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SAG / Arrestin

S-antigen; retina and pineal gland (arrestin)

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness.

Gene Name: S-antigen; retina and pineal gland (arrestin)
Synonyms: SAG, 48 kDa protein, Arrestin 1, ARRESTIN, S-arrestin, S-AG, Retinal S-antigen, Rod photoreceptor arrestin, S-antigen, RP47
Target Sequences: NM_000541 NP_000532.2 P10523

Publications (1)

1
Immunoaffinity purification of S-antigen using monoclonal antibodies to different antigenic sites. Banga JP, Suleyman S, Kasp E, Brown E, LeRoy F, Sanders M, Dumonde D. Investigative ophthalmology & visual science. 1987 28:604-7. [PubMed:3549614]

☰ Filters
Antibodies (38)
ELISA Kits (13)
Proteins (6)
Competitive EIA (2)
Custom (5)
Over-Expression Lysate (2)
Primary (38)
Recombinant (4)
Sandwich (6)
SAG / Arrestin (57)
Human (32)
Mouse (14)
Rat (5)
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Chicken (1)
Dog (1)
Monkey (1)
Pig (10)
Porcine (1)
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IHC-P (1)
WB (33)
ELISA (7)
IF (7)
Peptide-ELISA (2)
rabbit (27)
mouse (11)
Product Group
IHCPlus (1)
IgG (8)
IgG1 (9)
IgG1,k (1)
monoclonal mc (11)
polyclonal pc (27)
OTI7A12 (2)
PDS-1 (7)
S128 (1)
96-Well Strip Plate (13)
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DY550 Conjugated (1)
DY650 Conjugated (1)
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aa139-388 (8)
aa4-249 (8)
aa273-307 (1)
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Yeast (1)
SAG / Arrestin Antibody - Confocal image of a pig retina stained with SAG / Arrestin antibody (green). Visual arrestin is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL), and can be used to identify components of rod photoreceptor cells. (Cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The red stain is Fox2, an RNA binding nuclear protein related to Fox3/NeuN, which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).
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SAG / Arrestin Mouse anti-Human Monoclonal (S128) Antibody
Mouse, Bovine, Rat, Pig, Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$460
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SAG / Arrestin Mouse anti-Pig Monoclonal (DY650) (PDS-1) Antibody
Bovine, Pig, Human
IF, IHC, IHC-Fr, WB
DY650 Conjugated
0.1 ml/$416
SAG / Arrestin ELISA Kit
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Competitive EIA
96-Well Strip Plate
Human
250 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$913
SAG / Arrestin Antibody - Visual Arrestin (PDS-1): Western blot of Visual Arrestin expression in human retina tissue extract.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SAG / Arrestin Mouse anti-Human Monoclonal (PDS-1) Antibody
Bovine, Pig, Human
IF, WB
Unconjugated
0.1 ml/$386
SAG / Arrestin Antibody - Visual Arrestin (PDS-1): Western blot of Visual Arrestin expression in human retina tissue extract.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SAG / Arrestin Mouse anti-Pig Monoclonal (Biotin) (PDS-1) Antibody
Bovine, Pig, Human
IF, IHC, IHC-Fr, WB
Biotin Conjugated
0.1 ml/$416
SAG / Arrestin Antibody - Visual Arrestin (PDS-1): Western blot of Visual Arrestin expression in human retina tissue extract.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SAG / Arrestin Mouse anti-Pig Monoclonal (DY488) (PDS-1) Antibody
Bovine, Pig, Human
IF, IHC, IHC-Fr, WB
DY488 Conjugated
0.1 ml/$416
SAG / Arrestin Antibody - Visual Arrestin (PDS-1): Western blot of Visual Arrestin expression in human retina tissue extract.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SAG / Arrestin Mouse anti-Pig Monoclonal (DY550) (PDS-1) Antibody
Bovine, Pig, Human
IF, IHC, IHC-Fr, WB
DY550 Conjugated
0.1 ml/$416
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SAG / Arrestin Mouse anti-Pig Monoclonal (PDS-1) Antibody
Bovine, Pig, Human
IHC, WB
Unconjugated
0.1 ml/$482
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SAG / Arrestin Mouse anti-Pig Monoclonal Antibody
Bovine, Pig, Human
IF, IHC, IHC-Fr, WB
Unconjugated
100 µl/$649
SAG / Arrestin ELISA Kit
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Sandwich
96-Well Strip Plate
