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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPRY2 / Sprouty 2

sprouty homolog 2 (Drosophila)

SPRY2 / Sprouty 2 is a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.

Gene Name: sprouty homolog 2 (Drosophila)
Synonyms: SPRY2, HSPRY2, Protein sprouty homolog 2, Sprouty homolog 2 (Drosophila), Sprouty 2, Spry-2, Sprouty (Drosophila) homolog 2
Target Sequences: NM_005842 NP_005833.1 O43597

Publications (1)

1
Regulation of vascular smooth muscle cell proliferation and migration by human sprouty 2. Zhang C, Chaturvedi D, Jaggar L, Magnuson D, Lee JM, Patel TB. Arteriosclerosis, thrombosis, and vascular biology. 2005 25:533-8. [PubMed:15653567]

Filtered by: None
Target
SPRY2 / Sprouty 2 (63)
Reactivity
Human (55)
Mouse (37)
Rat (24)
Bovine (4)
Chimpanzee (6)
Dog (9)
Gibbon (6)
Hamster (9)
Horse (4)
Monkey (4)
Orangutan (6)
Pig (9)
Rabbit (4)
Application
IHC (10)
IHC-P (6)
WB (58)
ELISA (17)
ICC (1)
IF (5)
IP (4)
Host
rabbit (61)
mouse (1)
goat (1)
Isotype
IgG (31)
Clonality
monoclonal mc (1)
polyclonal pc (62)
Clone
1E10 (1)
Format
AP Conjugated (2)
APC (3)
APC Conjugated (5)
Azide-free (1)
Biotin Conjugated (8)
Cy3 Conjugated (3)
Cy7 Conjugated (3)
FITC Conjugated (8)
HRP Conjugated (8)
PE Conjugated (5)
Unconjugated (21)
Epitope
aa116-305 (16)
aa116-304 (8)
aa6-35 (8)
N-Terminus (4)
aa107-156 (4)
aa1-175 (1)
aa26-39 (1)
aa265-315 (1)
aa75-125 (1)
Publications
No (63)
Showing 1-25 of 63 results

IHC-plus™ SPRY2 / Sprouty 2 Antibody LS-B10618
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $485.00
Anti-SPRY2 / Sprouty 2 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SPRY2 / Sprouty 2 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

SPRY2 / Sprouty 2 Antibody (clone 1E10) LS-C133632
Mouse Monoclonal [clone 1E10] (IgG) to Human SPRY2 / Sprouty 2
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
100µg  $365.00
Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml].
Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25 ug/ml]

SPRY2 / Sprouty 2 Antibody LS-C499867
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µg  $285.00; 100µg 4mg/ml  $385.00
Immunohistochemistry of paraffin-embedded human brain tissue using SPRY2 Antibody at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using SPRY2 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IHC-plus™ SPRY2 / Sprouty 2 Antibody (aa107-156) LS-B5819
Rabbit Polyclonal to Human SPRY2 / Sprouty 2
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl  $485.00
Anti-SPRY2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPRY2 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPRY2 / Sprouty 2 Antibody (aa107-156, HRP) LS-C453847
Rabbit Polyclonal to Human SPRY2 / Sprouty 2
Rabbit, Mouse, Dog, Bovine, Pig, Horse, Rat, Hamster, Chimpanzee, Orangutan, Gibbon, Human, Monkey
IHC, IHC-P, WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Anti-SPRY2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPRY2 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPRY2 / Sprouty 2 Antibody (aa107-156, Biotin) LS-C453845
Rabbit Polyclonal to Human SPRY2 / Sprouty 2
Rabbit, Mouse, Dog, Bovine, Pig, Horse, Rat, Hamster, Chimpanzee, Orangutan, Gibbon, Human, Monkey
IHC, IHC-P, WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Anti-SPRY2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPRY2 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPRY2 / Sprouty 2 Antibody (aa107-156, FITC) LS-C453846
Rabbit Polyclonal to Human SPRY2 / Sprouty 2
Rabbit, Mouse, Dog, Bovine, Pig, Horse, Rat, Hamster, Chimpanzee, Orangutan, Gibbon, Human, Monkey
IHC, IHC-P, WB
FITC Conjugated
100µl 1mg/ml  $385.00
Anti-SPRY2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPRY2 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPRY2 / Sprouty 2 Antibody (aa116-305) LS-C296500
Rabbit Polyclonal to Mouse SPRY2 / Sprouty 2
Mouse
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $275.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $590.00
Western blot of recombinant SPRY2 / Sprouty 2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot; Sample: Mouse Heart lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse SPRY2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

SPRY2 / Sprouty 2 Antibody LS-C745356
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Human
ELISA, IF, IHC, WB
Unconjugated
25µl 1.04mg/ml  $235.00
Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.

SPRY2 / Sprouty 2 Antibody LS-C755355
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Human
ELISA, IF, IHC, WB
Unconjugated
100µg  $355.00

SPRY2 / Sprouty 2 Antibody (aa116-305) LS-C296498
Rabbit Polyclonal to Human SPRY2 / Sprouty 2
Human
ICC, IP, WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $575.00
Western blot of recombinant SPRY2 / Sprouty 2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot; Sample: Human Lung lysate; Primary Ab: 3µg/ml Rabbit Anti-Human SPRY2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

SPRY2 / Sprouty 2 Antibody LS-C10336
Rabbit Polyclonal to Human SPRY2 / Sprouty 2
Human
ELISA, IP, WB
Unconjugated
100µg  $755.00

SPRY2 / Sprouty 2 Antibody LS-C412300
Rabbit Polyclonal to Human SPRY2 / Sprouty 2
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl  $385.00

SPRY2 / Sprouty 2 Antibody (aa6-35) LS-C330177
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, WB
Unconjugated
200µl  $590.00

SPRY2 / Sprouty 2 Antibody (aa6-35, Biotin) LS-C270258
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

SPRY2 / Sprouty 2 Antibody (aa6-35, FITC) LS-C270259
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, WB
FITC Conjugated
200µl  $740.00

SPRY2 / Sprouty 2 Antibody (aa6-35, AP) LS-C270256
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, WB
AP Conjugated
200µl  $740.00

SPRY2 / Sprouty 2 Antibody (aa6-35, Azide-free, HRP) LS-C270260
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

SPRY2 / Sprouty 2 Antibody (aa6-35, PE) LS-C270261
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, WB
PE Conjugated
200µl  $740.00

SPRY2 / Sprouty 2 Antibody (aa6-35, APC) LS-C270257
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, WB
APC Conjugated
200µl  $740.00

SPRY2 / Sprouty 2 Antibody LS-C778999
Rabbit Polyclonal to Human SPRY2 / Sprouty 2
Mouse, Human
ELISA, WB
Unconjugated
100µg 100µl 1mg/ml  $305.00

SPRY2 / Sprouty 2 Antibody (aa26-39) LS-C10337
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Dog, Rat, Hamster, Pig, Human
WB
Unconjugated
200µg  $605.00

SPRY2 / Sprouty 2 Antibody (N-Terminus) LS-C81563
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Dog, Rat, Hamster, Pig, Human
WB
Unconjugated
100µl 0.5mg/ml  $385.00
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPRY2 / Sprouty 2 Antibody LS-C334596
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
WB
Unconjugated
100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of A375 cells.

SPRY2 / Sprouty 2 Antibody LS-C746797
Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Mouse, Human
WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines, using SPRY2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
Showing 1-25 of 63 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).