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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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RFX5

regulatory factor X, 5 (influences HLA class II expression)

A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.

Gene Name: regulatory factor X, 5 (influences HLA class II expression)
Synonyms: RFX5, Binding regulatory factor, DNA-binding protein RFX5, Regulatory factor X 5
Target Sequences: NM_000449 NP_000440.1 P48382

Viewing 1-25 of 35 items
RFX5 Antibody - Immunofluorescence analysis of U20S cells.
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
IF, IHC, WB
Unconjugated
50µl   $235.00; 100µl   $295.00; 200µl   $385.00
Select
Rabbit Polyclonal to Human RFX5
Human
IF, IHC, WB
Unconjugated
100µl   $385.00
RFX5 Antibody - Immunohistochemistry of paraffin-embedded human rectal cancer using antibody at 1:100 dilution.
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, IHC, IHC-P
Unconjugated
50µl   $285.00; 100µl   $385.00
Select
Rabbit Polyclonal to Human RFX5
Human
ELISA, IP, WB
Unconjugated
100µg   1mg/ml   $425.00
Select
Rabbit Polyclonal to Human RFX5
Human
ELISA, IP, WB
Unconjugated
100µg   1mg/ml   $425.00
Select
Rabbit Polyclonal to Human RFX5
Human
ELISA, IP, WB
Unconjugated
100µl   90mg/ml   $425.00
RFX5 Antibody - Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.
Select
Rabbit Polyclonal to Human RFX5
Human
ELISA, IP, WB
Unconjugated
100µg   1mg/ml   $425.00
RFX5 Antibody - Detection limit for recombinant GST tagged RFX5 is 0.1 ng/ml as a capture antibody.
Select
Mouse Monoclonal [clone 3B8] (IgG2a,k) to Human RFX5
Human
ELISA, WB
Unconjugated
100µg   $365.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, WB
Biotin Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, WB
PE Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, WB
APC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, WB
AP Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
ELISA, WB
Unconjugated
100µg   $375.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
IP, WB
Unconjugated
100µl   1mg/ml   $395.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
IP, WB
Unconjugated
100µg   $755.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
IP, WB
Unconjugated
100µl   $755.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
IP, WB
Unconjugated
100µg   $755.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
IP, WB
Unconjugated
100µl   $755.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
IP, WB
Unconjugated
100µl   $755.00
Select
Rabbit Polyclonal (IgG) to Human RFX5
Human
IF
Unconjugated
60µl   1mg/ml   $235.00; 120µl   1mg/ml   $295.00; 200µl   1mg/ml   $395.00
RFX5 Antibody - RFX5 antibody (2 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Select
Goat Polyclonal to Human RFX5
Human
Peptide-ELISA, WB
Unconjugated
100µg   0.5mg/ml   $405.00
Select
Rabbit Polyclonal to Human RFX5
Human
ELISA
Unconjugated
100µl   85mg/ml   $425.00

Viewing 1-25 of 35 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).