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Unfortunately, the antibody (ID:191370) is no longer available.

Below is a list of antibodies to the same protein target. You may also try your search again using the search box at the top of the page. Please contact us if you have any questions.



PPP1R1B / DARPP-32

protein phosphatase 1, regulatory (inhibitor) subunit 1B

PPP1R1B / DARPP-32 is a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: protein phosphatase 1, regulatory (inhibitor) subunit 1B
Family/Subfamily: Protein Phosphatase Regulatory Subunit
Synonyms: PPP1R1B, DARPP-32, DARPP32
Target Sequences: NM_032192 NP_115568.2 Q9UD71

Publications (1)

1
Sex differences in a transgenic rat model of Huntington's disease: decreased 17beta-estradiol levels correlate with reduced numbers of DARPP32+ neurons in males. Bode FJ, Stephan M, Suhling H, Pabst R, Straub RH, Raber KA, Bonin M, Nguyen HP, Riess O, Bauer A, Sjoberg C, Petersn A, von Hrsten S. Human molecular genetics. 2008 17:2595-609. (ICC) [PubMed:18502785]

Filtered By: None
Target
ppp1r1b-darpp-32 (59)
Reactivity
Human (40)
Mouse (47)
Rat (51)
Bovine (6)
Chicken (4)
Dog (5)
Guinea pig (1)
Hamster (1)
Horse (1)
Monkey (3)
Pig (3)
Primate (1)
Rabbit (1)
Sheep (1)
Application
IHC (27)
IHC-Fr (1)
IHC-P (15)
WB (53)
Flo (2)
ELISA (15)
ICC (2)
IF (8)
IP (8)
Host
rabbit (53)
mouse (2)
goat (2)
rat (2)
Isotype
IgG (37)
IgG1 (2)
IgG2a (2)
Clonality
monoclonal mc (5)
polyclonal pc (54)
Clone
141515 (1)
2A4G9 (1)
2G6D9 (1)
Format
AP Conjugated (3)
APC Conjugated (2)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (3)
Unconjugated (42)
Epitope
pThr75 (8)
pThr34 (5)
N-Terminus (4)
aa10-90 (2)
aa51-204 (2)
aa95-204 (2)
Glu160 (1)
Pro69 (1)
aa1-168 (1)
aa1-36 (1)
aa106-139 (1)
aa150-200 (1)
aa18-67 (1)
aa19-50 (1)
aa41-90 (1)
pSer137 (1)
Publications
No (59)
Showing 1-25 out of 59 results
Anti-PPP1R1B / DARPP-32 Antibody IHC-plus™ LS-B14880
Immunohistochemistry of paraffin-embedded rat lung using PPP1R1B antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PPP1R1B antibody at dilution of 1:200 (400x lens).

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa51-204) LS-C36138

Rat Monoclonal (IgG2a) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
ELISA IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody LS-C150127
DARPP32 in Human Brain. DARPP32 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human/Mouse/Rat DARPP32 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4 °C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells.
DARPP32 in Mouse Brain. DARPP32 was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using Goat Anti-Human/Mouse/Rat DARPP32 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4 °C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes.

Goat Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (phospho-Thr34) LS-C25
Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with l-Ptase.

Rabbit Polyclonal to Rat PPP1R1B / DARPP-32
Chicken, Bovine, Mouse, Dog, Rat, Human
IHC WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (phospho-Thr75) LS-C27
Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical

Rabbit Polyclonal to Rat PPP1R1B / DARPP-32
Chicken, Bovine, Mouse, Dog, Rat, Human
IHC WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (Glu160) LS-C36139

Rabbit Monoclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
IF IHC IHC-Fr IHC-P IP WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody LS-C22756

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
ELISA IF WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (phospho-Thr75) LS-C358250
Immunohistochemical analysis of DARPP32 (pT75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of DARPP32 (pT75) expression in MDA-MB-361 (A); SHSY5Y (B) whole cell lysates.

