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RBP3 / IRBP

retinol binding protein 3, interstitial

Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.

Gene Name: retinol binding protein 3, interstitial
Synonyms: RBP3, D10S66, D10S64, IRBP, RBPI, Retinol-binding protein 3, D10S65
Target Sequences: NM_002900 NP_002891.1 P10745

Publications (1)

1
Effects of Ranibizumab and Aflibercept on Human Müller Cells and Photoreceptors under Stress Conditions. Shen W, Yau B, Lee SR, Zhu L, Yam M, Gillies MC. International journal of molecular sciences. 2017 March;18:pii E533. [Full Text Article] [PubMed:28257068] [PMC:PMC5372549]

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Proteins (13)
Over-Expression Lysate (2)
Recombinant (11)
RBP3 / IRBP (13)
No (13)
6His, N-terminus (2)
GST (1)
GST, N-terminus (1)
His (2)
His, N-Terminal (1)
His-T7 (3)
Myc-DDK (Flag) (3)
Bovine (2)
Human (6)
Mouse (2)
Rat (2)
293T Cells (1)
E. coli (8)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Yeast (1)
Low endotoxin level (2)
RBP3 / IRBP Protein - Recombinant Retinol Binding Protein 3, Interstitial By SDS-PAGE
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E. coli
His, N-Terminal
34.2 kDa
10 µg/$262; 50 µg/$339; 100 µg/$512; 200 µg/$642; 1 mg/$1,623; 500 µg/$1,214; 5 mg/$2,562; 2 mg/$1,796
RBP3 / IRBP Protein - Purified recombinant protein RBP3 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
133.4 kDa
20 µg/$1,107
RBP3 / IRBP Protein - Recombinant Retinol Binding Protein 3, Interstitial By SDS-PAGE
Select
E. coli
His-T7
15.8 kDa
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
RBP3 / IRBP Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
35.2kD
10 µg/$281; 50 µg/$395; 500 µg/$1,585; 1 mg/$2,157
RBP3 / IRBP Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
135.36 kDa
20 µg/$150
RBP3 / IRBP Protein - Recombinant Retinol Binding Protein 3, Interstitial By SDS-PAGE
Select
E. coli
His-T7
26.1 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
RBP3 / IRBP Protein
Select
Yeast
His
34.4 kD
100 µg/$679; 20 µg/$429; 1 mg/$1,973
Select
E. coli
GST
59.8 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
Select
E. coli
6His, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
35.2 kDa
10 µg/$281; 50 µg/$389
RBP3 / IRBP Protein - Recombinant Retinol Binding Protein 3, Interstitial By SDS-PAGE
Select
E. coli
His-T7
23.7 kDa
10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176
RBP3 / IRBP Protein - Recombinant Retinol Binding Protein 3, Interstitial By SDS-PAGE
Select
E. coli
His
34.5 kDa
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
RBP3 / IRBP Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
135.36 kDa
100 µg/$494
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).