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RAPGEF3 / EPAC

Rap guanine nucleotide exchange factor (GEF) 3

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.

Gene Name: Rap guanine nucleotide exchange factor (GEF) 3
Synonyms: RAPGEF3, Bcm910, CAMP-GEFI, CGEF1, HSU79275, EPAC, EPAC 1, EPAC1
Target Sequences: NM_006105 NP_006096.2 O95398

Publications (1)

1
EPAC1 overexpression is a prognostic marker and its inhibition shows promising therapeutic potential for gastric cancer. Ding-Ping Sun, Chia-Lang Fang, Han-Kun Chen, Kuo-Shan Wen, You-Cheng Hseu, Shih-Ting Hung, Yih-Huei Uen, Kai-Yuan Lin. Oncology reports. 2017 April;37:1953-1960. [Full Text Article] [PubMed:28260059] [PMC:PMC5367365]

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Products
Antibodies (18)
Primary (18)
RAPGEF3 / EPAC (18)
Human (18)
Mouse (16)
Rat (15)
Bovine (4)
Chimpanzee (6)
Dog (4)
Horse (3)
Monkey (6)
Orangutan (3)
Rabbit (6)
IHC (3)
IHC-P (2)
WB (18)
ELISA (4)
ICC (2)
IF (4)
Peptide-ELISA (2)
rabbit (18)
Product Group
IHCPlus (2)
IgG (7)
polyclonal pc (18)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (12)
aa107-156 (4)
aa359-408 (4)
690-770 aa, C-terminal (1)
C-Terminus (1)
aa740-789 (1)
aa799-844 (1)
No (18)
RAPGEF3 / EPAC Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$460
RAPGEF3 / EPAC Antibody - Anti-RAPGEF3 / EPAC antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
RAPGEF3 / EPAC Antibody - RAPGEF3 / EPAC antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa107-156) Antibody
Human
WB
Unconjugated
100 µl/$424
RAPGEF3 / EPAC Antibody - RAPGEF3 / EPAC antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa107-156) (HRP) Antibody
Rabbit, Mouse, Rat, Chimpanzee, Orangutan, Human, Monkey
WB
HRP Conjugated
100 µl/$469
RAPGEF3 / EPAC Antibody - RAPGEF3 / EPAC antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa107-156) (Biotin) Antibody
Rabbit, Mouse, Rat, Chimpanzee, Orangutan, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
RAPGEF3 / EPAC Antibody - RAPGEF3 / EPAC antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa107-156) (FITC) Antibody
Rabbit, Mouse, Rat, Chimpanzee, Orangutan, Human, Monkey
WB
FITC Conjugated
100 µl/$469
RAPGEF3 / EPAC Antibody - Immunofluorescent analysis of EPAC1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Dog, Bovine, Rat, Human
ICC, IF, WB
Unconjugated
100 µl/$299
RAPGEF3 / EPAC Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
RAPGEF3 / EPAC Antibody - Western blot.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa799-844) Antibody
Mouse, Rat, Human
ICC, WB
Unconjugated
50 µl/$321; 100 µl/$403
Select
RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (690-770 aa, C-terminal) Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
Select
RAPGEF3 / EPAC Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
RAPGEF3 / EPAC Antibody - Western blot analysis of extracts from Jurkat and HeLa cells, using RAPGEF3 antibody.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
RAPGEF3 / EPAC Antibody - Western blot analysis of the lysates from HeLa cells using RAPGEF3 antibody.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa740-789) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
RAPGEF3 / EPAC Antibody - RAPGEF3 / EPAC antibody Western blot of HCT15 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa359-408) Antibody
Human
WB
Unconjugated
100 µl/$424
RAPGEF3 / EPAC Antibody - RAPGEF3 / EPAC antibody Western blot of HCT15 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa359-408) (HRP) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Horse, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100 µl/$469
RAPGEF3 / EPAC Antibody - RAPGEF3 / EPAC antibody Western blot of HCT15 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa359-408) (Biotin) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Horse, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
RAPGEF3 / EPAC Antibody - RAPGEF3 / EPAC antibody Western blot of HCT15 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RAPGEF3 / EPAC Rabbit anti-Human Polyclonal (aa359-408) (FITC) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Horse, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100 µl/$469
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).