Rat
0.312 - 20 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
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SAG / Arrestin Mouse anti-Pig Monoclonal (PDS-1) Antibody
Bovine, Pig, Human
IHC, IHC-Fr, WB
Unconjugated
50 µl/$429
SAG / Arrestin Antibody - 1:100 staining rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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SAG / Arrestin Rabbit anti-Human Polyclonal Antibody
Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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SAG / Arrestin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$341
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SAG / Arrestin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$357
SAG / Arrestin Antibody - Western blot analysis of Retinal S antigen using anti-Retinal S antigen antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye ball tissue lysates,Lane 2: mouse eye ball tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Retinal S antigen antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Retinal S antigen at approximately 45-55KD. The expected band size for Retinal S antigen is at 45KD.
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SAG / Arrestin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
10 µg/$318; 100 µg/$470
SAG / Arrestin Antibody - Anti-SAG Antibody (C-Term) at 1:2000 dilution + mouse eyeball lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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SAG / Arrestin Rabbit anti-Human Polyclonal (aa273-307) Antibody
Mouse
Peptide-ELISA, WB
Unconjugated
200 µl/$393
SAG / Arrestin Antibody - Immunohistochemistry of paraffin-embedded Mouse retina using SAG Polyclonal Antibody at dilution of 1:200 (40x lens).
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SAG / Arrestin Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
SAG / Arrestin Antibody - Western blot of SAG / Arrestin antibody.
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SAG / Arrestin Rabbit anti-Mouse Polyclonal (aa4-249) Antibody
Mouse
WB
Unconjugated
100 µl/$306; 200 µl/$347; 500 µl/$484; 1 ml/$553; 2 ml/$737; 5 ml/$1,206
SAG / Arrestin Antibody - Western blot of SAG / Arrestin antibody.
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SAG / Arrestin Rabbit anti-Mouse Polyclonal (aa4-249) (HRP) Antibody
Mouse
WB
HRP Conjugated
100 µl/$335; 200 µl/$389; 500 µl/$566; 1 ml/$685; 2 ml/$922; 5 ml/$1,557
SAG / Arrestin Antibody - Western blot of SAG / Arrestin antibody.
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SAG / Arrestin Rabbit anti-Mouse Polyclonal (aa4-249) (FITC) Antibody
Mouse
WB
FITC Conjugated
100 µl/$345; 200 µl/$402; 500 µl/$594; 1 ml/$737; 2 ml/$976; 5 ml/$1,676
SAG / Arrestin Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY SAG. (Right lane) cDNA for 48 hrs and lysed
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SAG / Arrestin Mouse anti-Human Monoclonal (OTI7A12) Antibody
Human
WB
Unconjugated
100 µl/$434
SAG / Arrestin Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY SAG. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAG. (1:500)
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SAG / Arrestin Mouse anti-Human Monoclonal (Carrier-free) (OTI7A12) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
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SAG / Arrestin Rabbit anti-Human Polyclonal Antibody
Human
ELISA
Unconjugated
50 µg/$294; 100 µg/$360
SAG / Arrestin Antibody - Western blot of recombinant SAG / Arrestin.
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SAG / Arrestin Rabbit anti-Human Polyclonal (aa139-388) Antibody
Human
WB
Unconjugated
100 µl/$303; 200 µl/$343; 500 µl/$476; 1 ml/$543; 2 ml/$715; 5 ml/$1,182
SAG / Arrestin Antibody - Western blot analysis of extracts of various cell lines, using SAG antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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SAG / Arrestin Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 100 µl/$389
Viewing 1-25 of 57 product results



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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).