polyclonal (aa758-789) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$325
TEK / TIE2 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
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TEK / TIE2 Rabbit anti-Human Polyclonal Antibody
Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$425
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TEK / TIE2 Rabbit anti-Human Polyclonal (pTyr1108) Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µl/$345
TEK / TIE2 ELISA Kit
Select
Cell-Based
96-Well Microplate
Mouse, Human
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
1 Plate/$490
TEK / TIE2 Protein - Recombinant TEK Tyrosine Kinase, Endothelial By SDS-PAGE
Select
E. coli
His-GST
63.6 kDa
10 µg/$285; 50 µg/$485; 100 µg/$650; 200 µg/$965; 1 mg/$2,460
TEK / TIE2 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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TEK / TIE2 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$425
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TEK / TIE2 Rat anti-Mouse Monoclonal (TEK4) Antibody
Mouse
Flo, IF
Unconjugated
25 µg/$225
TEK / TIE2 Antibody - 1:100 staining human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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TEK / TIE2 Rabbit anti-Human Polyclonal (pTyr992) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100 µl/$325; 200 µl/$375
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TEK / TIE2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$755
TEK / TIE2 Antibody - Western blot analysis of TIE2 (pY1108) expression in MCF7 PMA-treated (A); HUVEC (B); mouse brain (C); rat brain (D) whole cell lysates.
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TEK / TIE2 Rabbit anti-Human Polyclonal (pTyr1108) Antibody
Zebrafish, Rat, Mouse, Bovine, Human
WB
Unconjugated
100 µl/$265
TEK / TIE2 Antibody - Western blot analysis of TIE2 expression in HUVEC (A); HepG2 (B) whole cell lysates.
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TEK / TIE2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Zebrafish, Mouse, Bovine, Human
WB
Unconjugated
30 µl/$205; 100 µl/$245; 200 µl/$305
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TEK / TIE2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, Flo, ICC, IF
Unconjugated
50 µl/$245; 100 µl/$305; 1 ml/$1,335
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TEK / TIE2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$355
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TEK / TIE2 Mouse anti-Human Monoclonal Antibody
Human
Flo, ICC, IF
Unconjugated
50 µl/$235; 100 µl/$275; 200 µl/$305
TEK / TIE2 Antibody - Western blot analysis using CD202B mAb against HEK293 (1) and CD202B (AA: 23-745)-hIgGFc transfected HEK293 (2) cell lysate.
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TEK / TIE2 Mouse anti-Human Monoclonal (3F5B9) Antibody
Human
ELISA, Flo, WB
Unconjugated
100 µg/$365
TEK / TIE2 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Rat
0.312 - 20 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$780
TEK / TIE2 Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using TEK antibody at dilution of 1:100 (40x lens).
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TEK / TIE2 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$215; 50 µl/$235; 100 µl/$295; 200 µl/$385
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TEK / TIE2 Rabbit anti-Human Polyclonal (1050-1130 aa) Antibody
Mouse, Human
ELISA, IF, WB
Unconjugated
50 µl/$235; 100 µl/$265; 200 µl/$335
Select
TEK / TIE2 Rabbit anti-Human Polyclonal (aa758-789) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$590
TEK / TIE2 Antibody - Western blot analysis of extracts from NIH-3T3 cells, using TIE2 (Phospho-Tyr1108) antibody.
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TEK / TIE2 Rabbit anti-Human Polyclonal (pTyr1108) Antibody
Mouse, Human
ELISA, IF, WB
Unconjugated
100 µg/$305
TEK / TIE2 Antibody - 1:100 staining human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
TEK / TIE2 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100 µl/$325; 200 µl/$375
Select
TEK / TIE2 Rabbit anti-Human Polyclonal (1040-1120 aa) Antibody
Mouse, Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$235; 100 µl/$265; 200 µl/$335
Viewing 1-25 of 165 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).