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PFN1 / Profilin 1

profilin 1

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

Gene Name: profilin 1
Synonyms: PFN1, ALS18, Profilin-1, Profilin 1, Profilin I
Target Sequences: NM_005022 NP_005013.1 P07737

Publications (7)

1
Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro. Salazar R, Bell SE, Davis GE. Experimental cell research. 1999 249:22-32. [PubMed:10328950]
2
Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice. Hauser W, Knobeloch KP, Eigenthaler M, Gambaryan S, Krenn V, Geiger J, Glazova M, Rohde E, Horak I, Walter U, Zimmer M. Proceedings of the National Academy of Sciences of the United States of America. 1999 96:8120-5. [PubMed:10393958] [PMC:PMC22198]
3
A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems. Giesemann T, Rathke-Hartlieb S, Rothkegel M, Bartsch JW, Buchmeier S, Jockusch BM, Jockusch H. The Journal of biological chemistry. 1999 274:37908-14. [PubMed:10608857]
4
Endothelin-1 mobilizes profilin-1-bound PIP2 in cardiac muscle. Evans NJ, Walker JW. Experimental biology and medicine (Maywood, N.J.). 2006 231:882-7. [PubMed:16741017]
5
The human submandibular gland: immunohistochemical analysis of SNAREs and cytoskeletal proteins. Stoeckelhuber M, Scherer EQ, Janssen KP, Slotta-Huspenina J, Loeffelbein DJ, Rohleder NH, Nieberler M, Hasler R, Kesting MR. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 2012 60:110-20. [PubMed:22131313] [PMC:PMC3351120]
6
Cerebral adrenoleukodystrophy is associated with loss of tolerance to profilin. Paul J Orchard, David R Nascene, Ashish Gupta, Mandy E Taisto, LeeAnn Higgins, Todd W Markowski, Troy C Lund. European journal of immunology. 2019 June;49:947-953. [Full Text Article] [PubMed:30829395]
7
Actin-binding protein profilin1 promotes aggressiveness of clear-cell renal cell carcinoma cells. Abigail Allen, David Gau, Paul Francoeur, Jordan Sturm, Yue Wang, Ryan Martin, Jodi Maranchie, Anette Duensing, Adam Kaczorowski, Stefan Duensing, Lily Wu, Michael T Lotze, David Koes, Walter J Storkus, Partha Roy. The Journal of biological chemistry. 2020 November;295:15636-15649. [Full Text Article] [PubMed:32883810]
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Proteins (9)
Over-Expression Lysate (2)
Recombinant (7)
PFN1 / Profilin 1 (9)
No (9)
GST (2)
His (1)
His-GST (1)
Myc-DDK (Flag) (3)
Human (6)
Mouse (2)
293T Cells (1)
E. coli (4)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (2)
Low endotoxin level (2)
PFN1 / Profilin 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
14.9 kDa
100 µg/$494
PFN1 / Profilin 1 Protein - 12.5% SDS-PAGE of human PFN1 stained with Coomassie Blue
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Wheat Germ Extract
GST
41.14 kDa
10 µg/$479; 25 µg/$670
PFN1 / Profilin 1 Protein - 12.5% SDS-PAGE of human PFN1 stained with Coomassie Blue
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Wheat Germ Extract
GST
41.14 kDa
10 µg/$479; 25 µg/$670
PFN1 / Profilin 1 Protein - Purified recombinant protein PFN1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
14.9 kDa
20 µg/$1,107
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293T Cells
Myc-DDK (Flag)
14.9 kDa
20 µg/$150
PFN1 / Profilin 1 Protein
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E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$317; 500 µg/$526; 20 µg/$245
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E. coli
None
Less than 0.01 EU/µg protein (determined by LAL method).
~20kDa (SDS-PAGE)
10 µg/$358
PFN1 / Profilin 1 Protein - Recombinant Profilin 1 By SDS-PAGE
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E. coli
His
16.4 kDa
10 µg/$267; 50 µg/$352; 100 µg/$562; 200 µg/$702; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900
PFN1 / Profilin 1 Protein - Recombinant Profilin 1 By SDS-PAGE
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E. coli
His-GST
41.9 kDa
10 µg/$282; 50 µg/$450; 100 µg/$717; 200 µg/$897; 1 mg/$2,010; 500 µg/$1,547; 5 mg/$3,137; 2 mg/$2,198
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).