SMAD3

SMAD family member 3

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

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Products

ELISA Kits (24)

Type

Cell-Based (1)

Cell-Based Phosphorylation (5)

Custom (5)

DNA Binding (1)

DNA Binding Phosphorylation (1)

Sandwich (10)

Sandwich CLIA (1)

Target

SMAD3 (24)

Reactivity

Human (14)

Mouse (10)

Rat (12)

Chicken (2)

Monkey (1)

Pig (1)

Porcine (1)

Rabbit (1)

Format

12 x 8-Well Microstrips (3)

96-Well Microplate (9)

96-Well Strip Plate (12)

Publications

No (23)

Yes (1)

Sample Type

Adherent Cell Cultures (6)

Cell Lysates (4)

Nuclear Lysates (2)

Plasma (6)

Serum (6)

Tissue Homogenates (4)

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Mouse/Human/Rat SMAD3 (Sandwich ELISA) ELISA Kit - LS-F6417

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse, Human, Rat

Range:

0.625 - 40 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$854

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Mouse/Human/Rat Phospho-SMAD3 (Ser213) (DNA-Binding Phosphorylation ELISA) ELISA Kit - LS-F926

Type:

DNA Binding Phosphorylation

Format:

12 x 8-Well Microstrips

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Nuclear Lysates

Price:

1 Plate/$735

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Mouse/Human/Rat Phospho-SMAD3 (Ser204) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1376

Type:

Cell-Based Phosphorylation

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$668

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Human SMAD3 (Custom ELISA) ELISA Kit - LS-F50727

Type:

Custom

Format:

96-Well Microplate

Reactivity:

Human

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$672

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Chicken SMAD3 (Sandwich ELISA) ELISA Kit - LS-F45794

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Chicken

Range:

0.625 - 40 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Monkey SMAD3 (Sandwich ELISA) ELISA Kit - LS-F45795

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Monkey

Range:

0.625 - 40 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

Select

Porcine SMAD3 (Sandwich ELISA) ELISA Kit - LS-F45796

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Porcine

Range:

0.625 - 40 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Rabbit SMAD3 (Sandwich ELISA) ELISA Kit - LS-F55242

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rabbit

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$893

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Rat SMAD3 (Sandwich ELISA) ELISA Kit - LS-F56203

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rat

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum, Tissue Homogenates

Price:

1 Plate/$579

Select

Human SMAD3 (Sandwich ELISA) ELISA Kit - LS-F22584

Type:

Sandwich

Format:

96-Well Microplate

Reactivity:

Human

Range:

0.625 - 40 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$651

Select

Rat SMAD3 (Sandwich ELISA) ELISA Kit - LS-F21581

(4.0) Rating

(1) Publication(s)

Type:

Sandwich

Format:

96-Well Microplate

Reactivity:

Rat

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$651

Select

Chicken SMAD3 (Custom ELISA) ELISA Kit - LS-F19437

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Chicken

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$929

Select

Rat SMAD3 (Custom ELISA) ELISA Kit - LS-F19438

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Rat

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$783

Select

Mouse SMAD3 (Custom ELISA) ELISA Kit - LS-F19439

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Mouse

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$783

Select

Pig SMAD3 (Custom ELISA) ELISA Kit - LS-F19440

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Pig

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$783

Select

Human SMAD3 (Sandwich ELISA) ELISA Kit - LS-F12616

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.625 - 40 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$783

Select

Human SMAD3 (CLIA) ELISA Kit - LS-F29857

Type:

Sandwich CLIA

Format:

12 x 8-Well Microstrips

Reactivity:

Human

Range:

0.156 - 10 ng/ml

Detection:

Chemiluminescent

Sample Types:

Plasma, Serum

Price:

1 Plate/$728

Select

Human SMAD3 (Sandwich ELISA) ELISA Kit - LS-F37229

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.625 - 40 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum, Tissue Homogenates

Price:

1 Plate/$579

Select

Mouse/Human/Rat Phospho-SMAD3 (Thr179) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1375

Type:

Cell-Based Phosphorylation

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$668

Select

Mouse/Human/Rat Phospho-SMAD3 (Ser208) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1377

Type:

Cell-Based Phosphorylation

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$668

Select

Mouse/Human/Rat Phospho-SMAD3 (Ser213) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1378

Type:

Cell-Based Phosphorylation

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$668

Select

Mouse/Human/Rat SMAD3 (Cell-Based ELISA) ELISA Kit - LS-F2314

Type:

Cell-Based

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$618

Select

Mouse/Human/Rat SMAD3 (DNA-Binding ELISA) ELISA Kit - LS-F821

Type:

DNA Binding

Format:

12 x 8-Well Microstrips

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Nuclear Lysates

Price:

1 Plate/$694

Select

Mouse/Human/Rat Phospho-SMAD3 (Ser425) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1058

Type:

Cell-Based Phosphorylation

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$668

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