LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

PRKACA + PRKACB

Protein Kinase A, alpha / Protein Kinase A, beta (PRKACA / PRKACB)


PRKACA + PRKACB Target Details

Target Name: Protein Kinase A, alpha / Protein Kinase A, beta (PRKACA / PRKACB)

Filtered By: None
Target
PRKACA + PRKACB (31)
Reactivity
Human (30)
Mouse (28)
Rat (18)
Bovine (9)
Chimpanzee (1)
Dog (2)
Gibbon (1)
Hamster (7)
Monkey (1)
Pig (9)
Sheep (3)
Application
IHC (10)
IHC-P (5)
WB (31)
Flo (1)
DB (1)
ELISA (4)
ICC (1)
IF (6)
IP (1)
Peptide-ELISA (2)
Host
rabbit (30)
mouse (1)
Isotype
IgG (22)
IgG1 (1)
Clonality
monoclonal mc (1)
polyclonal pc (30)
Clone
6D2.1 (1)
Format
AP Conjugated (2)
APC Conjugated (2)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (2)
Unconjugated (19)
Epitope
pThr197 (4)
1-80 aa, N-terminal (1)
Lys17 (1)
N-Terminus (1)
Val191 (1)
aa11 (1)
aa17-66 (1)
aa67-94 (1)
aa8-22 (1)
aa9-40 (1)
pThr198 (1)
Publications
No (31)
Showing 1-25 of 31 results

PRKACA + PRKACB Antibody (aa67-94) LS-C162147
Rabbit Polyclonal to Human PRKACA + PRKACB
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKACA Antibody (N-term K82), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of PRKACA Antibody (N-term K82) in NCI-H460 cell line lysates (35 ug/lane). PRKACA (arrow) was detected using the purified antibody.
Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKACA Antibody (N-term K82), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of PRKACA Antibody (N-term K82) in NCI-H460 cell line lysates (35 ug/lane). PRKACA (arrow) was detected using the purified antibody.

PRKACA + PRKACB Antibody (N-Terminus) LS-C352697
Rabbit Polyclonal to Human PRKACA + PRKACB
Mouse, Dog, Sheep, Bovine, Rat, Pig, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of PKA C alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of PKA C alpha/beta staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of PKA C alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of PKA C alpha/beta staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

PRKACA + PRKACB Antibody (phospho-Thr197) LS-C199444
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of A549 cells, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of A549 cells, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.

PRKACA + PRKACB Antibody (1-80 aa, N-terminal) LS-C385523
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of PKA alpha/beta cat antibody
Western blot of PKA alpha/beta cat antibody
Western blot of PKA alpha/beta cat antibody
Western blot of PKA alpha/beta cat antibody

PRKACA + PRKACB Antibody (Val191) LS-C176451
Rabbit Polyclonal to Human PRKACA + PRKACB
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of PKACa (V191) pAb in paraffin-embedded human colon carcinoma.
Western blot of PKACa (V191) pAb in extracts from mouse brain.
IHC of PKACa (V191) pAb in paraffin-embedded human colon carcinoma.
Western blot of PKACa (V191) pAb in extracts from mouse brain.

PRKACA + PRKACB Antibody LS-C417268
Rabbit Polyclonal to Human PRKACA + PRKACB
Mouse, Rat, Human
IF IHC WB
Unconjugated
100µg 1mg/ml  $305.00

PRKACA + PRKACB Antibody (Lys17) LS-C177118
Rabbit Polyclonal to Human PRKACA + PRKACB
Mouse, Rat, Human
IF IHC WB
Unconjugated
100µl  $345.00
Western blot of PKA antibody in extracts from COLO cells.
Western blot of PKA antibody in extracts from COLO cells.

PRKACA + PRKACB Antibody (aa9-40) LS-C98916
Rabbit Polyclonal to Human PRKACA + PRKACB
Mouse, Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-PKA/C alpha/beta antibody is used in Western blot to detect PKA/C alpha in Jurkat cell lysate (lane 1) and mouse heart tissue lysate (lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-PKA/C alpha/beta antibody is used in Western blot to detect PKA/C alpha in Jurkat cell lysate (lane 1) and mouse heart tissue lysate (lane 2).

