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PGK1 / Phosphoglycerate Kinase

phosphoglycerate kinase 1

PGK1/Phosphoglycerate Kinase 1 is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

Gene Name: phosphoglycerate kinase 1
Family/Subfamily: Non-protein Kinase , PGK
Synonyms: PGK1, MIG10, Primer recognition protein 2, PRP 2, PGKA, Phosphoglycerate kinase 1
Target Sequences: NM_000291 NP_000282.1 P00558

Publications (4)

1
Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance. Kilic M, Kasperczyk H, Fulda S, Debatin KM. Oncogene. 2007 26:2027-38. [PubMed:17043658] Related Antibodies: LS-C98055.
2
Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice. Danshina PV, Geyer CB, Dai Q, Goulding EH, Willis WD, Kitto GB, McCarrey JR, Eddy EM, O'Brien DA. Biology of reproduction. 2010 82:136-45. [PubMed:19759366] [PMC:PMC2802118] Related Antibodies: LS-C98055.
3
Mitochondrial impairment in the five-sixth nephrectomy model of chronic renal failure: proteomic approach. Fedorova LV, Tamirisa A, Kennedy DJ, Haller ST, Budnyy G, Shapiro JI, Malhotra D. BMC nephrology. 2013 14:209. (WB; Human) [Full Text Article] [PubMed:24090408] Related Antibodies: LS-B5809.
4
HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Ekman M, Uvelius B, Albinsson S, Sw[Character e4]rd K. Laboratory investigation; a journal of technical methods and pathology. May;94:557-68. [Full Text Article] [PubMed:24589856] Related Antibodies: LS-B5809.
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Antibodies (50)
Primary (50)
PGK1 / Phosphoglycerate Kinase (50)
Human (50)
Mouse (23)
Rat (15)
Bat (5)
Bovine (6)
Chimpanzee (5)
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Gibbon (5)
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Sheep (6)
Zebrafish (1)
IHC (33)
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WB (50)
Flo (8)
ELISA (22)
ICC (6)
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IP (1)
Peptide-ELISA (2)
rabbit (45)
mouse (4)
goat (1)
Product Group
IHCPlus (2)
IgG (30)
IgG1 (1)
IgG1,k (1)
IgG2a,k (2)
monoclonal mc (4)
polyclonal pc (46)
1086CT10.2.1 (1)
2H4 (1)
AT2F4 (1)
APC (1)
APC Conjugated (3)
Biotin Conjugated (5)
Cy3 Conjugated (1)
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HRP Conjugated (6)
PE Conjugated (3)
Unconjugated (25)
aa2-417 (8)
aa117-145 (7)
aa305-334 (7)
aa301-350 (4)
aa102-151 (3)
aa166-180 (1)
aa312-337 (1)
aa321-333 (1)
aa57-308 (1)
No (48)
Yes (2)
PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa102-151) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
PGK1 / Phosphoglycerate Kinase Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa117-145) Antibody
Mouse, Human
Flo, ICC, IF, IHC, IHC-P, WB
Unconjugated
400 µl/$393
PGK1 / Phosphoglycerate Kinase Antibody - Western blot of PGK1/Phosphoglycerate Kinase 1 antibody.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa2-417) Antibody
Pig, Human
ICC, IP, WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
PGK1 / Phosphoglycerate Kinase Antibody - Immunofluorescent analysis of PGK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ICC, IF, WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
PGK1 / Phosphoglycerate Kinase Antibody - PGK1 antibody PGK1(phosphoglycerate kinase 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa301-350) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$397
PGK1 / Phosphoglycerate Kinase Antibody - PGK1 antibody PGK1(phosphoglycerate kinase 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa301-350) (Biotin) Antibody
Rabbit, Dog, Sheep, Bovine, Rat, Bat, Gibbon, Chimpanzee, Orangutan, Human, Monkey
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$442
PGK1 / Phosphoglycerate Kinase Antibody
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PGK1 / Phosphoglycerate Kinase Mouse anti-Human Monoclonal Antibody
Human
ELISA, WB
Unconjugated
100 µl/$445
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa117-145) Antibody
Mouse, Human
ELISA, Flo, IF, IHC, WB
Unconjugated
200 µl/$734
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa117-145) (Biotin) Antibody
Mouse, Human
ELISA, Flo, IF, IHC, WB
Biotin Conjugated
200 µl/$952
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa117-145) (FITC) Antibody
Mouse, Human
Flo, IF, IHC, WB
FITC Conjugated
200 µl/$952
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa117-145) (PE) Antibody
Mouse, Human
ELISA, Flo, ICC, IF, IHC, WB
PE Conjugated
200 µl/$952
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa117-145) (APC) Antibody
Mouse, Human
ELISA, Flo, ICC, IF, IHC, WB
APC Conjugated
200 µl/$952
PGK1 / Phosphoglycerate Kinase Antibody - PGK1 Antibody staining PGK1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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PGK1 / Phosphoglycerate Kinase Mouse anti-Human Monoclonal (1086CT10.2.1) Antibody
Mouse, Human
ELISA, Flo, IHC-P, WB
Unconjugated
200 µl/$393
PGK1 / Phosphoglycerate Kinase Antibody - ICC/IF analysis of PGK1 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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PGK1 / Phosphoglycerate Kinase Mouse anti-Human Monoclonal (AT2F4) Antibody
Human
ELISA, Flo, ICC, WB
Unconjugated
50 µl/$303; 100 µl/$365
PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1/Phosphoglycerate Kinase 1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1/Phosphoglycerate Kinase 1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (HRP) Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1/Phosphoglycerate Kinase 1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (Biotin) Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1/Phosphoglycerate Kinase 1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (FITC) Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded Human cervical cancer using PGK1 Polyclonal Antibody at dilution of 1:50.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded Human liver cancer using PGK1 Polyclonal Antibody at dilution of 1:40.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
PGK1 / Phosphoglycerate Kinase Antibody - Immunofluorescence analysis of HeLa cells.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded rat intestine using PGK1 Antibody at dilution of 1:100 (40x lens).
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa305-334) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$341
PGK1 / Phosphoglycerate Kinase Antibody - PGK1/Phosphoglycerate Kinase 1 antibody. IHC(P): Human Lung Cancer Tissue.
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PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal (aa166-180) Antibody
Zebrafish, Pig, Horse, Rat, Mouse, Guinea pig, Sheep, Bovine, Human, Monkey
IHC, WB
Unconjugated
10 µg/$318; 100 µg/$470
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).