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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RUNX1T1 / ETO

runt-related transcription factor 1; translocated to, 1 (cyclin D-related)

RUNX1T1 / ETO is a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.

Gene Name: runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Synonyms: RUNX1T1, AML1T1, CBFA2T1, CDR, Eight twenty one protein, Cyclin-D-related protein, ETO, MTG8, Protein CBFA2T1, Protein ETO, ZMYND2, Protein MTG8
Target Sequences: NM_004349 NP_004340.1 Q06455

Filtered by: None
Target
RUNX1T1 / ETO (28)
Reactivity
Human (28)
Mouse (12)
Rat (14)
Bat (8)
Bovine (4)
Chicken (8)
Chimpanzee (3)
Dog (12)
Gibbon (9)
Guinea pig (8)
Horse (8)
Monkey (12)
Pig (12)
Rabbit (12)
Zebrafish (8)
Application
IHC (8)
IHC-P (3)
WB (21)
ChrIP (1)
ELISA (11)
IF (3)
IP (1)
Host
rabbit (25)
mouse (3)
Isotype
IgG (15)
IgG1 (2)
IgG2a,k (1)
Clonality
monoclonal mc (3)
polyclonal pc (25)
Clone
5A12 (1)
OTI1H1 (2)
Format
Biotin Conjugated (4)
Carrier-free (1)
FITC Conjugated (4)
HRP Conjugated (4)
Unconjugated (16)
Epitope
N-Terminus (6)
aa221-270 (4)
aa288-337 (4)
aa554-604 (1)
Publications
No (28)
Showing 1-25 of 28 results

IHC-plus™ RUNX1T1 / ETO Antibody (aa554-604) LS-B13526
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human
IHC, IHC-P, IP, WB
Unconjugated
50µl 1mg/ml  $425.00
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

RUNX1T1 / ETO Antibody LS-C403498
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Mouse, Human
ELISA, IHC
Unconjugated
20µl 0.5mg/ml  $205.00; 60µl 0.5mg/ml  $235.00; 120µl 0.5mg/ml  $295.00; 200µl 0.5mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human thyroid cancer using RUNX1T1 Polyclonal Antibody at dilution of 1:35.

RUNX1T1 / ETO Antibody (aa288-337) LS-C112160
Rabbit Polyclonal to Human RUNX1T1 / ETO
Rabbit, Dog, Rat, Pig, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $385.00
RUNX1T1 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody (aa221-270) LS-C31287
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Chicken, Zebrafish, Pig, Horse, Bat, Rabbit, Mouse, Dog, Guinea pig, Rat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $325.00
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody (aa221-270, HRP) LS-C430422
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Chicken, Zebrafish, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $335.00
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody (aa221-270, Biotin) LS-C430420
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Chicken, Zebrafish, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Gibbon, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $335.00
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody (aa221-270, FITC) LS-C430421
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Chicken, Zebrafish, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $335.00
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody LS-C662563
Rabbit Polyclonal to Human RUNX1T1 / ETO
Human
ELISA, IHC, IHC-P, WB
Unconjugated
100µg  $305.00
Immunohistochemistry - Anti-RUNX1T1/ETO antibody
Immunohistochemistry - Anti-RUNX1T1/ETO antibody

RUNX1T1 / ETO Antibody (clone 5A12) LS-C196824
Mouse Monoclonal [clone 5A12] (IgG2a,k) to Human RUNX1T1 / ETO
Human
ELISA, IF, WB
Unconjugated
100µg  $365.00
Immunofluorescence of monoclonal antibody to RUNX1T1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.Lane 1: RUNX1T1 transfected lysate(67.566 KDa).Lane 2: Non-transfected lysate.

RUNX1T1 / ETO Antibody LS-C679377
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human pancreatic tissue at dilution of 1:100
Western Blot Positive WB detected in:HepG2 whole cell lysate,Jurkat whole cell lysate All Lanes: RUNX1T1 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 68,65,69,64,49,45 kDa Observed band size: 68 kDa

RUNX1T1 / ETO Antibody (HRP) LS-C679382
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

RUNX1T1 / ETO Antibody (Biotin) LS-C679379
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

RUNX1T1 / ETO Antibody (FITC) LS-C679383
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00

RUNX1T1 / ETO Antibody (clone OTI1H1) LS-C337730
Mouse Monoclonal [clone OTI1H1] (IgG1) to Human RUNX1T1 / ETO
Human
IF, WB
Unconjugated
100µl 1mg/ml  $345.00
Anti-RUNX1T1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1.
Western blot of extracts (10ug) from 7 different cell lines by using anti-RUNX1T1 monoclonal antibody at 1:200 dilution.

RUNX1T1 / ETO Antibody (clone OTI1H1, Carrier-free) LS-C787137
Mouse Monoclonal [clone OTI1H1] (IgG1) to Human RUNX1T1 / ETO
Human
IF, WB
Unconjugated, Carrier-free
100µg  $395.00
Anti-RUNX1T1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1.
Western blot of extracts (10ug) from 7 different cell lines by using anti-RUNX1T1 monoclonal antibody at 1:200 dilution.

RUNX1T1 / ETO Antibody LS-C753723
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human
ELISA, IHC, WB
Unconjugated
100µg  $355.00

RUNX1T1 / ETO Antibody LS-C406740
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Mouse, Human
ELISA, IHC
Unconjugated
20µl 1.2mg/ml  $205.00; 60µl 1.2mg/ml  $235.00; 120µl 1.2mg/ml  $295.00; 200µl 1.2mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human brain using RUNX1T1 Polyclonal Antibody at dilution of 1:35.

RUNX1T1 / ETO Antibody LS-C412291
Rabbit Polyclonal to Human RUNX1T1 / ETO
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl  $385.00

RUNX1T1 / ETO Antibody LS-C497925
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Mouse, Rat, Human
IHC, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines, using RUNX1T1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

RUNX1T1 / ETO Antibody (N-Terminus) LS-C347705
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human
ChrIP, ELISA
Unconjugated
50µl  $405.00

RUNX1T1 / ETO Antibody (N-Terminus) LS-C146291
Rabbit Polyclonal to Mouse RUNX1T1 / ETO
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
RUNX1T1 antibody Western blot of Mouse Brain lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody (N-Terminus, HRP) LS-C464634
Rabbit Polyclonal to Mouse RUNX1T1 / ETO
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
RUNX1T1 antibody Western blot of Mouse Brain lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody (N-Terminus, Biotin) LS-C464632
Rabbit Polyclonal to Mouse RUNX1T1 / ETO
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
RUNX1T1 antibody Western blot of Mouse Brain lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody (N-Terminus, FITC) LS-C464633
Rabbit Polyclonal to Mouse RUNX1T1 / ETO
Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Gibbon
WB
FITC Conjugated
100µl 1mg/ml  $385.00
RUNX1T1 antibody Western blot of Mouse Brain lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RUNX1T1 / ETO Antibody (N-Terminus) LS-C793472
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human
ELISA
Unconjugated
100µg 0.99708mg/ml  $365.00
ELISA: RUNX1T1 / ETO Antibody
Showing 1-25 of 28 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).