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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPP1R12A / MYPT1

protein phosphatase 1, regulatory subunit 12A

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Gene Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: PPP1R12A, M130, MYPT1, MBS, Myosin binding subunit
Target Sequences: NM_002480 NP_002471.1 O14974

Publications (2)

1
Physiological vs. pharmacological signalling to myosin phosphorylation in airway smooth muscle. Gao N, Tsai MH, Chang AN, He W, Chen CP, Zhu M, Kamm KE, Stull JT. The Journal of physiology. 2017 October;595:6231-6247. [Full Text Article] [PubMed:28749013] [PMC:PMC5621497] Related Antibodies: LS-C117671.
2
Constitutive Phosphorylation of Cardiac Myosin Regulatory Light Chain in vivo. Battiprolu PK, Cowley PM, Chen G, Gerard RD, Pinto JR, Hill JA, Baker AJ, Kamm KE, Stull JT. The Journal of biological chemistry. Mar; [Full Text Article] [PubMed:25733667] Related Antibodies: LS-C117671.


PPP1R12A / MYPT1 Antibody - Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Select
Goat Polyclonal to Human PPP1R12A / MYPT1
Mouse, Dog, Bovine, Rat, Horse, Bat, Human, Monkey
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50µg   0.5mg/ml   $425.00
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Human
IP, WB
Unconjugated
100µl   0.2mg/ml   $405.00
PPP1R12A / MYPT1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYPT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
Select
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µg   $285.00; 100µg   1mg/ml   $355.00
PPP1R12A / MYPT1 Antibody - Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYPT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
Select
Rabbit Monoclonal (IgG) to Human PPP1R12A / MYPT1
Chicken, Human
WB
Unconjugated
100µl   $620.00
PPP1R12A / MYPT1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is blocked with the phospho peptide.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
PPP1R12A / MYPT1 Antibody - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is blocked with the phospho peptide.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
PPP1R12A / MYPT1 Antibody - Western blot of MYPT1 (Phospho-Thr853) expression in NIH/3T3 cell extract
Select
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, WB
Unconjugated
50µg   50µl   1mg/ml   $255.00; 100µg   100µl   1mg/ml   $305.00
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Chicken, Zebrafish, Horse, Rat, Rabbit, Mouse, Dog, Human, Monkey
WB
Unconjugated
200µg   $875.00
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Human
ICC, IF, IHC, WB
Unconjugated
100µl   $485.00
PPP1R12A / MYPT1 Antibody - PPP1R12A antibody IHC-paraffin. IHC(P): Human Glioma Tissue.
Select
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Dog, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Bat, Human
IHC, IHC-P, WB
Unconjugated
100µg   $355.00
PPP1R12A / MYPT1 Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IF, IHC
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
PPP1R12A / MYPT1 Antibody - P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MYPT1 (Phospho-Thr696) antibody.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IF, IHC
Unconjugated
100µg   $305.00
PPP1R12A / MYPT1 Antibody - 1:100 staining mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Human
ELISA, IHC, WB
Unconjugated
100µg   $375.00
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µg   $305.00
PPP1R12A / MYPT1 Antibody - Western blot of MYPT1 antibody
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human, Monkey
ELISA, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
PPP1R12A / MYPT1 Antibody - Western blot of Phospho-MYPT1 (T853) antibody
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
PPP1R12A / MYPT1 Antibody - P-peptide - + Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MYPT1 (Phospho-Thr853) antibody.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µg   $305.00
PPP1R12A / MYPT1 Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using MYPT1 (Ab-853) antibody.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µg   $305.00
PPP1R12A / MYPT1 Antibody - 1:100 staining human thyroid carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human PPP1R12A / MYPT1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
PPP1R12A / MYPT1 Antibody - IHC of MYPT1 (R847) pAb in paraffin-embedded human breast tissue.
Select
Rabbit Polyclonal to Human PPP1R12A / MYPT1
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl   $345.00
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If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).