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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SART1

squamous cell carcinoma antigen recognized by T cells

SART1 is two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.

Gene Name: squamous cell carcinoma antigen recognized by T cells
Synonyms: SART1, Ara1, Allergen Hom s 1, HOMS1, HSnu66, IgE autoantigen, SART-1, Snu66, SNRNP110, SNU66 homolog, SART1(800) protein, SART1259, HSART-1, SART1(259) protein
Target Sequences: NM_005146 NP_005137.1 O43290

Filtered By: None
Target
SART1 (9)
Reactivity
Human (9)
Mouse (5)
Rat (5)
Bat (1)
Bovine (3)
Dog (2)
Guinea pig (2)
Horse (2)
Monkey (1)
Pig (2)
Rabbit (1)
Zebrafish (1)
Application
IHC (1)
IHC-P (1)
WB (8)
ELISA (1)
ICC (1)
IF (1)
IP (2)
Peptide-ELISA (1)
Host
rabbit (8)
goat (1)
Isotype
IgG (6)
Clonality
polyclonal pc (9)
Format
Unconjugated (9)
Epitope
aa137-447 (1)
aa2-14 (1)
aa533-792 (1)
aa675-725 (1)
aa750-800 (1)
Publications
No (9)
Showing 1-9 of 9 results

SART1 Antibody (aa137-447) LS-C185540
Rabbit Polyclonal (IgG) to Human SART1
Human
ICC IHC IHC-P WB
Unconjugated
100µl 0.76mg/ml  $335.00
IHC of paraffin-embedded Cal27 xenograft using SART1 antibody at 1:500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using SART1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
IHC of paraffin-embedded Cal27 xenograft using SART1 antibody at 1:500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using SART1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

SART1 Antibody LS-C752952
Rabbit Polyclonal (IgG) to Human SART1
Human
ELISA IF WB
Unconjugated
100µg  $375.00

SART1 Antibody LS-C410104
Rabbit Polyclonal (IgG) to Human SART1
Mouse, Rat, Human
IP WB
Unconjugated
50µl  $255.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of various cell lines.
Immunoprecipitation analysis of 150ug extracts of Jurkat cells.
Western blot analysis of extracts of various cell lines.
Immunoprecipitation analysis of 150ug extracts of Jurkat cells.

SART1 Antibody (aa2-14) LS-C55435
Goat Polyclonal to Human SART1
Rabbit, Mouse, Bovine, Rat, Pig, Bat, Human, Monkey
Peptide-ELISA WB
Unconjugated
100µg 0.5mg/ml  $405.00
Antibody (0.03 ug/ml) staining of Hela lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.03 ug/ml) staining of Hela lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SART1 Antibody (aa750-800) LS-C288217
Rabbit Polyclonal (IgG) to Human SART1
Zebrafish, Horse, Rat, Mouse, Dog, Guinea pig, Bovine, Human
WB
Unconjugated
100µl 0.2mg/ml  $395.00

SART1 Antibody LS-C658827
Rabbit Polyclonal to Human SART1
Mouse, Rat, Human
WB
Unconjugated
100µl  $305.00

SART1 Antibody (aa533-792) LS-C186192
Rabbit Polyclonal (IgG) to Human SART1
Human
WB
Unconjugated
100µl 1mg/ml  $335.00
Sample (30 ug of whole cell lysate). A: H1299, B: Hela, C: Molt-4 . 7.5% SDS PAGE. SART1 antibody diluted at 1:5000.
Sample (30 ug of whole cell lysate). A: H1299, B: Hela, C: Molt-4 . 7.5% SDS PAGE. SART1 antibody diluted at 1:5000.

SART1 Antibody LS-C668106
Rabbit Polyclonal to Human SART1
Human
WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $275.00
Western blot Image
Western blot Image

SART1 Antibody (aa675-725) LS-C288215
Rabbit Polyclonal (IgG) to Human SART1
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Human
IP
Unconjugated
100µl 1mg/ml  $395.00
Showing 1-9 of 9 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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