Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Reasearch Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order

PPP1R12A / MYPT1

protein phosphatase 1, regulatory subunit 12A

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Gene Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: PPP1R12A, M130, MYPT1, MBS, Myosin binding subunit
Target Sequences: NM_002480 NP_002471.1 O14974

Publications (2)

1
Physiological vs. pharmacological signalling to myosin phosphorylation in airway smooth muscle. Gao N, Tsai MH, Chang AN, He W, Chen CP, Zhu M, Kamm KE, Stull JT. The Journal of physiology. 2017 October;595:6231-6247. [Full Text Article] [PubMed:28749013] [PMC:PMC5621497] Related Antibodies: LS-C117671.
2
Constitutive Phosphorylation of Cardiac Myosin Regulatory Light Chain in vivo. Battiprolu PK, Cowley PM, Chen G, Gerard RD, Pinto JR, Hill JA, Baker AJ, Kamm KE, Stull JT. The Journal of biological chemistry. Mar; [Full Text Article] [PubMed:25733667] Related Antibodies: LS-C117671.

Filtered by: None
Target
PPP1R12A / MYPT1 (66)
Reactivity
Human (66)
Mouse (59)
Rat (43)
Bat (7)
Bovine (3)
Chicken (12)
Chimpanzee (3)
Dog (7)
Gibbon (3)
Guinea pig (6)
Horse (4)
Monkey (6)
Orangutan (3)
Pig (3)
Rabbit (6)
Sheep (2)
Zebrafish (5)
Application
IHC (28)
IHC-P (10)
WB (63)
ELISA (20)
ICC (1)
IF (4)
IP (3)
Peptide-ELISA (5)
Host
rabbit (65)
goat (1)
Isotype
Ig (8)
IgG (42)
Clonality
monoclonal mc (1)
polyclonal pc (65)
Format
AP Conjugated (2)
APC Conjugated (2)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (51)
Epitope
pThr696 (11)
aa845-855, pThr850/853 (6)
aa860-909 (4)
pThr853 (4)
N-Terminus (3)
590-670 aa (2)
630-710 aa (2)
Internal (2)
pSer668 (2)
pThr850 (2)
Arg690 (1)
Arg847 (1)
aa1-17 (1)
aa1-40 (1)
aa400-450 (1)
aa621-670 (1)
aa661-710 (1)
aa800-850 (1)
pSer507 (1)
pThr852 (1)
Publications
No (65)
Yes (1)
Showing 1-25 of 66 results

IHC-plus™ PPP1R12A / MYPT1 Antibody LS-B3836
Goat Polyclonal to Human PPP1R12A / MYPT1
Mouse, Dog, Bovine, Rat, Horse, Bat, Human, Monkey
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PPP1R12A / MYPT1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

PPP1R12A / MYPT1 Antibody (aa800-850) LS-C287730
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Human
IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00

PPP1R12A / MYPT1 Antibody (aa661-710) LS-C117671
Publications (2)
Publications (2)
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYPT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, using MYPT1 Antibody. The lane on the right is blocked with the synthesized peptide.

PPP1R12A / MYPT1 Antibody (N-Terminus) LS-C153530
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µg 100µl 1mg/ml  $315.00

PPP1R12A / MYPT1 Antibody (aa621-670) LS-C117672
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYPT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from NIH/3T3 cells, using MYPT1 Antibody. The lane on the right is blocked with the synthesized peptide.

PPP1R12A / MYPT1 Antibody (phospho-Thr850) LS-C130965
Rabbit Monoclonal (IgG) to Human PPP1R12A / MYPT1
Chicken, Human
WB
Unconjugated
100µl  $620.00

PPP1R12A / MYPT1 Antibody (phospho-Thr696) LS-C199296
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of A549 cells, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is blocked with the phospho peptide.

PPP1R12A / MYPT1 Antibody (phospho-Thr853) LS-C199297
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells, using MYPT1 (Phospho-Thr853) Antibody. The lane on the right is blocked with the phospho peptide.

PPP1R12A / MYPT1 Antibody (phospho-Thr853) LS-C417263
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, WB
Unconjugated
100µg 1mg/ml  $305.00
Western blot of MYPT1 (Phospho-Thr853) expression in NIH/3T3 cell extract

PPP1R12A / MYPT1 Antibody (phospho-Thr850) LS-C91059
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Chicken, Zebrafish, Horse, Rat, Rabbit, Mouse, Dog, Human, Monkey
WB
Unconjugated
200µg  $875.00

PPP1R12A / MYPT1 Antibody LS-C658511
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Human
ICC, IF, IHC, WB
Unconjugated
100µl  $485.00

PPP1R12A / MYPT1 Antibody (aa1-40) LS-C407949
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Dog, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Bat, Human
IHC, IHC-P, WB
Unconjugated
100µg  $355.00
PPP1R12A antibody IHC-paraffin. IHC(P): Human Glioma Tissue.
IHC analysis of PPP1R12A using anti-PPP1R12A antibody. PPP1R12A was detected in immunocytochemical section of U251 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-PPP1R12A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.

PPP1R12A / MYPT1 Antibody (phospho-Thr853) LS-C801515
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunohistochemistry Image

PPP1R12A / MYPT1 Antibody (630-710 aa) LS-C381716
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IF, IHC
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

PPP1R12A / MYPT1 Antibody LS-C801516
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Western blot Image

PPP1R12A / MYPT1 Antibody (590-670 aa) LS-C384731
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

PPP1R12A / MYPT1 Antibody LS-C667027
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Human
ELISA, IHC, WB
Unconjugated
100µg  $335.00

PPP1R12A / MYPT1 Antibody (phospho-Thr696) LS-C800786
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Western blot Image

PPP1R12A / MYPT1 Antibody (590-670 aa) LS-C381250
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Phospho-MYPT1 (T853) antibody
Western blot of Phospho-MYPT1 (T853) antibody

PPP1R12A / MYPT1 Antibody (630-710 aa) LS-C384728
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human, Monkey
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of MYPT1 antibody
Western blot of MYPT1 antibody

PPP1R12A / MYPT1 Antibody LS-C405176
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.5mg/ml  $205.00; 60µl 0.5mg/ml  $235.00; 120µl 0.5mg/ml  $295.00; 200µl 0.5mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human thyroid cancer using PPP1R12A Polyclonal Antibody at dilution of 1:25.
Western blot analysis of NIH/3T3, A172, HeLa and PC3 cell, using PPP1R12A Polyclonal Antibody at dilution of 1:350.

PPP1R12A / MYPT1 Antibody (Arg690) LS-C176432
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of MYPT1 (R690) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of MYPT1 (R690) pAb in extracts from COS7 cells.

PPP1R12A / MYPT1 Antibody (phospho-Thr853) LS-C368470
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Chicken, Zebrafish, Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
30µl 1mg/ml  $215.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $325.00
Immunohistochemical analysis of MYPT1 (pT853) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of MYPT1 (pT853) expression in HEK293T (A); NIH-3T3 (B) whole cell lysates.

PPP1R12A / MYPT1 Antibody (Arg847) LS-C176433
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of MYPT1 (R847) pAb in paraffin-embedded human breast tissue.
Western blot of MYPT1 (R847) pAb in extracts from NIH/3T3 cells.

PPP1R12A / MYPT1 Antibody (Internal) LS-C413123
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Chicken, Zebrafish, Pig, Mouse, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of MYPT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of MYPT1 expression in Jurkat (A); SHSY5Y (B); COLO205 (C) whole cell lysates.
Showing 1-25 of 66 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).