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PRPF8

pre-mRNA processing factor 8

Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

Gene Name: pre-mRNA processing factor 8
Synonyms: PRPF8, Apoptosis-regulated protein 1, p220, PRP8 homolog, PRP8, PRPC8, RP13, Splicing factor Prp8, Apoptosis-regulated protein 2, HPRP8
Target Sequences: NM_006445 NP_006436.3 Q6P2Q9

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Products
Antibodies (21)
Primary (21)
PRPF8 (21)
Human (21)
Mouse (14)
Rat (8)
Bovine (3)
Chicken (3)
Chimpanzee (3)
Dog (3)
Guinea pig (3)
Horse (3)
Monkey (3)
Pig (3)
Rabbit (3)
Xenopus (3)
Zebrafish (3)
IHC (7)
IHC-P (2)
WB (16)
Flo (3)
DB (2)
ELISA (4)
ICC (3)
IF (2)
IP (2)
Peptide-ELISA (1)
rabbit (18)
mouse (3)
IgG (12)
IgG1 (3)
monoclonal mc (3)
polyclonal pc (18)
2834C1a (2)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (15)
aa360-409 (4)
aa1825-1875 (1)
aa2285-2335 (1)
aa850-900 (1)
No (21)
PRPF8 Antibody - Detection of Human and Mouse PRPF8 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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PRPF8 Antibody (aa1825-1875) LS-C290568
PRPF8 Rabbit anti-Human Polyclonal (aa1825-1875) Antibody
Mouse, Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
PRPF8 Antibody - Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PRPF8 mAb.
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PRPF8 Antibody (clone 2834C1a) LS-C342473
PRPF8 Mouse anti-Human Monoclonal (2834C1a) Antibody
Mouse, Rat, Human
DB, Flo, ICC, IF, WB
Unconjugated
100 µg/$486
PRPF8 Antibody - Immunofluorescent staining using PRPF8 antibody. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-PRPF8 antibody.
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PRPF8 Antibody (clone 2834C1a) LS-C184431
PRPF8 Mouse anti-Human Monoclonal (2834C1a) Antibody
Mouse, Rat, Human
DB, Flo, ICC, WB
Unconjugated
100 µg/$965
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PRPF8 Antibody LS-C75250
PRPF8 Mouse anti-Human Monoclonal Antibody
Mouse, Rat, Human
Flo, ICC, WB
Unconjugated
50 µg/$558
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PRPF8 Antibody LS-C751724
PRPF8 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$331
PRPF8 Antibody - Detection of Human and Mouse PRPF8 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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PRPF8 Antibody (aa2285-2335) LS-C290569
PRPF8 Rabbit anti-Human Polyclonal (aa2285-2335) Antibody
Mouse, Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
PRPF8 Antibody
Select
PRPF8 Antibody LS-C668111
PRPF8 Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P, WB
Unconjugated
50 µl/$286; 100 µl/$321
PRPF8 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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PRPF8 Antibody LS-C802799
PRPF8 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
PRPF8 Antibody - Immunofluorescence staining of Hela cells at a dilution of 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
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PRPF8 Antibody LS-C671461
PRPF8 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF
Unconjugated
50 µg/$294; 100 µg/$360
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PRPF8 Antibody (HRP) LS-C671462
PRPF8 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
PRPF8 Antibody (Biotin) LS-C671467
PRPF8 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
PRPF8 Antibody (FITC) LS-C671466
PRPF8 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
Select
PRPF8 Antibody LS-C769879
PRPF8 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
PRPF8 Antibody - Detection of Human and Mouse PRPF8 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Select
PRPF8 Antibody (aa850-900) LS-C290567
PRPF8 Rabbit anti-Human Polyclonal (aa850-900) Antibody
Mouse, Human
IHC, WB
Unconjugated
10 µl/$295; 100 µl/$514
PRPF8 Antibody - Western blot analysis of 293T cell lysates using PRPF8 Polyclonal Antibody at dilution of 1:450
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PRPF8 Antibody LS-C829997
PRPF8 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
PRPF8 Antibody - Western blot analysis of 293T K562 Raji Hela and NIH/3T3 cell lysates using PRPF8 Polyclonal Antibody at dilution of 1:600
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PRPF8 Antibody LS-C831220
PRPF8 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
PRPF8 Antibody - PRPF8 antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PRPF8 Antibody (aa360-409) LS-C110549
PRPF8 Rabbit anti-Human Polyclonal (aa360-409) Antibody
Human
WB
Unconjugated
100 µl/$424
PRPF8 Antibody - PRPF8 antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PRPF8 Antibody (aa360-409, HRP) LS-C440828
PRPF8 Rabbit anti-Human Polyclonal (aa360-409) (HRP) Antibody
Xenopus, Chicken, Zebrafish, Pig, Horse, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Chimpanzee
WB
HRP Conjugated
100 µl/$469
PRPF8 Antibody - PRPF8 antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PRPF8 Antibody (aa360-409, Biotin) LS-C440237
PRPF8 Rabbit anti-Human Polyclonal (aa360-409) (Biotin) Antibody
Xenopus, Chicken, Zebrafish, Pig, Horse, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
PRPF8 Antibody - PRPF8 antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PRPF8 Antibody (aa360-409, FITC) LS-C440827
PRPF8 Rabbit anti-Human Polyclonal (aa360-409) (FITC) Antibody
Xenopus, Chicken, Zebrafish, Pig, Horse, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey, Chimpanzee
WB
FITC Conjugated
100 µl/$469
Select
PRPF8 Antibody LS-C777866
PRPF8 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC-P
Unconjugated
100 µg/$357
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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