RPS6KB1 / P70S6K / S6K

ribosomal protein S6 kinase, 70kDa, polypeptide 1

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.

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Proteins (7)

Type

Over-Expression Lysate (2)

Recombinant (5)

Target

RPS6KB1 / P70S6K / S6K (7)

Publications

No (7)

Tag

GST (2)

GST, N-terminus (1)

His-S (1)

Myc-DDK (Flag) (3)

Species

Human (6)

Source

293T Cells (1)

Baculovirus (1)

E. coli (1)

HEK 293 Cells (2)

Wheat Germ Extract (2)

Purification

Purified (2)

Low Endotoxin Level

Low endotoxin level (1)

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Human RPS6KB1 / P70S6K / S6K Protein (Recombinant GST, N-terminus) (aa416-525) - LS-G59980

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human RPS6KB1 / P70S6K / S6K Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G66892

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

59 kDa

Price:

20 µg/$1,107

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Human RPS6KB1 / P70S6K / S6K Protein (Recombinant GST) (Met1-Leu525) - LS-G97837

Source:

Baculovirus

Tag:

GST

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

85.4 kDa

Price:

50 µg/$502

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Human RPS6KB1 / P70S6K / S6K Protein (Recombinant GST) (Full Length) - LS-G30260

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

85.5 kDa

Price:

10 µg/$479; 25 µg/$670

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Human RPS6KB1 / P70S6K / S6K Protein (Recombinant His-S) (aa91-352) - LS-G26159

Source:

E. coli

Tag:

His-S

Molecular Weight:

33.2 kDa

Price:

10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119

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RPS6KB1 / P70S6K / S6K Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G99980

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

59 kDa

Price:

100 µg/$710

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Human RPS6KB1 / P70S6K / S6K Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G78667

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

59 kDa

Price:

20 µg/$215

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