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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RFX / RFX1

regulatory factor X, 1 (influences HLA class II expression)

RFX / RFX1 is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.

Gene Name: regulatory factor X, 1 (influences HLA class II expression)
Synonyms: RFX1, Enhancer factor C, EFC, Regulatory factor X 1, RFX, Transcription factor RFX1, EF-C
Target Sequences: NM_002918 NP_002909.4 P22670

Filtered by: None
Target
RFX / RFX1 (31)
Reactivity
Human (27)
Mouse (9)
Rat (8)
Bat (4)
Bovine (12)
Chimpanzee (9)
Dog (12)
Gibbon (9)
Guinea pig (8)
Monkey (4)
Pig (12)
Application
IHC (3)
IHC-P (2)
WB (27)
ELISA (9)
ICC (1)
IF (2)
IP (2)
Host
rabbit (31)
Isotype
IgG (26)
Clonality
polyclonal pc (31)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (6)
FITC Conjugated (6)
HRP Conjugated (6)
PE Conjugated (1)
Unconjugated (10)
Epitope
aa896-925 (8)
C-Terminus (4)
aa181-230 (4)
aa287-336 (4)
aa38-87 (4)
aa200-250 (1)
aa929-979 (1)
Publications
No (31)
Showing 1-25 of 31 results

IHC-plus™ RFX / RFX1 Antibody LS-B13083
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
0.05mg 1mg/ml  $425.00
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 ug/mL.

RFX / RFX1 Antibody (aa181-230) LS-C116025
Rabbit Polyclonal to Mouse RFX / RFX1
Mouse, Dog, Guinea pig, Bovine, Rat, Pig
WB
Unconjugated
100µl 0.5mg/ml  $365.00
RFX1 antibody Western blot of Mouse Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa181-230, HRP) LS-C434994
Rabbit Polyclonal to Mouse RFX / RFX1
Mouse, Dog, Guinea pig, Bovine, Rat, Pig
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
RFX1 antibody Western blot of Mouse Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa181-230, Biotin) LS-C434991
Rabbit Polyclonal to Mouse RFX / RFX1
Mouse, Dog, Guinea pig, Bovine, Rat, Pig
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
RFX1 antibody Western blot of Mouse Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa181-230, FITC) LS-C434993
Rabbit Polyclonal to Mouse RFX / RFX1
Mouse, Dog, Guinea pig, Bovine, Rat, Pig
WB
FITC Conjugated
100µl 1mg/ml  $385.00
RFX1 antibody Western blot of Mouse Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa38-87) LS-C80186
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Dog, Pig, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa38-87, Biotin) LS-C436624
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Dog, Pig, Bat, Gibbon, Human, Monkey, Chimpanzee
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody LS-C670671
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image at a dilution of 1:700 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:700 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

RFX / RFX1 Antibody (aa929-979) LS-C289748
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Mouse, Human
IHC, IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00

RFX / RFX1 Antibody (aa896-925) LS-C162557
Rabbit Polyclonal to Human RFX / RFX1
Human
IF, WB
Unconjugated
400µl  $325.00
Fluorescent image of U251 cell stained with RFX1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). RFX1 immunoreactivity is localized to Nucleus significantly.
RFX1 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The RFX1 antibody detected the RFX1 protein (arrow).

RFX / RFX1 Antibody (aa896-925) LS-C324628
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, WB
Unconjugated
200µl  $590.00

RFX / RFX1 Antibody (aa896-925, Biotin) LS-C268113
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

RFX / RFX1 Antibody (aa896-925, FITC) LS-C268114
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, WB
FITC Conjugated
200µl  $740.00

RFX / RFX1 Antibody (aa896-925, APC) LS-C268112
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, WB
APC Conjugated
200µl  $740.00

RFX / RFX1 Antibody (aa896-925, PE) LS-C268116
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, WB
PE Conjugated
200µl  $740.00

RFX / RFX1 Antibody (aa896-925, AP) LS-C268111
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, WB
AP Conjugated
200µl  $740.00

RFX / RFX1 Antibody (aa896-925, Azide-free, HRP) LS-C268115
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

RFX / RFX1 Antibody (C-Terminus) LS-C82041
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Dog, Guinea pig, Bovine, Human
WB
Unconjugated
100µl 0.5mg/ml  $365.00
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (C-Terminus, HRP) LS-C481786
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Dog, Guinea pig, Bovine, Human, Chimpanzee, Gibbon
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (C-Terminus, Biotin) LS-C481784
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Dog, Guinea pig, Bovine, Human, Chimpanzee, Gibbon
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa287-336) LS-C101681
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Mouse, Bovine, Rat, Pig, Human
WB
Unconjugated
100µl 0.5mg/ml  $385.00
RFX1 antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa287-336, HRP) LS-C436628
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Mouse, Bovine, Rat, Pig, Gibbon, Chimpanzee, Human
WB
HRP Conjugated
100µl 0.65mg/ml  $405.00
RFX1 antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa287-336, Biotin) LS-C436630
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Mouse, Bovine, Rat, Pig, Gibbon, Chimpanzee, Human
WB
Biotin Conjugated
100µl 1mg/ml  $405.00
RFX1 antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa287-336, FITC) LS-C436627
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Mouse, Bovine, Rat, Pig, Gibbon, Chimpanzee, Human
WB
FITC Conjugated
100µl 1mg/ml  $405.00
RFX1 antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

RFX / RFX1 Antibody (aa200-250) LS-C289745
Rabbit Polyclonal (IgG) to Human RFX / RFX1
Human
IP
Unconjugated
100µl 1mg/ml  $395.00
Showing 1-25 of 31 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).