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SOD2 / Mn SOD

superoxide dismutase 2, mitochondrial

MNSOD / SOD2 is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene Name: superoxide dismutase 2, mitochondrial
Synonyms: SOD2, IPOB, MVCD6, Indophenoloxidase B, Mn superoxide dismutase, MNSOD, Mangano-superoxide dismutase
Target Sequences: NM_000636 NP_000627.2 P04179

Publications (4)

1
A novel Drosophila SOD2 mutant demonstrates a role for mitochondrial ROS in neurodevelopment and disease. Celotto AM, Liu Z, Vandemark AP, Palladino MJ. Brain and behavior. 2012 2:424-34. (WB; Rat) [Full Text Article] [PubMed:22950046] [PMC:PMC3432965] Related Antibodies: LS-B3694.
2
Production of functional classical brown adipocytes from human pluripotent stem cells using specific hemopoietin cocktail without gene transfer. Nishio M, Yoneshiro T, Nakahara M, Suzuki S, Saeki K, Hasegawa M, Kawai Y, Akutsu H, Umezawa A, Yasuda K, Tobe K, Yuo A, Kubota K, Saito M, Saeki K. Cell metabolism. 2012 16:394-406. (IHC; Human) [Full Text Article] [PubMed:22958922]
3
Monosodium luminol reinstates redox homeostasis, improves cognition, mood and neurogenesis, and alleviates neuro- and systemic inflammation in a model of Gulf War Illness. Shetty AK, Attaluri S, Kodali M, Shuai B, Shetty GA, Upadhya D, Hattiangady B, Madhu LN, Upadhya R, Bates A, Rao X. Redox biology. 2020 January;28: [Full Text Article] [PubMed:31778892] [PMC:PMC6888767]
4
Differential structural remodeling of the left-atrial posterior wall in patients affected by mitral regurgitation with or without persistent atrial fibrillation: a morphological and molecular study. Domenico Corradi, Sergio Callegari, Roberta Maestri, David Ferrara, Domenica Mangieri, Rossella Alinovi, Paola Mozzoni, Silvana Pinelli, Matteo Goldoni, Ylenia Adelaide Privitera, Veronica Bartoli, Ettore Astorri, Emilio Macchi, Augusto Vaglio, Stefano Benussi, Ottavio Alfieri. Journal of cardiovascular electrophysiology. 2012 March;23:271-9. [Full Text Article] [PubMed:21954878]
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Proteins (26)
Over-Expression Lysate (6)
Recombinant (19)
SOD2 / Mn SOD (26)
No (26)
6His, C-terminus (2)
6His, N-terminus (2)
GST (3)
GST, N-terminus (1)
His (7)
His-T7 (1)
Myc-DDK (Flag) (8)
Alternaria alternata (1)
Human (18)
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Rat (2)
293T Cells (3)
E. coli (13)
HEK 293 Cells (5)
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Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
23.2 kDa
10 µg/$368; 50 µg/$479
SOD2 / Mn SOD Protein
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E. coli
His
Biologically active
100 µg/$342; 500 µg/$641; 20 µg/$254
SOD2 / Mn SOD Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
SOD2 / Mn SOD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
20.72 kDa
100 µg/$494
SOD2 / Mn SOD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
24.75 kDa
100 µg/$494
SOD2 / Mn SOD Protein - SDS-PAGE of 25kDa rat Mn SOD.
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E. coli
His
50 µg/$538; 100 µg/$995
SOD2 / Mn SOD Protein - SDS-PAGE of 25kDa human Mn SOD.
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E. coli
His
50 µg/$538; 100 µg/$995
SOD2 / Mn SOD Protein - 12.5% SDS-PAGE of human SOD2 stained with Coomassie Blue
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Wheat Germ Extract
GST
49.94 kDa
10 µg/$479; 25 µg/$670
SOD2 / Mn SOD Protein - 12.5% SDS-PAGE of human SOD2 stained with Coomassie Blue
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Wheat Germ Extract
GST
41.14 kDa
10 µg/$479; 25 µg/$670
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E. coli
GST
30.1 kD
1 mg/$1,679; 100 µg/$598; 20 µg/$405
SOD2 / Mn SOD Protein - Purified recombinant protein SOD2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
22.2 kDa
20 µg/$1,107
SOD2 / Mn SOD Protein - Purified recombinant protein SOD2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
22.2 kDa
20 µg/$1,107
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293T Cells
Myc-DDK (Flag)
24.75 kDa
20 µg/$150
SOD2 / Mn SOD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
24.75 kDa
20 µg/$150
SOD2 / Mn SOD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
20.72 kDa
20 µg/$150
SOD2 / Mn SOD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
24.75 kDa
100 µg/$494
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E. coli
None
22.3 kDa
100 µg/$721
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E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
23.7 kDa
10 µg/$344; 50 µg/$584
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E. coli
None
24.4 kDa
5 µg/$329; 25 µg/$412; 1000 µg/$3,015
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E. coli
221 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
24.42 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
SOD2 / Mn SOD Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
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E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
23.7kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,157
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Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
23.24kD
10 µg/$281; 50 µg/$395; 500 µg/$1,585; 1 mg/$2,157
SOD2 / Mn SOD Protein - Recombinant Superoxide Dismutase 2, Mitochondrial By SDS-PAGE
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E. coli
His
23.5 kDa
10 µg/$255; 50 µg/$323; 100 µg/$450; 200 µg/$562; 1 mg/$1,480; 500 µg/$1,096; 5 mg/$2,395; 2 mg/$1,646
Select
E. coli
His
23.5 kDa
10 µg/$262; 50 µg/$339; 100 µg/$512; 200 µg/$642; 1 mg/$1,623; 500 µg/$1,214; 5 mg/$2,562; 2 mg/$1,796
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).