RAG2 / RAG-2

recombination activating gene 2

Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.

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Proteins (5)

Type

Over-Expression Lysate (2)

Recombinant (3)

Target

RAG2 / RAG-2 (5)

Publications

No (5)

Tag

GST (1)

GST, N-terminus (1)

GST, N-terminus + His, C-terminus (1)

Myc-DDK (Flag) (2)

Species

Human (4)

Source

293T Cells (1)

E. coli (1)

HEK 293 Cells (1)

Wheat Germ Extract (2)

Purification

Purified (1)

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Human RAG2 / RAG-2 Protein (Recombinant GST, N-terminus) (aa428-527) - LS-G59859

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

2 µg/$439

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Human RAG2 / RAG-2 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G79516

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

59.1 kDa

Price:

20 µg/$150

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Human RAG2 / RAG-2 Protein (Recombinant GST) (Full Length) - LS-G30099

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

85.6 kDa

Price:

2 µg/$439

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RAG2 / RAG-2 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G123130

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

59.1 kDa

Price:

100 µg/$494

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Human RAG2 / RAG-2 Protein (Recombinant GST, N-terminus + His, C-terminus) - LS-G145191

Source:

E. coli

Tag:

GST, N-terminus + His, C-terminus

Molecular Weight:

87.1 kDa

Price:

50 µg/$419

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