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PPP1R8 / Rnase E

protein phosphatase 1, regulatory subunit 8

Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.

Gene Name: protein phosphatase 1, regulatory subunit 8
Synonyms: PPP1R8, ARD-1, ARD1, Activator of RNA decay, NIPP-1, NIPP1, Nuclear subunit of PP-1, PRO2047, RNase E
Target Sequences: NM_002713 NP_002704.1 Q12972

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Products
Proteins (10)
Over-Expression Lysate (4)
Recombinant (6)
PPP1R8 / Rnase E (10)
No (10)
GST (1)
GST, N-terminus (2)
His (1)
Myc-DDK (Flag) (6)
Human (8)
293T Cells (2)
E. coli (2)
HEK 293 Cells (4)
Wheat Germ Extract (2)
Purified (3)
PPP1R8 / Rnase E Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
PPP1R8 / Rnase E Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
GST, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
PPP1R8 / Rnase E Protein - Purified recombinant protein PPP1R8 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
38.3 kDa
20 µg/$1,107
PPP1R8 / Rnase E Protein - Purified recombinant protein PPP1R8 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
22.5 kDa
20 µg/$1,107
PPP1R8 / Rnase E Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
22.5 kDa
20 µg/$150
PPP1R8 / Rnase E Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
38.3 kDa
20 µg/$150
PPP1R8 / Rnase E Protein - 12.5% SDS-PAGE of human PPP1R8 stained with Coomassie Blue
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Wheat Germ Extract
GST
48.73 kDa
2 µg/$439
PPP1R8 / Rnase E Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
22.5 kDa
100 µg/$494
PPP1R8 / Rnase E Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
38.3 kDa
100 µg/$494
PPP1R8 / Rnase E Protein
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E. coli
His
50 µg/$361; 250 µg/$739; 10 µg/$261
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).