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RTCB / C22orf28

RNA 2',3'-cyclic phosphate and 5'-OH ligase

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.

Gene Name: RNA 2',3'-cyclic phosphate and 5'-OH ligase
Synonyms: RTCB, Ankyrin repeat domain 54, C22orf28, DJ149A16.6, HSPC117, FAAP, RP1-149A16.6
Target Sequences: NM_014306 NP_055121.1 Q9Y3I0

Publications (2)

1
hCLE/RTRAF-HSPC117-DDX1-FAM98B: A New Cap-Binding Complex That Activates mRNA Translation. Pazo A, Pérez-González A, Oliveros JC, Huarte M, Chavez JP, Nieto A. Frontiers in physiology. 2019 February;10:92. [Full Text Article] [PubMed:30833903] [PMC:PMC6388641]
2
hCLE/C14orf166 associates with DDX1-HSPC117-FAM98B in a novel transcription-dependent shuttling RNA-transporting complex. P[Character e9]rez-Gonz[Character e1]lez A, Pazo A, Navajas R, Ciordia S, Rodriguez-Frandsen A, Nieto A. PloS one. 2014 9:e90957. (WB; Human) [Full Text Article] [PubMed:24608264] [PMC:PMC3946611] Related Antibodies: LS-B10474.

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Products
Antibodies (25)
Primary (25)
RTCB / C22orf28 (25)
Human (21)
Mouse (13)
Rat (8)
Bat (8)
Bovine (6)
Chicken (7)
Chimpanzee (6)
Dog (8)
Drosophila (3)
E. coli (4)
Gibbon (6)
Guinea pig (6)
Hamster (1)
Horse (8)
Insect (3)
Monkey (7)
Pig (8)
Rabbit (7)
Xenopus (7)
Zebrafish (3)
IHC (8)
IHC-P (6)
WB (21)
ELISA (10)
IF (2)
IP (2)
Peptide-ELISA (3)
rabbit (22)
goat (3)
Product Group
IHCPlus (2)
IgG (22)
polyclonal pc (25)
Biotin Conjugated (5)
FITC Conjugated (4)
HRP Conjugated (4)
Unconjugated (12)
aa107-156 (4)
aa359-408 (4)
aa125-138 (1)
aa175-225 (1)
aa200-250 (1)
aa201-214 (1)
aa201-215 (1)
aa455-505 (1)
No (24)
Yes (1)
RTCB / C22orf28 Antibody - Human, Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
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RTCB / C22orf28 Goat anti-Human Polyclonal (aa201-214) Antibody
Dog, Pig, Horse, Bat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
RTCB / C22orf28 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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RTCB / C22orf28 Goat anti-Human Polyclonal (aa125-138) Antibody
Xenopus, Chicken, Rabbit, Mouse, Dog, Bat, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
100 µg/$503
Select
RTCB / C22orf28 Rabbit anti-E. coli Polyclonal Antibody
E. coli
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
Select
RTCB / C22orf28 Rabbit anti-E. coli Polyclonal (HRP) Antibody
E. coli
ELISA, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
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RTCB / C22orf28 Rabbit anti-E. coli Polyclonal (Biotin) Antibody
E. coli
ELISA, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
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RTCB / C22orf28 Rabbit anti-E. coli Polyclonal (FITC) Antibody
E. coli
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
RTCB / C22orf28 Antibody - Immunohistochemistry of paraffin-embedded human colon cancer using RTCB Antibody at dilution of 1:100
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RTCB / C22orf28 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$294; 100 µg/$360
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA
FITC Conjugated
50 µg/$294; 100 µg/$360
RTCB / C22orf28 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa107-156) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
RTCB / C22orf28 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa107-156) (HRP) Antibody
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
RTCB / C22orf28 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa107-156) (Biotin) Antibody
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
RTCB / C22orf28 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa107-156) (FITC) Antibody
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IP, WB
Unconjugated
100 µg/$341
RTCB / C22orf28 Antibody - Immunohistochemistry of paraffin-embedded Human lung cancer tissue  using RTCB Polyclonal Antibody at dilution of 1:45(×200)
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RTCB / C22orf28 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
RTCB / C22orf28 Antibody - Detection of human and mouse RTCB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RTCB antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa455-505) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
RTCB / C22orf28 Antibody - C22orf28 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa359-408) Antibody
Human
WB
Unconjugated
100 µl/$424
RTCB / C22orf28 Antibody - C22orf28 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa359-408) (HRP) Antibody
Xenopus, Chicken, Zebrafish, Insect, Drosophila, Bat, Pig, Horse, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Human, Monkey, Gibbon, Chimpanzee
WB
HRP Conjugated
100 µl/$469
RTCB / C22orf28 Antibody - C22orf28 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa359-408) (Biotin) Antibody
Xenopus, Chicken, Zebrafish, Insect, Drosophila, Bat, Pig, Horse, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Human, Monkey, Gibbon, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
RTCB / C22orf28 Antibody - C22orf28 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa359-408) (FITC) Antibody
Xenopus, Chicken, Zebrafish, Insect, Drosophila, Bat, Pig, Horse, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Human, Monkey, Gibbon, Chimpanzee
WB
FITC Conjugated
100 µl/$469
RTCB / C22orf28 Antibody - Western blot analysis of extracts of various cell lines, using RTCB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
RTCB / C22orf28 Antibody - Detection of human and mouse RTCB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RTCB antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa200-250) Antibody
Mouse, Human
WB
Unconjugated
10 µl/$295; 100 µl/$514
RTCB / C22orf28 Antibody - Detection of human and mouse RTCB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RTCB antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Select
RTCB / C22orf28 Rabbit anti-Human Polyclonal (aa175-225) Antibody
Mouse, Human
WB
Unconjugated
10 µl/$295; 100 µl/$514
RTCB / C22orf28 Antibody - RTCB / C22orf28 biotinylated antibody (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Select
RTCB / C22orf28 Goat anti-Human Polyclonal (aa201-215) (Biotin) Antibody
Human
Peptide-ELISA, WB
Biotin Conjugated
100 µg/$503
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).