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PPP4R2

protein phosphatase 4, regulatory subunit 2

Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.

Gene Name: protein phosphatase 4, regulatory subunit 2
Synonyms: PPP4R2, PP4R2
Target Sequences: AJ271448 CAB93534.2 Q9NY27

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Products
Antibodies (21)
Primary (21)
PPP4R2 (21)
Human (21)
Mouse (12)
Rat (3)
Bovine (3)
Dog (3)
Horse (3)
Monkey (5)
Orangutan (3)
IHC (3)
WB (21)
ELISA (9)
ICC (1)
IF (2)
IP (1)
Peptide-ELISA (3)
rabbit (21)
IgG (19)
polyclonal pc (21)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (13)
aa228-254 (7)
aa288-337 (4)
140-220 aa, Internal (1)
Internal (1)
aa163-212 (1)
aa171-220 (1)
aa370-C-term (1)
No (21)
PPP4R2 Antibody - Immunofluorescence staining of PPP4R2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PPP4R2 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
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PPP4R2 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, WB
Unconjugated
100 µl/$412
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PPP4R2 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$351
PPP4R2 Antibody - Western blot of PP4R2 antibody
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PPP4R2 Rabbit anti-Human Polyclonal (140-220 aa, Internal) Antibody
Human, Monkey
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
PPP4R2 Antibody - Detection of Human and Mouse PPP4R2 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
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PPP4R2 Rabbit anti-Human Polyclonal (aa370-C-term) Antibody
Mouse, Human
IHC, IP, WB
Unconjugated
2 µg/$295; 100 µl/$514
PPP4R2 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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PPP4R2 Rabbit anti-Human Polyclonal Antibody
Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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PPP4R2 Rabbit anti-Human Polyclonal (aa228-254) Antibody
Mouse, Human
ELISA, WB
Unconjugated
200 µl/$734
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PPP4R2 Rabbit anti-Human Polyclonal (aa228-254) (Biotin) Antibody
Mouse, Human
ELISA, WB
Biotin Conjugated
200 µl/$952
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PPP4R2 Rabbit anti-Human Polyclonal (aa228-254) (FITC) Antibody
Mouse, Human
ELISA, WB
FITC Conjugated
200 µl/$952
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PPP4R2 Rabbit anti-Human Polyclonal (aa228-254) (Azide-free) (HRP) Antibody
Mouse, Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
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PPP4R2 Rabbit anti-Human Polyclonal (aa228-254) (APC) Antibody
Mouse, Human
ELISA, WB
APC Conjugated
200 µl/$952
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PPP4R2 Rabbit anti-Human Polyclonal (aa228-254) (PE) Antibody
Mouse, Human
ELISA, WB
PE Conjugated
200 µl/$952
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PPP4R2 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
100 µg/$357
PPP4R2 Antibody - Western blot analysis of lysates from K562 and COS7 cells, using PPP4R2 Antibody. The lane on the right is blocked with the synthesized peptide.
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PPP4R2 Rabbit anti-Human Polyclonal (aa171-220) Antibody
Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
PPP4R2 Antibody - Western blot analysis of the lysates from COLO205 cells using PPP4R2 antibody.
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PPP4R2 Rabbit anti-Human Polyclonal (aa163-212) Antibody
Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
PPP4R2 Antibody - Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PPP4R2 Rabbit anti-Human Polyclonal (aa288-337) Antibody
Human
WB
Unconjugated
100 µl/$424
PPP4R2 Antibody - Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PPP4R2 Rabbit anti-Human Polyclonal (aa288-337) (HRP) Antibody
Mouse, Dog, Bovine, Rat, Horse, Orangutan, Human, Monkey
WB
HRP Conjugated
100 µl/$469
PPP4R2 Antibody - Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PPP4R2 Rabbit anti-Human Polyclonal (aa288-337) (Biotin) Antibody
Mouse, Dog, Bovine, Rat, Horse, Orangutan, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
PPP4R2 Antibody - Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PPP4R2 Rabbit anti-Human Polyclonal (aa288-337) (FITC) Antibody
Mouse, Dog, Bovine, Rat, Horse, Orangutan, Human, Monkey
WB
FITC Conjugated
100 µl/$469
PPP4R2 Antibody - PPP4R2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The PPP4R2 antibody detected the PPP4R2 protein (arrow).
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PPP4R2 Rabbit anti-Human Polyclonal (aa228-254) Antibody
Mouse, Human
WB
Unconjugated
400 µl/$393
PPP4R2 Antibody - Western blot analysis of PPP4R2 expression in HeLa (A); COS7 (B) whole cell lysates.
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PPP4R2 Rabbit anti-Human Polyclonal (Internal) Antibody
Human, Monkey
WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
PPP4R2 Antibody - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE PPP4R2 antibody diluted at 1:1000
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PPP4R2 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µl/$450
Viewing 1-21 of 21 product results


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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).