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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PIAS1

protein inhibitor of activated STAT, 1

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

Gene Name: protein inhibitor of activated STAT, 1
Synonyms: PIAS1, AR interacting protein, DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, GU/RH-II, Gu-binding protein, GBP, ZMIZ3
Target Sequences: NM_016166 NP_057250.1 O75925

Publications (2)

1
Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response. Conn KL, Wasson P, McFarlane S, Tong L, Brown JR, Grant KG, Domingues P, Boutell C. Journal of virology. 2016 90:4807-26. (ICC, WB; Human) [Full Text Article] [PubMed:26937035] [PMC:PMC4836348]
2
SUMO Ligase Protein Inhibitor of Activated STAT1 (PIAS1) Is a Constituent Promyelocytic Leukemia Nuclear Body Protein That Contributes to the Intrinsic Antiviral Immune Response to Herpes Simplex Virus 1. Brown JR, Conn KL, Wasson P, Charman M, Tong L, Grant K, McFarlane S, Boutell C. Journal of virology. 2016 90:5939-52. (ICC, WB; Human) [Full Text Article] [PubMed:27099310] [PMC:PMC4907222]

Filtered By: None
Target
PIAS1 (47)
Reactivity
Human (47)
Mouse (14)
Rat (7)
Bat (9)
Bovine (8)
Chicken (4)
Chimpanzee (6)
Dog (4)
Gibbon (6)
Guinea pig (4)
Horse (9)
Monkey (9)
Pig (8)
Rabbit (5)
Xenopus (4)
Zebrafish (4)
Application
IHC (35)
IHC-P (8)
WB (46)
Flo (1)
ELISA (19)
ICC (4)
IF (3)
IP (1)
Peptide-ELISA (1)
Host
rabbit (47)
Isotype
IgG (32)
Clonality
polyclonal pc (47)
Format
AP Conjugated (2)
APC (1)
APC Conjugated (3)
Azide-free (2)
Biotin Conjugated (6)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (6)
HRP Conjugated (5)
PE Conjugated (3)
Unconjugated (20)
Epitope
aa409-645 (10)
aa100-130 (8)
aa607-637 (8)
C-Terminus (5)
N-Terminus (5)
10-90 aa, N-terminal (1)
Gly24 (1)
aa10-59 (1)
aa422-651 (1)
aa636-651 (1)
Publications
No (47)
Showing 1-25 of 47 results

PIAS1 Antibody (C-Terminus) IHC-plus™ LS-B4649
Rabbit Polyclonal (IgG) to Human PIAS1
Mouse, Rat, Human
ELISA IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-PIAS1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PIAS1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PIAS1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PIAS1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PIAS1 Antibody (aa636-651) LS-C313346
Rabbit Polyclonal to Human PIAS1
Rabbit, Mouse, Rat, Horse, Bat, Human, Monkey
ICC IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
PIAS1 antibody. ICC: A549 Cell.
PIAS1 antibody. IHC(P): Rat Testis Tissue.
PIAS1 antibody. ICC: A549 Cell.
PIAS1 antibody. IHC(P): Rat Testis Tissue.

PIAS1 Antibody (aa100-130) LS-C328231
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
Unconjugated
200µl 0.25mg/ml  $530.00

PIAS1 Antibody (aa100-130, Biotin) LS-C220358
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

PIAS1 Antibody (aa100-130, FITC) LS-C220359
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
FITC Conjugated
200µl 0.5mg/ml  $680.00

PIAS1 Antibody (aa100-130, PE) LS-C220362
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

PIAS1 Antibody (aa100-130, AP) LS-C220354
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

PIAS1 Antibody (aa100-130, APC) LS-C220356
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

PIAS1 Antibody (aa100-130, Azide-free, HRP) LS-C220360
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

PIAS1 Antibody (N-Terminus) LS-C110359
Rabbit Polyclonal to Human PIAS1
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
50µg  $365.00
PIAS1 antibody Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PIAS1 antibody Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

PIAS1 Antibody LS-C408276
Rabbit Polyclonal (IgG) to Human PIAS1
Mouse, Human
Flo ICC IHC IP WB
Unconjugated
50µl  $315.00; 100µl  $425.00; 200µl  $650.00
Western blot blot of extracts of SW480 cell line, using PIAS1 antibody.
Western blot analysis of extracts of various cell lines, using PIAS1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Western blot blot of extracts of SW480 cell line, using PIAS1 antibody.
Western blot analysis of extracts of various cell lines, using PIAS1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

PIAS1 Antibody LS-C346096
Rabbit Polyclonal (IgG) to Human PIAS1
Mouse, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells.
Immunofluorescence analysis of U2OS cells.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells.
Immunofluorescence analysis of U2OS cells.

PIAS1 Antibody LS-C412511
Rabbit Polyclonal to Human PIAS1
Mouse, Rat, Human
IF IHC WB
Unconjugated
100µl  $385.00

PIAS1 Antibody (aa10-59) LS-C118608
Rabbit Polyclonal (IgG) to Human PIAS1
Mouse, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HUVEC cells, using PIAS1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HUVEC cells, using PIAS1 Antibody. The lane on the right is blocked with the synthesized peptide.

PIAS1 Antibody (aa422-651) LS-C376330
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC IHC-P
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human tonsil using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human tonsil using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.

PIAS1 Antibody (aa100-130) LS-C98792
Rabbit Polyclonal to Human PIAS1
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (RB00668). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (RB00668). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).

PIAS1 Antibody (aa607-637) LS-C328235
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
Unconjugated
200µl 0.25mg/ml  $530.00

PIAS1 Antibody (aa607-637, Biotin) LS-C220352
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

PIAS1 Antibody (aa607-637, APC) LS-C220350
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

PIAS1 Antibody (aa607-637, PE) LS-C220357
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

PIAS1 Antibody (aa607-637, Azide-free, HRP) LS-C220355
Rabbit Polyclonal (IgG) to Human PIAS1
Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

PIAS1 Antibody (10-90 aa, N-terminal) LS-C385486
Rabbit Polyclonal (IgG) to Human PIAS1
Mouse, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of PIAS 1 antibody
Western blot of PIAS 1 antibody

PIAS1 Antibody (N-Terminus) LS-C413098
Rabbit Polyclonal to Human PIAS1
Mouse, Human
IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of PIAS1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of PIAS1 expression in HUVEC (A); Jurkat (B) whole cell lysates.
Immunohistochemical analysis of PIAS1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of PIAS1 expression in HUVEC (A); Jurkat (B) whole cell lysates.

PIAS1 Antibody (aa607-637) LS-C98793
Rabbit Polyclonal to Human PIAS1
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).

PIAS1 Antibody (Gly24) LS-C176341
Rabbit Polyclonal to Human PIAS1
Mouse, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of PIAS1 (G24) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of PIAS1 (G24) pAb in extracts from Jurkat cells.
IHC of PIAS1 (G24) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of PIAS1 (G24) pAb in extracts from Jurkat cells.
Showing 1-25 of 47 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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