Rabbit Polyclonal to Human PPP1R1B / DARPP-32
Mouse, Rat, Pig, Human
IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa150-200) LS-C203152
Western blot of tissue lysates using 1 ug/ml DARPP-32 Antibody, pAb, Rabbit. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 32 KD. Observed Size: 32 KD.
Flow cytometry of Ramos cells using DARPP-32 Antibody, pAb, Rabbit (shaded histogram). or with an isotype control antibody (open histogram), followed by R-PE conjugated anti-rabbit IgG.

Rabbit Polyclonal to Human PPP1R1B / DARPP-32
Mouse, Rat
Flo WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (N-Terminus) LS-C358835
Immunohistochemical analysis of DARPP32 (pT34) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of DARPP32 (pT34) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human PPP1R1B / DARPP-32
Mouse, Bovine, Pig, Human
ICC IF IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (phospho-Thr34) LS-C416860
Western blot of DARPP-32 phosphorylation expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Rabbit Polyclonal to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
ICC IF IHC WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (phospho-Thr34) LS-C381540
Western blot of Phospho-DARPP-32 (T34) antibody
Western blot of Phospho-DARPP-32 (T34) antibody

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (N-Terminus) LS-C137000

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Human
ELISA IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa18-67) LS-C118090
IHC of paraffin-embedded human brain tissue, using DARPP-32 (Ab-34) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot of extracts from COS7 cells, treated with forskolin 40 nM 30', using DARPP-32 (Ab-34) Antibody. The lane on the right is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
ELISA IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa41-90) LS-C117518
IHC of paraffin-embedded human brain tissue, using DARPP-32 (Ab-75) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, treated with EGF 200 ng/ml 30', using DARPP-32 (Ab-75) Antibody. The lane on the right is treated with the synthesized peptide.

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
ELISA IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa1-36) LS-C408091
DARPP32 was detected in paraffin-embedded sections of mouse pancreas tissues using rabbit anti- DARPP32 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
DARPP32 was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- DARPP32 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

Rabbit Polyclonal to Human PPP1R1B / DARPP-32
Mouse, Dog, Guinea pig, Sheep, Bovine, Rat, Hamster, Human
IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody LS-C490550
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (clone 141515) LS-C150272
Detection of DARPP-32. Mouse brain corpus striatum was dissected, sonicated in 40 volumes of 2x SDS gel sample buffer per weight of tissue, and analyzed by Western Blot. A 15 second exposure to film is shown.

Rat Monoclonal [clone 141515] (IgG2a) to Mouse PPP1R1B / DARPP-32
Mouse
IHC IHC-P WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa95-204, clone 2G6D9) LS-C285885
Western blot using PPP1R1B monoclonal antibody against human PPP1R1B (AA: 95-204) recombinant protein. (Expected MW is 38.3 kDa)
Western blot using PPP1R1B monoclonal antibody against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.

Mouse Monoclonal [clone 2G6D9] (IgG1) to Human PPP1R1B / DARPP-32
Human
ELISA Flo
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (phospho-Thr75) LS-C380678
Western blot of Phospho-DARPP-32 (T75) antibody

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human, Monkey
ELISA IHC WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa10-90) LS-C383076
Western blot of DARPP-32 antibody
Western blot of DARPP-32 antibody

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa10-90) LS-C383078
Western blot of DARPP-32 antibody
Western blot of DARPP-32 antibody

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Mouse, Rat, Human, Monkey
ELISA IHC WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa95-204, clone 2A4G9) LS-C285872
Western blot using PPP1R1B monoclonal antibody against human PPP1R1B (AA: 95-204) recombinant protein. (Expected MW is 38.3 kDa)
Western blot using PPP1R1B monoclonal antibody against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.

Mouse Monoclonal [clone 2A4G9] (IgG1) to Human PPP1R1B / DARPP-32
Human
ELISA WB
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa1-168) LS-C379799
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human epityphlon using antibody at 1:100 dilution.

Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Human
ELISA IHC IHC-P
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (Pro69) LS-C176273
IHC of DARPP-32 (P69) pAb in paraffin-embedded human brain tissue.
Western blot of DARPP-32 (P69) pAb in extracts from 293 cells treated with EGF 200ng/ml 30' or HeLa cells treated with EGF 200ng/ml 5'.

Rabbit Polyclonal to Human PPP1R1B / DARPP-32
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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