PRKACA + PRKACB Antibody (clone 6D2.1) LS-C355223
Mouse Monoclonal [clone 6D2.1] (IgG1) to Human PRKACA + PRKACB
Human, Monkey, Chimpanzee, Gibbon
DB ELISA WB
Unconjugated
100µg  $395.00
Western blot analysis of human protein kinase A catalytic (PKAc) alpha subunit was performed by loading 40 µg of Jlat-full whole cell lysate in reducing sample buffer onto a 4-15% tris-glycine gel. Proteins were transferred to PVDF and then blocked in blocking buffer (TBST+5% non-fat milk) for one hour at room temperature. PKC alpha was detected at approximately 42 kDa using a PKA alpha Monoclonal Antibody (6D2.1) at a dilution of 1:500 overnight at 4°C on a rocking platform, followed by a goat anti-mouse IgG Horseradish Peroxidase-conjugated Antibody at a dilution of 1:2, 500 for one hour at room temperature. Chemiluminescent detection was performed.
DB using PKAc Antibody (6D2.1)
Western blot analysis of human protein kinase A catalytic (PKAc) alpha subunit was performed by loading 40 µg of Jlat-full whole cell lysate in reducing sample buffer onto a 4-15% tris-glycine gel. Proteins were transferred to PVDF and then blocked in blocking buffer (TBST+5% non-fat milk) for one hour at room temperature. PKC alpha was detected at approximately 42 kDa using a PKA alpha Monoclonal Antibody (6D2.1) at a dilution of 1:500 overnight at 4°C on a rocking platform, followed by a goat anti-mouse IgG Horseradish Peroxidase-conjugated Antibody at a dilution of 1:2, 500 for one hour at room temperature. Chemiluminescent detection was performed.
DB using PKAc Antibody (6D2.1)

PRKACA + PRKACB Antibody (phospho-Thr197) LS-C177980
Rabbit Polyclonal to Human PRKACA + PRKACB
Mouse, Rat, Human
IHC WB
Unconjugated
100µl  $345.00
Western blot of p-PKA antibody in extracts from Raw264.7 cells.
Western blot of p-PKA antibody in extracts from Raw264.7 cells.

PRKACA + PRKACB Antibody LS-C408244
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Human
IHC WB
Unconjugated
100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of HeLa cells, using PRKACA antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of HeLa cells, using PRKACA antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

PRKACA + PRKACB Antibody (phospho-Thr197) LS-C75164
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Human
ELISA WB
Unconjugated
10blot  $935.00

PRKACA + PRKACB Antibody LS-C742594
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Rat, Human
ELISA WB
Unconjugated
50µg 1mg/ml  $265.00; 100µg 1mg/ml  $325.00
Western blot analysis of lysates from COLO and Jurkat cells, using KAPC A/B Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO and Jurkat cells, using KAPC A/B Antibody. The lane on the right is blocked with the synthesized peptide.

PRKACA + PRKACB Antibody (aa11) LS-C190239
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Sheep, Bovine, Rat, Pig, Human
IP WB
Unconjugated
100µg 0.5mg/ml  $495.00

PRKACA + PRKACB Antibody (aa17-66) LS-C292113
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Rat, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of the lysates from RAW264.7cells using KAPC A/B antibody.
Western blot analysis of the lysates from RAW264.7cells using KAPC A/B antibody.

PRKACA + PRKACB Antibody (phospho-Thr197) LS-C75165
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Human
WB
Unconjugated
100µl  $665.00

PRKACA + PRKACB Antibody (Biotin) LS-C528937
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Human
WB
Biotin Conjugated
100µl  $860.00

PRKACA + PRKACB Antibody (FITC) LS-C538881
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Human
WB
FITC Conjugated
100µl  $860.00

PRKACA + PRKACB Antibody (APC) LS-C518917
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Human
WB
APC Conjugated
100µl  $860.00

PRKACA + PRKACB Antibody (phospho-Thr198) LS-C411569
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
WB
Unconjugated
50µl  $285.00; 100µl  $375.00; 200µl  $495.00
Western blot analysis of extracts of NIH/3T3 cells.
Western blot analysis of extracts of NIH/3T3 cells.

PRKACA + PRKACB Antibody LS-C416052
Rabbit Polyclonal to Human PRKACA + PRKACB
Mouse, Rat, Human
WB
Unconjugated
100µg 1mg/ml  $305.00
Western blot of Jurkat and K562 cell lysate using KAPC A/B Antibody
Western blot of Jurkat and K562 cell lysate using KAPC A/B Antibody

PRKACA + PRKACB Antibody (aa8-22) LS-C26170
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Dog, Sheep, Bovine, Rat, Hamster, Pig, Human
WB
Unconjugated
200µg  $590.00
Rat heart microsomal preparation probed with 0.5 ug/ml of LS-C26170
Rat heart microsomal preparation probed with 0.5 ug/ml of LS-C26170

PRKACA + PRKACB Antibody (Biotin) LS-C528936
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Bovine, Rat, Hamster, Pig, Human
WB
Biotin Conjugated
100µl  $785.00

PRKACA + PRKACB Antibody (FITC) LS-C538879
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Bovine, Rat, Hamster, Pig, Human
WB
FITC Conjugated
100µl  $785.00

PRKACA + PRKACB Antibody (AP) LS-C510866
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Mouse, Bovine, Rat, Hamster, Pig, Human
WB
AP Conjugated
100µl  $785.00
Showing 1-25 of 31 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy