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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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PRKAA1 / AMPK Alpha 1

protein kinase, AMP-activated, alpha 1 catalytic subunit

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Gene Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
Family/Subfamily: Protein Kinase , AMPK/SNF1
Synonyms: PRKAA1, ACACA kinase, AMPK alpha 1, AMPK alpha-1 chain, Acetyl-CoA carboxylase kinase, AMPKa1, AMPK subunit alpha-1, AMPK, AMPK1, Tau-protein kinase PRKAA1, HMGCR kinase
Target Sequences: NM_006251 NP_006242.5 Q13131

Filtered by: None
Target
PRKAA1 / AMPK Alpha 1 (133)
Reactivity
Human (121)
Mouse (71)
Rat (59)
Bat (8)
Bovine (12)
Chicken (12)
Chimpanzee (9)
Dog (15)
Drosophila (8)
Gibbon (6)
Guinea pig (8)
Hamster (2)
Horse (11)
Monkey (13)
Non-Human Primate (1)
Orangutan (6)
Pig (11)
Primate (1)
Rabbit (14)
Xenopus (10)
Zebrafish (6)
Application
IHC (60)
IHC-Fr (3)
IHC-P (28)
WB (124)
Flo (5)
ELISA (69)
ICC (4)
IF (26)
IP (11)
Peptide-ELISA (2)
Host
rabbit (108)
mouse (25)
Isotype
IgG (73)
IgG1 (3)
IgG1,k (8)
IgG2a (8)
IgG2a,k (2)
Clonality
monoclonal mc (27)
polyclonal pc (106)
Clone
167CT22.1.6 (7)
2B7 (3)
34.2 (1)
4D8 (1)
4D9 (1)
6B6 (1)
84.1 (1)
9G3 (1)
Format
AP Conjugated (6)
APC (1)
APC Conjugated (6)
Azide-free (4)
Biotin Conjugated (12)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (11)
HRP Conjugated (11)
PE Conjugated (7)
Preservative Free (2)
Unconjugated (78)
Epitope
Ser487 (8)
aa198-448 (8)
aa479-510 (8)
N-Terminus (4)
aa110-159 (4)
aa251-300 (4)
aa95-144 (4)
pThr174 (3)
420-500 aa (2)
C-Terminus (2)
aa350-400 (2)
pSer485 (2)
pSer496 (2)
Arg493 (1)
Asn180 (1)
Asp168 (1)
Thr479 (1)
aa376-392 (1)
aa451-500 (1)
pSer485,pSer491 (1)
pSer486 (1)
pSer487 (1)
pThr172 (1)
Publications
No (133)
Showing 1-25 of 133 results

IHC-plus™ PRKAA1 / AMPK Alpha 1 Antibody (aa350-400) LS-B14414
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50µl 1mg/ml  $425.00
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

PRKAA1 / AMPK Alpha 1 Antibody (C-Terminus) LS-C413155
Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Chicken, Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of AMPK alpha 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of AMPK alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

PRKAA1 / AMPK Alpha 1 Antibody (N-Terminus) LS-C126727
Rabbit Monoclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human, Monkey
Flo, IP, WB
Unconjugated
100µl  $530.00

PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7) LS-C169322
Mouse Monoclonal [clone 2B7] (IgG1) to Human PRKAA1 / AMPK Alpha 1
Human
ELISA, Flo, IF, IHC, IHC-P, WB
Unconjugated
100µl  $365.00
IHC of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse monoclonal antibody with DAB staining.
Immunofluorescence of NTERA-2 cells using PRKAA1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

PRKAA1 / AMPK Alpha 1 Antibody (aa251-300) LS-C111536
Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
PRKAA1 / AMPK Alpha 1 antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

PRKAA1 / AMPK Alpha 1 Antibody (phospho-Ser485) LS-C34952
Rabbit Monoclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Hamster, Human, Monkey
WB
Unconjugated
100µl  $575.00

PRKAA1 / AMPK Alpha 1 Antibody (N-Terminus) LS-C122645
Mouse Monoclonal (IgG2a) to Drosophila PRKAA1 / AMPK Alpha 1
Drosophila
ELISA, IF, IHC, IHC-Fr, IP, WB
Unconjugated
100µg  $515.00

PRKAA1 / AMPK Alpha 1 Antibody (Thr479) LS-C175915
Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of AMPK1/AMPK2 (T479) pAb in paraffin-embedded human colon carcinoma tissue.
Western blot of AMPK1/AMPK2 (T479) pAb in extracts from PC12 cells.

PRKAA1 / AMPK Alpha 1 Antibody (phospho-Ser496) LS-C117289
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using AMPK1 (Phospho-Ser485) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with heat shock, using AMPK1 (Phospho-Ser485) Antibody. The lane on the right is blocked with the phospho peptide.

PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7) LS-C359330
Mouse Monoclonal [clone 2B7] (IgG1) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Primate, Human
ELISA, Flo, ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $335.00
Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.
Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7) LS-C758920
Mouse Monoclonal [clone 2B7] (IgG1) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Non-Human Primate, Human
Flo, ICC, IF, IHC, WB
Unconjugated
100µl  $445.00
AMPK alpha-1 Antibody in Immunohistochemistry (IHC)
AMPK alpha-1 Antibody in Immunofluorescence (IF)

PRKAA1 / AMPK Alpha 1 Antibody (aa95-144) LS-C146275
Rabbit Polyclonal to Mouse PRKAA1 / AMPK Alpha 1
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
PRKAA1 / AMPK Alpha 1 antibody Western blot of Mouse Thymus lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PRKAA1 / AMPK Alpha 1 Antibody (phospho-Ser496) LS-C354066
Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Chicken, Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of AMPK alpha 1 (pS496) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of AMPK alpha 1 (pS496) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

PRKAA1 / AMPK Alpha 1 Antibody LS-C500808
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human ovarian cancer using PRKAA1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung tissue using PRKAA1 Antibody at dilution of 1:100

PRKAA1 / AMPK Alpha 1 Antibody (phospho-Ser485,pSer491) LS-C801169
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

PRKAA1 / AMPK Alpha 1 Antibody (phospho-Thr172) LS-C801172
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

PRKAA1 / AMPK Alpha 1 Antibody LS-C659668
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100µg  $405.00

PRKAA1 / AMPK Alpha 1 Antibody LS-C808684
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

PRKAA1 / AMPK Alpha 1 Antibody LS-C808682
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

PRKAA1 / AMPK Alpha 1 Antibody LS-C162140
Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
PRKAA1 Antibody IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of PRKAA1 Antibody in K562, MDA-MB435 cell line lysates (35 ug/lane). PRKAA1 (arrow) was detected using the purified antibody.

IHC-plus™ PRKAA1 / AMPK Alpha 1 Antibody (aa110-159) LS-B10326
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Rabbit, Dog, Human
IHC, IHC-P, WB
Unconjugated
100µl  $485.00
Anti-PRKAA1 / AMPK Alpha 1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PRKAA1 / AMPK Alpha 1 Antibody (420-500 aa) LS-C382033
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of AMPKalpha1 antibody
Western blot of AMPKalpha1 antibody

PRKAA1 / AMPK Alpha 1 Antibody LS-C331372
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry analysis of paraffin-embedded human prostate cancer, using PRKAA1 antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using PRKAA1 antibody at dilution of 1:200 (x400 lens).

IHC-plus™ PRKAA1 / AMPK Alpha 1 Antibody LS-B14126
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
IHC, IHC-P, WB
Unconjugated
40µg 80µl 0.5mg/ml  $425.00
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

PRKAA1 / AMPK Alpha 1 Antibody LS-C746905
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded rat islets of langerhans using PRKAA1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PRKAA1 antibody at dilution of 1:100 (40x lens).
Showing 1-25 of 133 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).