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SUMO1 / SMT3

small ubiquitin-like modifier 1

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.

Gene Name: small ubiquitin-like modifier 1
Synonyms: SUMO1, GAP modifying protein 1, GMP1, OFC10, Sentrin, SMT3 homolog 3, SMT3C, SMT3H3, SUMO-1, Ubiquitin-like protein UBL1, PIC1, Ubiquitin-like 1 (sentrin), UBL1, DAP1, GAP-modifying protein 1, Ubiquitin-like protein SMT3C
Target Sequences: NM_003352 NP_003343.1 P63165

Publications (4)

1
SUMO modification of the Ets-related transcription factor ERM inhibits its transcriptional activity. Degerny C, Monte D, Beaudoin C, Jaffray E, Portois L, Hay RT, de Launoit Y, Baert JL. The Journal of biological chemistry. 2005 280:24330-8. [PubMed:15857832] Related Antibodies: LS-C161612.
2
SUMOylation attenuates the aggregation propensity and cellular toxicity of the polyglutamine expanded ataxin-7. Janer A, Werner A, Takahashi-Fujigasaki J, Daret A, Fujigasaki H, Takada K, Duyckaerts C, Brice A, Dejean A, Sittler A. Human molecular genetics. 2010 19:181-95. [PubMed:19843541]
3
Insights into high affinity small ubiquitin-like modifier (SUMO) recognition by SUMO-interacting motifs (SIMs) revealed by a combination of NMR and peptide array analysis. Namanja AT, Li YJ, Su Y, Wong S, Lu J, Colson LT, Wu C, Li SS, Chen Y. The Journal of biological chemistry. 2012 287:3231-40. [PubMed:22147707] [PMC:PMC3270977] Related Antibodies: LS-C96460.
4
Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila. Rathke C, Baarends WM, Jayaramaiah-Raja S, Bartkuhn M, Renkawitz R, Renkawitz-Pohl R. Journal of cell science. 2007 120:1689-700. [PubMed:17452629] Related Antibodies: LS-C98764.
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Proteins (13)
Over-Expression Lysate (5)
Recombinant (8)
SUMO1 / SMT3 (13)
No (13)
6His, N-terminus (2)
GST (1)
His (1)
His, N-Terminal (1)
His,N-terminus (1)
His-T7 (1)
Myc-DDK (Flag) (5)
Human (11)
293T Cells (3)
E. coli (7)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (4)
Low endotoxin level (3)
SUMO1 / SMT3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
11.4 kDa
20 µg/$150
Select
293T Cells
Myc-DDK (Flag)
11.4 kDa
20 µg/$150
SUMO1 / SMT3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
11.4 kDa
20 µg/$150
SUMO1 / SMT3 Protein - 12.5% SDS-PAGE of human SUMO1 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
38 kDa
10 µg/$479; 25 µg/$670
SUMO1 / SMT3 Protein - Recombinant  Small Ubiquitin Related Modifier Protein 1 By SDS-PAGE
Select
E. coli
His-T7
15.3 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
13.7kD
10 µg/$218; 50 µg/$233; 500 µg/$355; 1 mg/$416
SUMO1 / SMT3 Protein
Select
E. coli
His, N-Terminal
11.4 kDa
50 µg/$419
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
13.7 kDa
10 µg/$220; 50 µg/$233
Select
E. coli
His,N-terminus
12.4 kDa
50 µg/$721
SUMO1 / SMT3 Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
SUMO1 / SMT3 Protein
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$345; 500 µg/$657; 20 µg/$254
SUMO1 / SMT3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
11.4 kDa
100 µg/$494
SUMO1 / SMT3 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
11.4 kDa
100 µg/$494
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).