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Unfortunately, the antibody (ID:210829) is no longer available.

Below is a list of antibodies to the same protein target. You may also try your search again using the search box at the top of the page. Please contact us if you have any questions.



RB1 / Retinoblastoma / RB

retinoblastoma 1

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Gene Name: retinoblastoma 1
Synonyms: RB1, p105-Rb, Osteosarcoma, PRb, Retinoblastoma, Retinoblastoma 1, OSRC, RB, Pp110
Target Sequences: NM_000321 NP_000312.2 P06400

Publications (2)

1
Clinicopathologic features of CDK6 translocation-associated B-cell lymphoproliferative disorders. Chen D, Law ME, Theis JD, Gamez JD, Caron LB, Vrana JA, Dogan A. The American journal of surgical pathology. 2009 33:720-9. (IHC) [PubMed:19145199] [PMC:PMC2674112]
2
Comparative analysis of Rb1, P16 and ER as diagnostic, prognostic and potential targets for therapeutic agents in ovarian epithelial tumors: an immunohistochemical study of 130 ovarian carcinomas. Ferguson DC, Long DJ, Smith MC, Craig-Owens LD, Means J, Fadare O, Desouki MM. Journal of ovarian research. 2015 8:34. (WB; Human) [Full Text Article] [PubMed:26043844] [PMC:PMC4459058] Related Antibodies: LS-B1495.

Filtered By: None
Target
rb1-retinoblastoma-rb (305)
Reactivity
Human (303)
Mouse (107)
Rat (85)
Bat (9)
Bovine (19)
Chicken (12)
Chimpanzee (9)
Dog (19)
Fish (1)
Gibbon (9)
Guinea pig (8)
Horse (7)
Monkey (18)
Pig (15)
Rabbit (8)
Zebrafish (1)
Application
IHC (207)
IHC-Fr (4)
IHC-P (46)
WB (226)
Flo (38)
DB (34)
ELISA (142)
ICC (9)
IF (43)
IP (58)
Peptide-ELISA (21)
Host
rabbit (251)
mouse (54)
Isotype
IgG (191)
IgG1 (45)
IgG1,k (4)
IgG2a (2)
IgG2a,k (1)
IgG2b,k (2)
Clonality
monoclonal mc (55)
polyclonal pc (250)
Clone
10H9 (1)
11C5 (1)
172C1094 (2)
1B1 (1)
1C3 (1)
1D1 (1)
1E5 (1)
1F8 (4)
1G5 (1)
24A7 (1)
28B5 (1)
2C4 (2)
3C8 (1)
3F11 (1)
3H9 (1)
4G2 (1)
4H6 (1)
51B7 (2)
7E4B8 (1)
7F12 (1)
9E10 (1)
RB1AD56A (2)
SPM353 (1)
XZ133 (2)
Format
AP Conjugated (17)
APC (2)
APC Conjugated (19)
Azide-free (18)
Biotin Conjugated (24)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (24)
HRP Conjugated (23)
PE Conjugated (19)
Ready-to-Use (1)
Unconjugated (175)
Epitope
C-Terminus (33)
pSer780 (20)
pSer807 (16)
pSer811 (14)
pSer608 (13)
pSer612 (11)
pThr821 (11)
pThr826 (11)
pSer249 (10)
pSer788 (10)
aa753-928 (9)
pSer795 (9)
Internal (8)
aa639-778 (8)
pThr252 (7)
aa309-590 (6)
N-Terminus (5)
aa393-572 (5)
aa621-670 (4)
aa703-752 (4)
PSer249/Thr252 (2)
aa190-270 (2)
Cleaved (1)
Gly401 (1)
Pro789 (1)
Ser807 (1)
Thr601 (1)
Thr774 (1)
aa121-140 (1)
aa196-245 (1)
aa211-260 (1)
aa221-270 (1)
aa251-268 (1)
aa280-300 (1)
aa371-390 (1)
aa41-330 (1)
aa511-530 (1)
aa550-600 (1)
aa550-630 (1)
aa552-601 (1)
aa570-650 (1)
aa571-590 (1)
aa581-630 (1)
aa601-650 (1)
aa649-928 (1)
aa720-800 (1)
aa730-810 (1)
aa740-820 (1)
aa750-830 (1)
aa760-840 (1)
aa761-810 (1)
aa771-820 (1)
aa781-830 (1)
aa791-840 (1)
aa858-886 (1)
aa878-928 (1)
aa886-905 (1)
pSer807/811 (1)
pSer821 (1)
pSer826 (1)
Publications
No (304)
Yes (1)
Showing 1-25 out of 305 results
Anti-RB1 / Retinoblastoma / RB Antibody (N-Terminus) IHC-plus™ LS-B1495
Anti-RB1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1495 dilution 1:100.
Anti-RB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1495 dilution 1:100.

