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SPP1 / Osteopontin
secreted phosphoprotein 1
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
| Gene Name: | secreted phosphoprotein 1 |
| Synonyms: | SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I |
| Target Sequences: | NM_000582 NP_000573.1 P10451 |
Publications (8)
1
Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences. Kon S, Yokosaki Y, Maeda M, Segawa T, Horikoshi Y, Tsukagoshi H, Rashid MM, Morimoto J, Inobe M, Shijubo N, Chambers AF, Uede T. Journal of cellular biochemistry. 2002 84:420-32.
[PubMed:11787071]
2
Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells. Robertson BW, Chellaiah MA. Experimental cell research. 2010 316:11-Jan.
[PubMed:19850036]
[PMC:PMC2787770]
Related Antibodies: LS-B425.
3
Expression of chondro-osteogenic BMPs in clinical samples of patellar tendinopathy. Rui YF, Lui PP, Rolf CG, Wong YM, Lee YW, Chan KM. Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA. 2012 20:1409-17. (IHC; Human)
[PubMed:21946950]
☰ Filters
Products
Proteins
(33)
Type
Over-Expression Cell Culture Supernatant
(1)
Over-Expression Lysate
(7)
Recombinant
(25)
Target
SPP1 / Osteopontin
(33)
Publications
No
(33)
Tag
6His, C-terminus
(2)
6His, N-terminus
(2)
GST
(1)
GST, N-terminus
(2)
His
(7)
His, N-Terminal
(4)
His-GST
(1)
Myc-DDK (Flag)
(9)
Species
Dog
(1)
Human
(23)
Mouse
(3)
Rat
(2)
Rhesus monkey
(1)
Source
293F Cells
(2)
293T Cells
(3)
E. coli
(6)
HEK 293 Cells
(13)
Human
(3)
Human Cells
(1)
Mammalian Cells
(1)
Wheat Germ Extract
(3)
Yeast
(1)
Purification
Purified
(2)
Low Endotoxin Level
Low endotoxin level
(6)
Biologically Active
Biologically active
(1)
HEK 293 Cells
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
60.0-65.0 kDa
10 µg/$268; 50 µg/$349
E. coli
His
34.8 kDa
10 µg/$284; 50 µg/$462; 100 µg/$737; 200 µg/$922; 1 mg/$2,042; 500 µg/$1,577; 5 mg/$3,221; 2 mg/$2,231
Yeast
His
32.3 kD
100 µg/$499; 20 µg/$364; 1 mg/$1,683
HEK 293 Cells
6His, N-terminus
55-65 kDa
100 µg/$298
E. coli
His
26.6 kDa
10 µg/$291; 50 µg/$502; 100 µg/$802; 200 µg/$1,002; 1 mg/$2,144; 500 µg/$1,668; 5 mg/$3,501; 2 mg/$2,334
HEK 293 Cells
Myc-DDK (Flag)
33.84 kDa
100 µg/$494
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
293T Cells
Myc-DDK (Flag)
33.84 kDa
20 µg/$150
293T Cells
Myc-DDK (Flag)
35.42 kDa
20 µg/$150
293T Cells
Myc-DDK (Flag)
32.36 kDa
20 µg/$150
HEK 293 Cells
Myc-DDK (Flag)
35.42 kDa
100 µg/$494
Wheat Germ Extract
GST
58.74 kDa
10 µg/$479; 25 µg/$670
HEK 293 Cells
His
32.3 kDa
10 µg/$392; 50 µg/$782; 100 µg/$1,133
HEK 293 Cells
His
34.9 kDa
10 µg/$377; 50 µg/$752; 100 µg/$1,099
HEK 293 Cells
6His, N-terminus
55-65 kDa
200 µl/$283
Human
None
50 µg/$882; 10 µg/$352; 200 µg/$1,529; 100 µg/$1,303; 1 mg/$2,845; 500 µg/$2,341; 5 mg/$6,161; 2 mg/$3,287
Human
None
32.4kDa
50 µg/$742; 10 µg/$329; 200 µg/$1,352; 100 µg/$1,143; 1 mg/$2,631; 500 µg/$2,127; 5 mg/$5,181; 2 mg/$2,822
Human
None
50 µg/$305; 10 µg/$248; 200 µg/$472; 100 µg/$380; 1 mg/$1,308; 500 µg/$945; 5 mg/$2,183; 2 mg/$1,462
HEK 293 Cells
Myc-DDK (Flag)
33.7 kDa
20 µg/$1,107
HEK 293 Cells
Myc-DDK (Flag)
32.1 kDa
20 µg/$1,107
HEK 293 Cells
Myc-DDK (Flag)
30.6 kDa
20 µg/$1,107
HEK 293 Cells
Myc-DDK (Flag)
32.36 kDa
100 µg/$494
E. coli
106 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
11.77 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
E. coli
94 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
10.45 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
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