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PICALM / CALM

phosphatidylinositol binding clathrin assembly protein

PICALM / CALM is a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: phosphatidylinositol binding clathrin assembly protein
Synonyms: PICALM, CALM, LAP, CLTH
Target Sequences: NM_007166 NP_009097.2 Q13492

Publications (1)

1
A single codon insertion in PICALM is associated with development of familial subvalvular aortic stenosis in Newfoundland dogs. Stern JA, White SN, Lehmkuhl LB, Reina-Doreste Y, Ferguson JL, Nascone-Yoder NM, Meurs KM. Human genetics. 2014 133:1139-48. (WB; Human) [Full Text Article] [PubMed:24898977] [PMC:PMC4148152] Related Antibodies: LS-B5065.

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Antibodies (23)
ELISA Kits (4)
Proteins (4)
Custom (3)
Over-Expression Lysate (2)
Primary (23)
Recombinant (2)
Sandwich (1)
PICALM / CALM (31)
Human (24)
Mouse (9)
Rat (13)
Bat (6)
Bovine (6)
Chicken (3)
Chimpanzee (6)
Dog (6)
Gibbon (6)
Guinea pig (6)
Horse (6)
Monkey (6)
Pig (6)
Rabbit (6)
Zebrafish (3)
IHC (4)
IHC-P (3)
WB (19)
ELISA (3)
IF (2)
Peptide-ELISA (3)
rabbit (22)
rabbit-new zealand white (1)
Product Group
IHCPlus (2)
IgG (11)
polyclonal pc (23)
96-Well Strip Plate (4)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
Unconjugated (14)
aa277-326 (4)
aa438-487 (4)
aa385-415 (1)
aa512-543 (1)
aa602-652 (1)
No (30)
Yes (1)
Cell Lysates (1)
Tissue Homogenates (1)
GST (1)
Myc-DDK (Flag) (3)
Human (3)
293T Cells (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (1)
PICALM / CALM Antibody - Anti-PICALM antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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PICALM / CALM Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
PICALM / CALM ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.313 - 20 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Tissue Homogenates
1 Plate/$834
PICALM / CALM Antibody - PICALM antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa438-487) Antibody
Human
WB
Unconjugated
100 µl/$424
PICALM / CALM Antibody - PICALM antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa438-487) (HRP) Antibody
Zebrafish, Rat, Rabbit, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Gibbon, Human, Monkey, Chimpanzee
WB
HRP Conjugated
100 µl/$469
PICALM / CALM Antibody - PICALM antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa438-487) (Biotin) Antibody
Zebrafish, Rat, Rabbit, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Gibbon, Human, Monkey, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
PICALM / CALM Antibody - PICALM antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa438-487) (FITC) Antibody
Zebrafish, Rat, Rabbit, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Gibbon, Human, Monkey, Chimpanzee
WB
FITC Conjugated
100 µl/$469
PICALM / CALM Antibody - Immunohistochemistry image at a dilution of 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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PICALM / CALM Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
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PICALM / CALM Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
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PICALM / CALM Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
Biotin Conjugated
50 µg/$294; 100 µg/$360
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PICALM / CALM Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
PICALM / CALM Antibody - Anti-PICALM / CALM antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
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PICALM / CALM Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
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PICALM / CALM Rabbit-New Zealand White anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
100 µl/$393
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PICALM / CALM Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$357
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PICALM / CALM Rabbit anti-Human Polyclonal Antibody
Rat, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
PICALM / CALM Antibody - Anti-PICALM Antibody (C-Term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa512-543) Antibody
Human
Peptide-ELISA, WB
Unconjugated
200 µl/$393
PICALM / CALM Antibody - All lanes: Anti-PICALM Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysates. Lane 2: A549 whole cell lysates. Lane 3: HeLa whole cell lysates. Lane 4: NIH/3T3 whole cell lysates. Lane 5: rat brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa385-415) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
400 µl/$393
PICALM / CALM Antibody - PICALM antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa277-326) Antibody
Human
WB
Unconjugated
100 µl/$424
PICALM / CALM Antibody - PICALM antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa277-326) (HRP) Antibody
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
HRP Conjugated
100 µl/$469
PICALM / CALM Antibody - PICALM antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa277-326) (Biotin) Antibody
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
PICALM / CALM Antibody - PICALM antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa277-326) (FITC) Antibody
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
FITC Conjugated
100 µl/$469
PICALM / CALM Antibody - Western blot analysis of extracts of various cell lines, using PICALM antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
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PICALM / CALM Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
PICALM / CALM Antibody - Detection of human and mouse PICALM by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PICALM antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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PICALM / CALM Rabbit anti-Human Polyclonal (aa602-652) Antibody
Mouse, Human
WB
Unconjugated
10 µl/$295; 100 µl/$514
PICALM / CALM Antibody - Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE PICALM antibody diluted at 1:500
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PICALM / CALM Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µl/$450
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PICALM / CALM Rabbit anti-Rat Polyclonal Antibody
Rat
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
PICALM / CALM Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
70.6 kDa
100 µg/$494
Viewing 1-25 of 31 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).