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RNF7

ring finger protein 7

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.

Gene Name: ring finger protein 7
Synonyms: RNF7, CKII beta-binding protein 1, CKBBP1, RING-box protein 2, Rbx2, Regulator of cullins 2, Ring finger protein 7, ROC2, Zinc RING finger protein SAG
Target Sequences: NM_014245 NP_055060.1 Q9UBF6

Publications (3)

1
Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG, Elledge SJ, Conaway RC, Harper JW, Conaway JW. Science (New York, N.Y.). 1999 284:657-61. [PubMed:10213691] Related Antibodies: LS-C19102.
2
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Molecular cell. 1999 3:535-41. [PubMed:10230407] Related Antibodies: LS-C19102.
3
Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Deshaies RJ, Shevchenko A, Deshaies RJ. Genes & development. 1999 13:1614-26. [PubMed:10385629] [PMC:PMC316801] Related Antibodies: LS-C19102.

☰ Filters
Antibodies (10)
Proteins (7)
ELISA Kits (3)
Over-Expression Lysate (2)
Primary (10)
Recombinant (5)
Sandwich (3)
RNF7 (20)
Human (12)
Mouse (5)
Rat (3)
Bat (1)
Bovine (1)
Chicken (1)
Dog (1)
Horse (1)
Monkey (1)
Pig (1)
Xenopus (1)
Zebrafish (1)
IHC (4)
IHC-P (2)
WB (9)
ELISA (6)
IF (3)
IP (1)
Peptide-ELISA (2)
rabbit (9)
goat (1)
Product Group
IHCPlus (1)
IgG (7)
polyclonal pc (10)
96-Well Strip Plate (3)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
Unconjugated (7)
aa102-113 (1)
aa25-57 (1)
aa59-71 (1)
No (19)
Yes (1)
Plasma (3)
Serum (3)
Tissue Homogenates (1)
GST (2)
His (2)
Myc-DDK (Flag) (3)
Human (6)
293T Cells (1)
E. coli (2)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Yeast (1)
Purified (2)
Select
RNF7 Rabbit anti-Human Polyclonal (aa102-113) Antibody
Human
ELISA, IHC, IP, WB
Unconjugated
100 µl/$567
RNF7 Antibody - Anti-RNF7 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Select
RNF7 Goat anti-Human Polyclonal (aa59-71) Antibody
Xenopus, Chicken, Zebrafish, Pig, Horse, Mouse, Dog, Bovine, Bat, Rat, Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
RNF7 Antibody - Immunofluorescence analysis of MCF7 cells.
Select
RNF7 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
RNF7 Antibody - Antibody staining RNF7 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Select
RNF7 Rabbit anti-Human Polyclonal (aa25-57) Antibody
Mouse, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
200 µl/$393
Select
RNF7 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$341
RNF7 Antibody - IHC image of RNF7 Antibody diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
RNF7 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
Select
RNF7 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
RNF7 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
RNF7 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
Select
RNF7 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
RNF7 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.312 - 20 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
RNF7 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Mouse
0.312 - 20 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
RNF7 Protein - Purified recombinant protein RNF7 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
12.5 kDa
20 µg/$1,107
RNF7 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
12.5 kDa
20 µg/$150
RNF7 Protein
Select
Yeast
His
14.5 kD
100 µg/$446; 20 µg/$336; 1 mg/$1,460
Select
E. coli
GST
39.9 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
RNF7 Protein - 12.5% SDS-PAGE of human RNF7 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
38.17 kDa
10 µg/$479; 25 µg/$670
RNF7 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
31.25 - 2000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$579
RNF7 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
12.5 kDa
100 µg/$494
RNF7 Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
Viewing 1-20 of 20 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).