Rabbit Polyclonal (IgG) to Human RB1 / Retinoblastoma / RB
Mouse, Human
IF IHC IHC-P WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (aa886-905, clone 3C8) LS-C83414

Mouse Monoclonal [clone 3C8] (IgG2a) to Human RB1 / Retinoblastoma / RB
Human
ELISA IHC IP WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody LS-C497669
Immunohistochemistry of paraffin-embedded mouse spleen tissue.
Immunohistochemistry of paraffin-embedded human mammary cancer tissue.

Rabbit Monoclonal (IgG) to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human
ICC IF IHC WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (C-Terminus) LS-C50693
Colon carcinoma stained with Anti-RB antibody

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Human
IHC
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (C-Terminus) LS-C352774
Immunohistochemical analysis of RB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of RB1 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Mouse, Dog, Bovine, Rat, Pig, Human
ICC IF IHC IHC-P IP WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody LS-C51764

Mouse Monoclonal (IgG1) to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (clone 7E4B8) LS-C41401
IHC of paraffin-embedded human normal esophagus (A) and stomach (B) tissue, showing nucleus localization using Rb mouse monoclonal antibody with DAB staining.

Mouse Monoclonal [clone 7E4B8] (IgG1) to Human RB1 / Retinoblastoma / RB
Human
ELISA IHC
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (phospho-Ser780, clone 2C4) LS-C85365

Mouse Monoclonal [clone 2C4] (IgG1) to Human RB1 / Retinoblastoma / RB
Human
ELISA WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (phospho-Ser780) LS-C96958
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Dot blot of anti-Phospho-Rb-S780 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Human
DB IHC IHC-P
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody LS-C182428

Rabbit Polyclonal (IgG) to Zebrafish RB1 / Retinoblastoma / RB
Fish, Zebrafish
ELISA WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (aa393-572) LS-C26527

Mouse Monoclonal (IgG1)
Chicken, Mouse, Rat, Human
IP WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (aa781-830) IHC-plus™ LS-B7581
Anti-RB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7581 dilution 1:200.
Western blot of extracts from MOLT cells, using Retinoblastoma (Ab-807) Antibody. The lane on the right is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (phospho-Ser780) LS-C352779
Immunohistochemical analysis of RB1 (pS780) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of RB1 (pS780) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Dog, Bovine, Pig, Human
ICC IF IHC IHC-P WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (aa196-245) IHC-plus™ LS-B12499
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of the lysates from HeLa cells using Retinoblastoma antibody.

Rabbit Polyclonal (IgG) to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (C-Terminus) LS-C413081
Immunohistochemical analysis of RB1 (pS807) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of RB1 (pS807) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (C-Terminus) LS-C413192
Immunohistochemical analysis of RB1 staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of RB1 staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Chicken, Mouse, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (C-Terminus) LS-C413080
Immunohistochemical analysis of RB1 (pS795) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of RB1 (pS795) staining in WERI cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (aa552-601) IHC-plus™ LS-B14693
Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of the lysates from COLO205 cells using Retinoblastoma antibody.

Rabbit Polyclonal (IgG) to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (clone 1F8, Azide-free) LS-C87513
Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.

Mouse Monoclonal [clone 1F8] (IgG1) to Human RB1 / Retinoblastoma / RB
Human
Flo IF IHC IHC-P IP WB
Unconjugated, Azide-free
Anti-RB1 / Retinoblastoma / RB Antibody (PSer249/Thr252) LS-C83417

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human
WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (phospho-Thr826) LS-C83423

Rabbit Polyclonal to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human
WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (clone RB1AD56A) LS-C342341
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RB1 mAb.
Western blot analysis of immunized recombinant protein, using anti-RB1 monoclonal antibody.

Mouse Monoclonal [clone RB1AD56A] (IgG1) to Human RB1 / Retinoblastoma / RB
Human
DB ELISA ICC IF WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (phospho-Ser811) LS-C381154

Rabbit Polyclonal (IgG) to Human RB1 / Retinoblastoma / RB
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
Anti-RB1 / Retinoblastoma / RB Antibody (AP) LS-C511067

Mouse Monoclonal (IgG1) to Human RB1 / Retinoblastoma / RB
Human
Flo IF IHC IP WB
AP Conjugated
Anti-RB1 / Retinoblastoma / RB Antibody (APC) LS-C519085

Mouse Monoclonal (IgG1) to Human RB1 / Retinoblastoma / RB
Human
Flo IF IHC IP WB
APC Conjugated

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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